Investigation on repairing diabetic foot ulcer based on 3D bio-printing Gel/dECM/Qcs composite scaffolds.
- Publisher:
- Elsevier
- Publication Type:
- Journal Article
- Citation:
- Tissue Cell, 2023, 85, pp. 102213
- Issue Date:
- 2023-12
In Progress
Filename | Description | Size | |||
---|---|---|---|---|---|
1-s2.0-S004081662300201X-main.pdf | Published version | 9.84 MB | Adobe PDF |
Copyright Clearance Process
- Recently Added
- In Progress
- Open Access
This item is being processed and is not currently available.
Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | Zhong, Y | |
dc.contributor.author | Ma, H | |
dc.contributor.author | Lu, Y | |
dc.contributor.author | Cao, L | |
dc.contributor.author | Cheng, YY | |
dc.contributor.author | Tang, X | |
dc.contributor.author | Sun, H | |
dc.contributor.author | Song, K | |
dc.date.accessioned | 2024-05-10T19:22:08Z | |
dc.date.available | 2023-08-30 | |
dc.date.available | 2024-05-10T19:22:08Z | |
dc.date.issued | 2023-12 | |
dc.identifier.citation | Tissue Cell, 2023, 85, pp. 102213 | |
dc.identifier.issn | 0040-8166 | |
dc.identifier.issn | 1532-3072 | |
dc.identifier.uri | http://hdl.handle.net/10453/178829 | |
dc.description.abstract | Diabetic foot ulcers are one of the most serious of the numerous complications of diabetes mellitus, causing great physical trauma and financial stress to patients, and accelerating wound healing in diabetic patients remains one of the major clinical challenges. Exosomes from adipose-derived stem cells can directly and indirectly promote wound healing. However, due to the low retention rate of exosomes in the wound, exosome treatment is difficult to achieve the expected effect. Therefore, it is of great significance to synthesize a composite scaffold that can stably load exosomes and has antibacterial properties. In this study, fresh pig skin was decellularized to obtain decellularized matrix (dECM). Secondly, quaternized chitosan (Qcs) was modified with quaternary ammonium salt to make it soluble in water after quaternization. Finally, Gel-dECM-Qcs (GDQ) bioink was prepared by adding acellular matrix and quaternized chitosan with temperature sensitive gelatin (Gel) as carrier. Tissue engineered composite scaffolds were then prepared by extrusion 3D printing technology. Subsequently, the physicochemical properties, biocompatibility and antimicrobial capacity of the composite scaffolds were determined, and the data showed that the composite scaffolds had good mechanical properties, biocompatibility and antimicrobial capacity, and the maximum stress of the composite scaffolds was 1.16 ± 0.05 MPa, the composite scaffolds were able to proliferate and adhered to the L929 cells, and the kill rates of composite scaffolds against E. coli and S. aureus after incubation for 24 h were 93.24 ± 1.22 % and 97.34 ± 0.23 %, respectively. Overall, the GDQ composite scaffolds have good mechanical properties adapted to skin bending, its good biocompatibility can promote the growth and migration of fibroblasts, reshape injured tissues, accelerate the wound healing, and excellent antimicrobial ability can inhibit the growth of E. coli and S. aureus, reducing the impact of bacterial infections on wounds. Moreover, the composite scaffolds have the potential to be used as exosom-loaded hydrogel dressings, which provides a basis for the subsequent research on the repair of diabetic foot ulcers. | |
dc.format | Print-Electronic | |
dc.language | eng | |
dc.publisher | Elsevier | |
dc.relation.ispartof | Tissue Cell | |
dc.relation.isbasedon | 10.1016/j.tice.2023.102213 | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | 0601 Biochemistry and Cell Biology, 1116 Medical Physiology | |
dc.subject.classification | Biochemistry & Molecular Biology | |
dc.subject.classification | 3101 Biochemistry and cell biology | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Swine | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Chitosan | |
dc.subject.mesh | Gelatin | |
dc.subject.mesh | Diabetic Foot | |
dc.subject.mesh | Escherichia coli | |
dc.subject.mesh | Staphylococcus aureus | |
dc.subject.mesh | Tissue Scaffolds | |
dc.subject.mesh | Anti-Infective Agents | |
dc.subject.mesh | Printing, Three-Dimensional | |
dc.subject.mesh | Diabetes Mellitus | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Swine | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Escherichia coli | |
dc.subject.mesh | Staphylococcus aureus | |
dc.subject.mesh | Diabetic Foot | |
dc.subject.mesh | Diabetes Mellitus | |
dc.subject.mesh | Chitosan | |
dc.subject.mesh | Gelatin | |
dc.subject.mesh | Anti-Infective Agents | |
dc.subject.mesh | Tissue Scaffolds | |
dc.subject.mesh | Printing, Three-Dimensional | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Swine | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Chitosan | |
dc.subject.mesh | Gelatin | |
dc.subject.mesh | Diabetic Foot | |
dc.subject.mesh | Escherichia coli | |
dc.subject.mesh | Staphylococcus aureus | |
dc.subject.mesh | Tissue Scaffolds | |
dc.subject.mesh | Anti-Infective Agents | |
dc.subject.mesh | Printing, Three-Dimensional | |
dc.subject.mesh | Diabetes Mellitus | |
dc.title | Investigation on repairing diabetic foot ulcer based on 3D bio-printing Gel/dECM/Qcs composite scaffolds. | |
dc.type | Journal Article | |
utslib.citation.volume | 85 | |
utslib.location.activity | Scotland | |
utslib.for | 0601 Biochemistry and Cell Biology | |
utslib.for | 1116 Medical Physiology | |
pubs.organisational-group | University of Technology Sydney | |
pubs.organisational-group | University of Technology Sydney/Faculty of Science | |
pubs.organisational-group | University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences | |
pubs.organisational-group | University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices | |
utslib.copyright.status | in_progress | * |
dc.date.updated | 2024-05-10T19:22:02Z | |
pubs.publication-status | Published | |
pubs.volume | 85 |
Abstract:
Diabetic foot ulcers are one of the most serious of the numerous complications of diabetes mellitus, causing great physical trauma and financial stress to patients, and accelerating wound healing in diabetic patients remains one of the major clinical challenges. Exosomes from adipose-derived stem cells can directly and indirectly promote wound healing. However, due to the low retention rate of exosomes in the wound, exosome treatment is difficult to achieve the expected effect. Therefore, it is of great significance to synthesize a composite scaffold that can stably load exosomes and has antibacterial properties. In this study, fresh pig skin was decellularized to obtain decellularized matrix (dECM). Secondly, quaternized chitosan (Qcs) was modified with quaternary ammonium salt to make it soluble in water after quaternization. Finally, Gel-dECM-Qcs (GDQ) bioink was prepared by adding acellular matrix and quaternized chitosan with temperature sensitive gelatin (Gel) as carrier. Tissue engineered composite scaffolds were then prepared by extrusion 3D printing technology. Subsequently, the physicochemical properties, biocompatibility and antimicrobial capacity of the composite scaffolds were determined, and the data showed that the composite scaffolds had good mechanical properties, biocompatibility and antimicrobial capacity, and the maximum stress of the composite scaffolds was 1.16 ± 0.05 MPa, the composite scaffolds were able to proliferate and adhered to the L929 cells, and the kill rates of composite scaffolds against E. coli and S. aureus after incubation for 24 h were 93.24 ± 1.22 % and 97.34 ± 0.23 %, respectively. Overall, the GDQ composite scaffolds have good mechanical properties adapted to skin bending, its good biocompatibility can promote the growth and migration of fibroblasts, reshape injured tissues, accelerate the wound healing, and excellent antimicrobial ability can inhibit the growth of E. coli and S. aureus, reducing the impact of bacterial infections on wounds. Moreover, the composite scaffolds have the potential to be used as exosom-loaded hydrogel dressings, which provides a basis for the subsequent research on the repair of diabetic foot ulcers.
Please use this identifier to cite or link to this item:
Download statistics for the last 12 months
Not enough data to produce graph