Investigation of the crystallinity change after the addition of magnesium hydroxides into the calcium phosphate during mechanochemical synthesis: an FTIR spectroscopy, XRD analysis, chemometrics, and cell culture

Otsuka, Y; Sasaki, D; Kusamori, K; Nishikawa, M; Ben-Nissan, B

Investigation of the crystallinity change after the addition of magnesium hydroxides into the calcium phosphate during mechanochemical synthesis: an FTIR spectroscopy, XRD analysis, chemometrics, and cell culture

Otsuka, Y Sasaki, D Kusamori, K Nishikawa, M Ben-Nissan, B

Permalink

Publisher:: Springer Nature
Publication Type:: Journal Article
Citation:: Journal of the Australian Ceramic Society, 2023, 59, (5), pp. 1373-1380
Issue Date:: 2023-12-01

Closed Access

	Filename	Description	Size
	s41779-023-00919-2.pdf	Published version	901.76 kB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Otsuka, Y
dc.contributor.author	Sasaki, D
dc.contributor.author	Kusamori, K
dc.contributor.author	Nishikawa, M
dc.contributor.author	Ben-Nissan, B
dc.date.accessioned	2024-03-13T04:23:07Z
dc.date.available	2024-03-13T04:23:07Z
dc.date.issued	2023-12-01
dc.identifier.citation	Journal of the Australian Ceramic Society, 2023, 59, (5), pp. 1373-1380
dc.identifier.issn	2510-1560
dc.identifier.issn	2510-1579
dc.identifier.uri	http://hdl.handle.net/10453/176633
dc.description.abstract	The aim of this study is to investigate the effect of the addition of Mg ion on calcium phosphate during mechanochemical synthesis. Ca(OH)2, dicalcium phosphate dihydrate (DCPD), and Mg(OH)2 were mechanochemically synthesized using ball milling with pure water. The prepared samples were evaluated by powder X-ray diffraction analysis (XRD), FT-infrared (IR) spectral analysis, and a TNF alpha test with murine macrophage-like cell line RAW264.7 cells for assessing the immune activation. The cell culture studies indicated that CaMg apatite can be used as bioceramic. Mg ion-doped Ca apatite, which also contains carbonated ions in the crystal, was identified based on the XRD patterns and FTIR spectra. The unique diffraction peaks of apatite at 211, 112, 300, and 202 decreased in the sample containing Mg(OH)2, suggesting a decrease in the rate of crystallization. In addition, the phosphate infrared bands of the samples were different from those of the carbonated apatite. To investigate the details of the effects of Mg ion, multiple spectra combining XRD patterns and FTIR spectra were prepared. The multiple spectra datasets were used for the estimation by multivariate curve resolution-alternating least squares (MCR-ALS). The effects of Mg ion on apatite as calcium phosphate were investigated by multiple spectra multivariate analysis. The analysis postulated that Mg ion decreased the apatite crystallinities possibly by adsorption at the growth sides and that these added ions strongly inhibited the formation of the thermodynamically most stable hydroxyapatite.
dc.language	en
dc.publisher	Springer Nature
dc.relation.ispartof	Journal of the Australian Ceramic Society
dc.relation.isbasedon	10.1007/s41779-023-00919-2
dc.rights	info:eu-repo/semantics/closedAccess
dc.title	Investigation of the crystallinity change after the addition of magnesium hydroxides into the calcium phosphate during mechanochemical synthesis: an FTIR spectroscopy, XRD analysis, chemometrics, and cell culture
dc.type	Journal Article
utslib.citation.volume	59
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Science
pubs.organisational-group	University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access	*
dc.date.updated	2024-03-13T04:23:07Z
pubs.issue	5
pubs.publication-status	Published
pubs.volume	59
utslib.citation.issue	5

Abstract:

The aim of this study is to investigate the effect of the addition of Mg ion on calcium phosphate during mechanochemical synthesis. Ca(OH)2, dicalcium phosphate dihydrate (DCPD), and Mg(OH)2 were mechanochemically synthesized using ball milling with pure water. The prepared samples were evaluated by powder X-ray diffraction analysis (XRD), FT-infrared (IR) spectral analysis, and a TNF alpha test with murine macrophage-like cell line RAW264.7 cells for assessing the immune activation. The cell culture studies indicated that CaMg apatite can be used as bioceramic. Mg ion-doped Ca apatite, which also contains carbonated ions in the crystal, was identified based on the XRD patterns and FTIR spectra. The unique diffraction peaks of apatite at 211, 112, 300, and 202 decreased in the sample containing Mg(OH)2, suggesting a decrease in the rate of crystallization. In addition, the phosphate infrared bands of the samples were different from those of the carbonated apatite. To investigate the details of the effects of Mg ion, multiple spectra combining XRD patterns and FTIR spectra were prepared. The multiple spectra datasets were used for the estimation by multivariate curve resolution-alternating least squares (MCR-ALS). The effects of Mg ion on apatite as calcium phosphate were investigated by multiple spectra multivariate analysis. The analysis postulated that Mg ion decreased the apatite crystallinities possibly by adsorption at the growth sides and that these added ions strongly inhibited the formation of the thermodynamically most stable hydroxyapatite.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/176633