Maternal third hand exposure to e-cigarette vapor alters lung and bone marrow immune cell responses in offspring in the absence or presence of flu infection

Donovan, C; Thorpe, AE; Yarak, R; Coward-Smith, M; Pillar, AL; Gomez, HM; Feng, M; Bai, X; Wang, M; Xenaki, D; Horvat, JC; Chen, H; Oliver, BGG; Kim, RY

Maternal third hand exposure to e-cigarette vapor alters lung and bone marrow immune cell responses in offspring in the absence or presence of flu infection

Thorpe, AE Yarak, R Coward-Smith, M Pillar, AL Gomez, HM Feng, M Bai, X Wang, M Xenaki, D Horvat, JC Chen, H Oliver, BGG Kim, RY

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Publisher:: American Physiological Society
Publication Type:: Journal Article
Citation:: American Journal of Physiology-Lung Cellular and Molecular Physiology

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Field	Value	Language
dc.contributor.author	Donovan, C https://orcid.org/0000-0003-4558-329X
dc.contributor.author	Thorpe, AE
dc.contributor.author	Yarak, R
dc.contributor.author	Coward-Smith, M
dc.contributor.author	Pillar, AL
dc.contributor.author	Gomez, HM
dc.contributor.author	Feng, M
dc.contributor.author	Bai, X
dc.contributor.author	Wang, M
dc.contributor.author	Xenaki, D
dc.contributor.author	Horvat, JC
dc.contributor.author	Chen, H
dc.contributor.author	Oliver, BGG
dc.contributor.author	Kim, RY
dc.date.accessioned	2024-10-01T05:21:53Z
dc.date.available	2024-10-01T05:21:53Z
dc.identifier.citation	American Journal of Physiology-Lung Cellular and Molecular Physiology
dc.identifier.issn	1040-0605
dc.identifier.issn	1522-1504
dc.identifier.uri	http://hdl.handle.net/10453/181124
dc.description.abstract	<jats:p> There is increasing evidence that third hand exposure to e-cigarette vapor (e-vapor) can have detrimental effects on the lungs. However, whether maternal exposure during pregnancy results in harmful changes to the offspring is unknown. Using two different e-cigarette settings (low versus high power), BALB/c mice were subjected to third hand e-vapor (e-vapor deposited onto towels, towels changed daily) in the absence or presence of nicotine, before, during, and after pregnancy. Male adult offspring were then infected with mouse-adapted influenza A virus (A/PR/8/34 H1N1) and lung and bone marrow immune cell responses assessed 7 days post infection. Maternal third hand exposure to low power (<jats:sub>M</jats:sub>LP) or high power (<jats:sub>M</jats:sub>HP) e-vapor with nicotine (<jats:sub>M</jats:sub>LP+NIC and <jats:sub>M</jats:sub>HP+NIC, respectively) increased the percentage of lung immune cells and neutrophils in the bone marrow. Interestingly, Flu-infected offspring from <jats:sub>M</jats:sub>LP+NIC and <jats:sub>M</jats:sub>HP+NIC groups had lower percentages of lung alveolar macrophages, and more pronounced increases in neutrophils in the bone marrow, when compared to offspring from <jats:sub>M</jats:sub>Sham Flu controls. Flu infection also decreased the percentage of lung CD4+ T cells and increased the percentage of lung CD8+ T cells, irrespective of maternal exposure (<jats:sub>M</jats:sub>LP−/+NIC and <jats:sub>M</jats:sub>HP−/+NIC). Significantly, both <jats:sub>M</jats:sub>LP+NIC and <jats:sub>M</jats:sub>HP+NIC resulted in blunted activation of lung CD4+ T cells, but only <jats:sub>M</jats:sub>LP+NIC caused blunted activation of lung CD8+ T cells. Together, we show for the first time that maternal third hand exposure to e-vapor results in significant, long-lived effects on lung and bone marrow immune cell responses in offspring at baseline and in response to Flu infection. </jats:p>
dc.language	en
dc.publisher	American Physiological Society
dc.relation.ispartof	American Journal of Physiology-Lung Cellular and Molecular Physiology
dc.relation.isbasedon	10.1152/ajplung.00078.2024
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0606 Physiology, 1116 Medical Physiology
dc.subject.classification	Respiratory System
dc.subject.classification	3201 Cardiovascular medicine and haematology
dc.subject.classification	3208 Medical physiology
dc.title	Maternal third hand exposure to e-cigarette vapor alters lung and bone marrow immune cell responses in offspring in the absence or presence of flu infection
dc.type	Journal Article
utslib.for	0606 Physiology
utslib.for	1116 Medical Physiology
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Science
pubs.organisational-group	University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access	*
dc.date.updated	2024-10-01T05:21:50Z
pubs.publication-status	Published online

Abstract:

There is increasing evidence that third hand exposure to e-cigarette vapor (e-vapor) can have detrimental effects on the lungs. However, whether maternal exposure during pregnancy results in harmful changes to the offspring is unknown. Using two different e-cigarette settings (low versus high power), BALB/c mice were subjected to third hand e-vapor (e-vapor deposited onto towels, towels changed daily) in the absence or presence of nicotine, before, during, and after pregnancy. Male adult offspring were then infected with mouse-adapted influenza A virus (A/PR/8/34 H1N1) and lung and bone marrow immune cell responses assessed 7 days post infection. Maternal third hand exposure to low power (MLP) or high power (MHP) e-vapor with nicotine (MLP+NIC and MHP+NIC, respectively) increased the percentage of lung immune cells and neutrophils in the bone marrow. Interestingly, Flu-infected offspring from MLP+NIC and MHP+NIC groups had lower percentages of lung alveolar macrophages, and more pronounced increases in neutrophils in the bone marrow, when compared to offspring from MSham Flu controls. Flu infection also decreased the percentage of lung CD4+ T cells and increased the percentage of lung CD8+ T cells, irrespective of maternal exposure (MLP−/+NIC and MHP−/+NIC). Significantly, both MLP+NIC and MHP+NIC resulted in blunted activation of lung CD4+ T cells, but only MLP+NIC caused blunted activation of lung CD8+ T cells. Together, we show for the first time that maternal third hand exposure to e-vapor results in significant, long-lived effects on lung and bone marrow immune cell responses in offspring at baseline and in response to Flu infection.

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http://hdl.handle.net/10453/181124