Extracellular vesicle formation in Euryarchaeota is driven by a small GTPase.

Mills, J; Gebhard, LJ; Schubotz, F; Shevchenko, A; Speth, DR; Liao, Y; Duggin, IG; Marchfelder, A; Erdmann, S

Extracellular vesicle formation in Euryarchaeota is driven by a small GTPase.

Mills, J Gebhard, LJ Schubotz, F Shevchenko, A Speth, DR Liao, Y

Duggin, IG Marchfelder, A Erdmann, S

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Publisher:: Proceedings of the National Academy of Sciences
Publication Type:: Journal Article
Citation:: Proc Natl Acad Sci U S A, 2024, 121, (10), pp. e2311321121
Issue Date:: 2024-03-05

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Field	Value	Language
dc.contributor.author	Mills, J
dc.contributor.author	Gebhard, LJ
dc.contributor.author	Schubotz, F
dc.contributor.author	Shevchenko, A
dc.contributor.author	Speth, DR
dc.contributor.author	Liao, Y https://orcid.org/0000-0002-8492-2648
dc.contributor.author	Duggin, IG
dc.contributor.author	Marchfelder, A
dc.contributor.author	Erdmann, S
dc.date.accessioned	2024-08-06T03:14:39Z
dc.date.available	2024-08-06T03:14:39Z
dc.date.issued	2024-03-05
dc.identifier.citation	Proc Natl Acad Sci U S A, 2024, 121, (10), pp. e2311321121
dc.identifier.issn	0027-8424
dc.identifier.issn	1091-6490
dc.identifier.uri	http://hdl.handle.net/10453/180099
dc.description.abstract	Since their discovery, extracellular vesicles (EVs) have changed our view on how organisms interact with their extracellular world. EVs are able to traffic a diverse array of molecules across different species and even domains, facilitating numerous functions. In this study, we investigate EV production in Euryarchaeota, using the model organism Haloferax volcanii. We uncover that EVs enclose RNA, with specific transcripts preferentially enriched, including those with regulatory potential, and conclude that EVs can act as an RNA communication system between haloarchaea. We demonstrate the key role of an EV-associated small GTPase for EV formation in H. volcanii that is also present across other diverse evolutionary branches of Archaea. We propose the name, ArvA, for the identified family of archaeal vesiculating GTPases. Additionally, we show that two genes in the same operon with arvA (arvB and arvC) are also involved in EV formation. Both, arvB and arvC, are closely associated with arvA in the majority of other archaea encoding ArvA. Our work demonstrates that small GTPases involved in membrane deformation and vesiculation, ubiquitous in Eukaryotes, are also present in Archaea and are widely distributed across diverse archaeal phyla.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Proceedings of the National Academy of Sciences
dc.relation.ispartof	Proc Natl Acad Sci U S A
dc.relation.isbasedon	10.1073/pnas.2311321121
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.mesh	Euryarchaeota
dc.subject.mesh	Monomeric GTP-Binding Proteins
dc.subject.mesh	Archaea
dc.subject.mesh	RNA
dc.subject.mesh	Haloferax volcanii
dc.subject.mesh	Extracellular Vesicles
dc.subject.mesh	Archaea
dc.subject.mesh	Euryarchaeota
dc.subject.mesh	Haloferax volcanii
dc.subject.mesh	Monomeric GTP-Binding Proteins
dc.subject.mesh	RNA
dc.subject.mesh	Extracellular Vesicles
dc.subject.mesh	Euryarchaeota
dc.subject.mesh	Monomeric GTP-Binding Proteins
dc.subject.mesh	Archaea
dc.subject.mesh	RNA
dc.subject.mesh	Haloferax volcanii
dc.subject.mesh	Extracellular Vesicles
dc.title	Extracellular vesicle formation in Euryarchaeota is driven by a small GTPase.
dc.type	Journal Article
utslib.citation.volume	121
utslib.location.activity	United States
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Science
pubs.organisational-group	University of Technology Sydney/Strength - AIMI - Australian Institute for Microbiology & Infection
utslib.copyright.status	open_access	*
dc.rights.license	This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0). To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/
dc.date.updated	2024-08-06T03:14:33Z
pubs.issue	10
pubs.publication-status	Published
pubs.volume	121
utslib.citation.issue	10

Abstract:

Since their discovery, extracellular vesicles (EVs) have changed our view on how organisms interact with their extracellular world. EVs are able to traffic a diverse array of molecules across different species and even domains, facilitating numerous functions. In this study, we investigate EV production in Euryarchaeota, using the model organism Haloferax volcanii. We uncover that EVs enclose RNA, with specific transcripts preferentially enriched, including those with regulatory potential, and conclude that EVs can act as an RNA communication system between haloarchaea. We demonstrate the key role of an EV-associated small GTPase for EV formation in H. volcanii that is also present across other diverse evolutionary branches of Archaea. We propose the name, ArvA, for the identified family of archaeal vesiculating GTPases. Additionally, we show that two genes in the same operon with arvA (arvB and arvC) are also involved in EV formation. Both, arvB and arvC, are closely associated with arvA in the majority of other archaea encoding ArvA. Our work demonstrates that small GTPases involved in membrane deformation and vesiculation, ubiquitous in Eukaryotes, are also present in Archaea and are widely distributed across diverse archaeal phyla.

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http://hdl.handle.net/10453/180099