Superhydrophobic, nanotextured polyvinyl chloride films for delaying Pseudomonas aeruginosa attachment to intubation tubes and medical plastics

Loo, CY; Young, PM; Lee, WH; Cavaliere, R; Whitchurch, CB; Rohanizadeh, R

Superhydrophobic, nanotextured polyvinyl chloride films for delaying Pseudomonas aeruginosa attachment to intubation tubes and medical plastics

Loo, CY Young, PM

Lee, WH Cavaliere, R

Whitchurch, CB

Rohanizadeh, R

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Publication Type:: Journal Article
Citation:: Acta Biomaterialia, 2012, 8 (5), pp. 1881 - 1890
Issue Date:: 2012-05-01

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Field	Value	Language
dc.contributor.author	Loo, CY	en_US
dc.contributor.author	Young, PM https://orcid.org/0000-0002-4357-7999	en_US
dc.contributor.author	Lee, WH	en_US
dc.contributor.author	Cavaliere, R https://orcid.org/0000-0002-4709-5081	en_US
dc.contributor.author	Whitchurch, CB https://orcid.org/0000-0003-2296-3791	en_US
dc.contributor.author	Rohanizadeh, R	en_US
dc.date.available	2012-01-10	en_US
dc.date.issued	2012-05-01	en_US
dc.identifier.citation	Acta Biomaterialia, 2012, 8 (5), pp. 1881 - 1890	en_US
dc.identifier.issn	1742-7061	en_US
dc.identifier.uri	http://hdl.handle.net/10453/18121
dc.description.abstract	Bacterial attachment onto the surface of polymers in medical devices such as polyvinyl chloride (PVC) is influenced by the physicochemical properties of the polymer, including its surface hydrophobicity and roughness. In this study, to prevent biofilm formation onto PVC devices, the PVC surface was modified using a combination of solvent (tetrahydrofuran) and non-solvents (i.e. ethanol and methanol). The surface of unmodified PVC was smooth and relatively hydrophobic (water contact angle (CA) = 80°). Ethanol-treated PVCs revealed the presence of micron-sized particulates and porous structures as the concentration of ethanol was increased. Surface hydrophobicity (measured in terms of CA) increased from 73° to 150° as the ethanol concentration increased from 15% to 35% (v/v). In general, methanol-treated PVCs were more hydrophilic compared to those treated with ethanol. The colonization of Pseudomonas aeruginosa PAO1 onto unmodified PVC surface was rapid, and individual bacterial cells could be seen after 6 h incubation. On the surface of treated PVC, the secretion of extracellular matrix layers was evident at 18 h and P. aeruginosa PAO1 start to form microcolonies at 24 h of incubation. The initial attachment of P. aeruginosa PAO1 was delayed to 18 and 24 h, respectively in the PVCs treated with 25% (v/v) and 35% (v/v) ethanol. It can be concluded that the treatment used in this study to prepare superhydrophobic PVC surface prevented the colonization of bacteria up to 24 h after culture. © 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.	en_US
dc.relation.ispartof	Acta Biomaterialia	en_US
dc.relation.isbasedon	10.1016/j.actbio.2012.01.015	en_US
dc.subject.classification	Biomedical Engineering	en_US
dc.subject.mesh	Pseudomonas aeruginosa	en_US
dc.subject.mesh	Polyvinyl Chloride	en_US
dc.subject.mesh	Plastics	en_US
dc.subject.mesh	Coated Materials, Biocompatible	en_US
dc.subject.mesh	Membranes, Artificial	en_US
dc.subject.mesh	Intubation, Intratracheal	en_US
dc.subject.mesh	Materials Testing	en_US
dc.subject.mesh	Bacterial Adhesion	en_US
dc.subject.mesh	Hydrophobic and Hydrophilic Interactions	en_US
dc.title	Superhydrophobic, nanotextured polyvinyl chloride films for delaying Pseudomonas aeruginosa attachment to intubation tubes and medical plastics	en_US
dc.type	Journal Article
utslib.citation.volume	5	en_US
utslib.citation.volume	8	en_US
utslib.for	0605 Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	5	en_US
pubs.publication-status	Published	en_US
pubs.volume	8	en_US

Abstract:

Bacterial attachment onto the surface of polymers in medical devices such as polyvinyl chloride (PVC) is influenced by the physicochemical properties of the polymer, including its surface hydrophobicity and roughness. In this study, to prevent biofilm formation onto PVC devices, the PVC surface was modified using a combination of solvent (tetrahydrofuran) and non-solvents (i.e. ethanol and methanol). The surface of unmodified PVC was smooth and relatively hydrophobic (water contact angle (CA) = 80°). Ethanol-treated PVCs revealed the presence of micron-sized particulates and porous structures as the concentration of ethanol was increased. Surface hydrophobicity (measured in terms of CA) increased from 73° to 150° as the ethanol concentration increased from 15% to 35% (v/v). In general, methanol-treated PVCs were more hydrophilic compared to those treated with ethanol. The colonization of Pseudomonas aeruginosa PAO1 onto unmodified PVC surface was rapid, and individual bacterial cells could be seen after 6 h incubation. On the surface of treated PVC, the secretion of extracellular matrix layers was evident at 18 h and P. aeruginosa PAO1 start to form microcolonies at 24 h of incubation. The initial attachment of P. aeruginosa PAO1 was delayed to 18 and 24 h, respectively in the PVCs treated with 25% (v/v) and 35% (v/v) ethanol. It can be concluded that the treatment used in this study to prepare superhydrophobic PVC surface prevented the colonization of bacteria up to 24 h after culture. © 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

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http://hdl.handle.net/10453/18121