Insights into Chemical Interactions and Related Toxicities of Deep Eutectic Solvents with Mammalian Cells Observed Using Synchrotron Macro–ATR–FTIR Microspectroscopy

Bryant, SJ; Shaw, ZL; Huang, LZY; Elbourne, A; Abraham, AN; Vongsvivut, J; Holt, SA; Greaves, TL; Bryant, G

Insights into Chemical Interactions and Related Toxicities of Deep Eutectic Solvents with Mammalian Cells Observed Using Synchrotron Macro–ATR–FTIR Microspectroscopy

Bryant, SJ Shaw, ZL Huang, LZY Elbourne, A Abraham, AN Vongsvivut, J Holt, SA Greaves, TL Bryant, G

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Publisher:: MDPI
Publication Type:: Journal Article
Citation:: Biophysica, 2023, 3, (2), pp. 318-334
Issue Date:: 2023-06-01

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Field	Value	Language
dc.contributor.author	Bryant, SJ
dc.contributor.author	Shaw, ZL
dc.contributor.author	Huang, LZY
dc.contributor.author	Elbourne, A
dc.contributor.author	Abraham, AN
dc.contributor.author	Vongsvivut, J
dc.contributor.author	Holt, SA
dc.contributor.author	Greaves, TL
dc.contributor.author	Bryant, G
dc.date.accessioned	2024-03-13T04:16:34Z
dc.date.available	2024-03-13T04:16:34Z
dc.date.issued	2023-06-01
dc.identifier.citation	Biophysica, 2023, 3, (2), pp. 318-334
dc.identifier.issn	2673-4125
dc.identifier.issn	2673-4125
dc.identifier.uri	http://hdl.handle.net/10453/176631
dc.description.abstract	Deep eutectic solvents (DESs) and ionic liquids (ILs) are highly tailorable solvents that have shown a lot of promise for a variety of applications including cryopreservation, drug delivery, and protein stabilisation. However, to date, there is very limited information on the detailed interactions of these solvents with mammalian cells. In this work, we studied six DESs and one IL that show promise as cryoprotective agents, applying synchrotron macro–ATR–FTIR to examine their effects on key biochemical components of HaCat mammalian cells. These data were paired with resazurin metabolic assays and neutron reflectivity experiments to correlate cellular interactions with cellular toxicity. Stark differences were observed even between solvents that shared similar components. In particular, it was found that solvents that are effective cryoprotective agents consistently showed interactions with cellular membranes, while high toxicity correlated with strong interactions of the DES/IL with nucleic acids and proteins. This work sheds new light on the interactions between novel solvents and cells that may underpin future biomedical applications.
dc.language	en
dc.publisher	MDPI
dc.relation	http://purl.org/au-research/grants/arc/CE140100003
dc.relation.ispartof	Biophysica
dc.relation.isbasedon	10.3390/biophysica3020021
dc.rights	info:eu-repo/semantics/openAccess
dc.title	Insights into Chemical Interactions and Related Toxicities of Deep Eutectic Solvents with Mammalian Cells Observed Using Synchrotron Macro–ATR–FTIR Microspectroscopy
dc.type	Journal Article
utslib.citation.volume	3
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Science
pubs.organisational-group	University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access	*
dc.date.updated	2024-03-13T04:16:31Z
pubs.issue	2
pubs.publication-status	Published
pubs.volume	3
utslib.citation.issue	2

Abstract:

Deep eutectic solvents (DESs) and ionic liquids (ILs) are highly tailorable solvents that have shown a lot of promise for a variety of applications including cryopreservation, drug delivery, and protein stabilisation. However, to date, there is very limited information on the detailed interactions of these solvents with mammalian cells. In this work, we studied six DESs and one IL that show promise as cryoprotective agents, applying synchrotron macro–ATR–FTIR to examine their effects on key biochemical components of HaCat mammalian cells. These data were paired with resazurin metabolic assays and neutron reflectivity experiments to correlate cellular interactions with cellular toxicity. Stark differences were observed even between solvents that shared similar components. In particular, it was found that solvents that are effective cryoprotective agents consistently showed interactions with cellular membranes, while high toxicity correlated with strong interactions of the DES/IL with nucleic acids and proteins. This work sheds new light on the interactions between novel solvents and cells that may underpin future biomedical applications.

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http://hdl.handle.net/10453/176631