Imaging of surface O<inf>2</inf>dynamics in corals with magnetic micro optode particles

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Journal Article
Marine Biology, 2012, 159 (7), pp. 1621 - 1631
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We present a new method for quantifying spatio-temporal O2distribution and dynamics at biologically active surfaces with a complex surface topography. Magnetized O2optode microparticles (~80-100 μm) containing the NIR-emitting luminophore platinum (II) meso-tetra(4-fluorophenyl) tetrabenzoporphyrin (PtTPTBPF; ex. max. 615 nm; em. max. 780 nm) were distributed across the surface tissue of the scleractinian coral Caulastrea furcata and were held in place with a strong magnet. The O2-dependent luminescence of the particles was mapped with a lifetime imaging system enabling measurements of the lateral surface heterogeneity of the O2microenvironment across coral polyps exposed to flow. Mapping steady-state O2concentrations under constant light and O2dynamics during experimental light-dark shifts enabled us to identify zones of different photosynthetic activities within a single coral polyp linked to the distribution of coral host pigments. Measurements under increasing irradiance showed typical saturation curves of O2concentration and estimates of gross photosynthesis that could be spatially resolved at ~100 μm pixel resolution. The new method for O2imaging with magnetized optode particles has much potential to be used in studies of the surface microenvironment of other aquatic systems such as sediments, biofilms, plant, and animal tissue. © 2012 Springer-Verlag.
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