Population structure and genetic diversity of the rare and endangered Sinocalamus mucclure and Markhamia stipulata in ba be national park, Vietnam

van Hung, H; de Filippis, L; Buckney, R

Population structure and genetic diversity of the rare and endangered Sinocalamus mucclure and Markhamia stipulata in ba be national park, Vietnam

van Hung, H de Filippis, L Buckney, R

Permalink

Publication Type:: Journal Article
Citation:: Asian Journal of Plant Sciences, 2011, 10 (6), pp. 312 - 322
Issue Date:: 2011-12-13

Closed Access

	Filename	Description	Size
	2010005857OK.pdf		935.08 kB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	van Hung, H	en_US
dc.contributor.author	de Filippis, L	en_US
dc.contributor.author	Buckney, R	en_US
dc.date.issued	2011-12-13	en_US
dc.identifier.citation	Asian Journal of Plant Sciences, 2011, 10 (6), pp. 312 - 322	en_US
dc.identifier.issn	1682-3974	en_US
dc.identifier.uri	http://hdl.handle.net/10453/18149
dc.description.abstract	Plant species composition in Ba Be National Park, Vietnam identified five plants that were rare and endangered and at serious risk of disappearing from the park. However, only Sinocalamus mucclure (string bamboo) and Markhamia stipulata had sufficient numbers to warrant molecular analysis. ISSR (Inter Simple Sequence Repeats) and RAPD-PCR (Random Amplified Polymorphic DNA-Polymerase Chain Reaction) were used to determine genetic differences between populations of Sinocalamus mucclure and Markhamia stipulata and whether genetic diversity was correlated to geographic distance. Disturbance in the area was evident, however, biogeography features usually associated with human disturbance was a minor contribution to species fragmentation. Analysis of similarity in biogeography and vegetation parameters demonstrated that similarity between the sites was between 65 and 85% and introduced plant species to the park was low. Trees dominated the canopy and species richness varied between the sites. Multi-Dimensional Scaling (MDS) of molecular data showed significant differences in populations of both plants and that substantial genetic variation between individuals of both species were present. ISSR revealed slightly less genetic diversity in both species (70-71% polymorphism) than RAPD-PCR (65-71% polymorphism). In conclusion, ISSR appeared to be more discriminatoiy than RAPD-PCR but both were versatile, sensitive and cost effective methods for use in genetic diversity and conservation genetics. © 2011 Asian Network for Scientific Information.	en_US
dc.relation.ispartof	Asian Journal of Plant Sciences	en_US
dc.relation.isbasedon	10.3923/ajps.2011.312.322	en_US
dc.subject.classification	Plant Biology & Botany	en_US
dc.title	Population structure and genetic diversity of the rare and endangered Sinocalamus mucclure and Markhamia stipulata in ba be national park, Vietnam	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.citation.volume	10	en_US
utslib.for	0607 Plant Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	6	en_US
pubs.publication-status	Published	en_US
pubs.volume	10	en_US

Abstract:

Plant species composition in Ba Be National Park, Vietnam identified five plants that were rare and endangered and at serious risk of disappearing from the park. However, only Sinocalamus mucclure (string bamboo) and Markhamia stipulata had sufficient numbers to warrant molecular analysis. ISSR (Inter Simple Sequence Repeats) and RAPD-PCR (Random Amplified Polymorphic DNA-Polymerase Chain Reaction) were used to determine genetic differences between populations of Sinocalamus mucclure and Markhamia stipulata and whether genetic diversity was correlated to geographic distance. Disturbance in the area was evident, however, biogeography features usually associated with human disturbance was a minor contribution to species fragmentation. Analysis of similarity in biogeography and vegetation parameters demonstrated that similarity between the sites was between 65 and 85% and introduced plant species to the park was low. Trees dominated the canopy and species richness varied between the sites. Multi-Dimensional Scaling (MDS) of molecular data showed significant differences in populations of both plants and that substantial genetic variation between individuals of both species were present. ISSR revealed slightly less genetic diversity in both species (70-71% polymorphism) than RAPD-PCR (65-71% polymorphism). In conclusion, ISSR appeared to be more discriminatoiy than RAPD-PCR but both were versatile, sensitive and cost effective methods for use in genetic diversity and conservation genetics. © 2011 Asian Network for Scientific Information.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/18149