Monitoring the photosynthetic activity at single-cell level in Haematococcus lacustris

Patil, PP; Nagy, K; Abraham, A; Vass, I; Szabo, M

Monitoring the photosynthetic activity at single-cell level in Haematococcus lacustris

Patil, PP Nagy, K Abraham, A Vass, I Szabo, M

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Publisher:: Institute of Experimental Botany
Publication Type:: Journal Article
Citation:: PHOTOSYNTHETICA, 2023, 61, (4), pp. 473-482
Issue Date:: 2023-12-19

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Field	Value	Language
dc.contributor.author	Patil, PP
dc.contributor.author	Nagy, K
dc.contributor.author	Abraham, A
dc.contributor.author	Vass, I
dc.contributor.author	Szabo, M https://orcid.org/0000-0001-6235-2894
dc.date.accessioned	2024-05-16T20:11:49Z
dc.date.available	2024-05-16T20:11:49Z
dc.date.issued	2023-12-19
dc.identifier.citation	PHOTOSYNTHETICA, 2023, 61, (4), pp. 473-482
dc.identifier.issn	0300-3604
dc.identifier.issn	1573-9058
dc.identifier.uri	http://hdl.handle.net/10453/178998
dc.description.abstract	Haematococcus lacustris is an important species of green algae because it produces the high-value carotenoid astaxanthin. Astaxanthin production is enhanced by various stress conditions causing the transformation of green vegetative cells to red cells with high amounts of astaxanthin, which plays various photoprotective and antioxidant roles. Although intensive research has been conducted to reveal the regulation of astaxanthin production, the photosynthetic capacity of the various cell forms is unresolved at the single-cell level. In this work, we characterized the photosynthetic and morphological changes of Haematococcus cells, using a combination of microfluidic tools and microscopic chlorophyll fluorescence imaging. We found marked but reversible changes in the variable chlorophyll fluorescence signatures upon the transformation of green cells to red cells, and we propose that the photosynthetic activity as revealed by single-cell chlorophyll fluorescence kinetics serves as a useful phenotypic marker of the different cell forms of Haematococcus.
dc.language	en
dc.publisher	Institute of Experimental Botany
dc.relation.ispartof	PHOTOSYNTHETICA
dc.relation.isbasedon	10.32615/ps.2023.042
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0607 Plant Biology
dc.subject.classification	Plant Biology & Botany
dc.subject.classification	3108 Plant biology
dc.title	Monitoring the photosynthetic activity at single-cell level in Haematococcus lacustris
dc.type	Journal Article
utslib.citation.volume	61
utslib.for	0607 Plant Biology
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Science
utslib.copyright.status	open_access	*
dc.date.updated	2024-05-16T20:11:46Z
pubs.issue	4
pubs.publication-status	Published
pubs.volume	61
utslib.citation.issue	4

Abstract:

Haematococcus lacustris is an important species of green algae because it produces the high-value carotenoid astaxanthin. Astaxanthin production is enhanced by various stress conditions causing the transformation of green vegetative cells to red cells with high amounts of astaxanthin, which plays various photoprotective and antioxidant roles. Although intensive research has been conducted to reveal the regulation of astaxanthin production, the photosynthetic capacity of the various cell forms is unresolved at the single-cell level. In this work, we characterized the photosynthetic and morphological changes of Haematococcus cells, using a combination of microfluidic tools and microscopic chlorophyll fluorescence imaging. We found marked but reversible changes in the variable chlorophyll fluorescence signatures upon the transformation of green cells to red cells, and we propose that the photosynthetic activity as revealed by single-cell chlorophyll fluorescence kinetics serves as a useful phenotypic marker of the different cell forms of Haematococcus.

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http://hdl.handle.net/10453/178998