Mouse guanylate-binding proteins of the chromosome 3 cluster do not mediate antiviral activity in vitro or in mouse models of infection.

Tessema, MB; Feng, S; Enosi Tuipulotu, D; Farrukee, R; Ngo, C; Gago da Graça, C; Yamomoto, M; Utzschneider, DT; Brooks, AG; Londrigan, SL; Man, SM; Reading, PC

Mouse guanylate-binding proteins of the chromosome 3 cluster do not mediate antiviral activity in vitro or in mouse models of infection.

Tessema, MB Feng, S Enosi Tuipulotu, D

Farrukee, R Ngo, C Gago da Graça, C Yamomoto, M Utzschneider, DT Brooks, AG Londrigan, SL Man, SM Reading, PC

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Publisher:: NATURE PORTFOLIO
Publication Type:: Journal Article
Citation:: Commun Biol, 2024, 7, (1), pp. 1050
Issue Date:: 2024-08-25

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Field	Value	Language
dc.contributor.author	Tessema, MB
dc.contributor.author	Feng, S
dc.contributor.author	Enosi Tuipulotu, D https://orcid.org/0000-0002-6442-4633
dc.contributor.author	Farrukee, R
dc.contributor.author	Ngo, C
dc.contributor.author	Gago da Graça, C
dc.contributor.author	Yamomoto, M
dc.contributor.author	Utzschneider, DT
dc.contributor.author	Brooks, AG
dc.contributor.author	Londrigan, SL
dc.contributor.author	Man, SM
dc.contributor.author	Reading, PC
dc.date.accessioned	2024-10-09T01:12:46Z
dc.date.available	2024-08-16
dc.date.available	2024-10-09T01:12:46Z
dc.date.issued	2024-08-25
dc.identifier.citation	Commun Biol, 2024, 7, (1), pp. 1050
dc.identifier.issn	2399-3642
dc.identifier.issn	2399-3642
dc.identifier.uri	http://hdl.handle.net/10453/181291
dc.description.abstract	Dynamin-like GTPase proteins, including myxoma (Mx) and guanylate-binding proteins (GBPs), are among the many interferon stimulated genes induced following viral infections. While studies report that human (h)GBPs inhibit different viruses in vitro, few have convincingly demonstrated that mouse (m)GBPs mediate antiviral activity, although mGBP-deficient mice have been used extensively to define their importance in immunity to diverse intracellular bacteria and protozoa. Herein, we demonstrate that individual (overexpression) or collective (knockout (KO) mice) mGBPs of the chromosome 3 cluster (mGBPchr3) do not inhibit replication of five viruses from different virus families in vitro, nor do we observe differences in virus titres recovered from wild type versus mGBPchr3 KO mice after infection with three of these viruses (influenza A virus, herpes simplex virus type 1 or lymphocytic choriomeningitis virus). These data indicate that mGBPchr3 do not appear to be a major component of cell-intrinsic antiviral immunity against the diverse viruses tested in our studies.
dc.format	Electronic
dc.language	eng
dc.publisher	NATURE PORTFOLIO
dc.relation.ispartof	Commun Biol
dc.relation.isbasedon	10.1038/s42003-024-06748-8
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.classification	31 Biological sciences
dc.subject.classification	32 Biomedical and clinical sciences
dc.subject.mesh	Animals
dc.subject.mesh	Mice
dc.subject.mesh	GTP-Binding Proteins
dc.subject.mesh	Mice, Knockout
dc.subject.mesh	Disease Models, Animal
dc.subject.mesh	Virus Replication
dc.subject.mesh	Herpesvirus 1, Human
dc.subject.mesh	Mice, Inbred C57BL
dc.subject.mesh	Lymphocytic choriomeningitis virus
dc.subject.mesh	Virus Diseases
dc.subject.mesh	Animals
dc.subject.mesh	Mice, Inbred C57BL
dc.subject.mesh	Mice, Knockout
dc.subject.mesh	Mice
dc.subject.mesh	Herpesvirus 1, Human
dc.subject.mesh	Lymphocytic choriomeningitis virus
dc.subject.mesh	Virus Diseases
dc.subject.mesh	Disease Models, Animal
dc.subject.mesh	GTP-Binding Proteins
dc.subject.mesh	Virus Replication
dc.subject.mesh	Animals
dc.subject.mesh	Mice
dc.subject.mesh	GTP-Binding Proteins
dc.subject.mesh	Mice, Knockout
dc.subject.mesh	Disease Models, Animal
dc.subject.mesh	Virus Replication
dc.subject.mesh	Herpesvirus 1, Human
dc.subject.mesh	Mice, Inbred C57BL
dc.subject.mesh	Lymphocytic choriomeningitis virus
dc.subject.mesh	Virus Diseases
dc.title	Mouse guanylate-binding proteins of the chromosome 3 cluster do not mediate antiviral activity in vitro or in mouse models of infection.
dc.type	Journal Article
utslib.citation.volume	7
utslib.location.activity	England
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Science
pubs.organisational-group	University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access	*
dc.rights.license	This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND 4.0). To view a copy of this license, visit https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.date.updated	2024-10-09T01:12:39Z
pubs.issue	1
pubs.publication-status	Published online
pubs.volume	7
utslib.citation.issue	1

Abstract:

Dynamin-like GTPase proteins, including myxoma (Mx) and guanylate-binding proteins (GBPs), are among the many interferon stimulated genes induced following viral infections. While studies report that human (h)GBPs inhibit different viruses in vitro, few have convincingly demonstrated that mouse (m)GBPs mediate antiviral activity, although mGBP-deficient mice have been used extensively to define their importance in immunity to diverse intracellular bacteria and protozoa. Herein, we demonstrate that individual (overexpression) or collective (knockout (KO) mice) mGBPs of the chromosome 3 cluster (mGBPchr3) do not inhibit replication of five viruses from different virus families in vitro, nor do we observe differences in virus titres recovered from wild type versus mGBPchr3 KO mice after infection with three of these viruses (influenza A virus, herpes simplex virus type 1 or lymphocytic choriomeningitis virus). These data indicate that mGBPchr3 do not appear to be a major component of cell-intrinsic antiviral immunity against the diverse viruses tested in our studies.

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http://hdl.handle.net/10453/181291