Regulation of antigen processing and presentation molecules in West Nile virus-infected human skin fibroblasts

Arnold, SJ; Osvath, SR; Hall, RA; King, NJC; Sedger, LM

Regulation of antigen processing and presentation molecules in West Nile virus-infected human skin fibroblasts

Arnold, SJ Osvath, SR Hall, RA King, NJC Sedger, LM

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Publication Type:: Journal Article
Citation:: Virology, 2004, 324 (2), pp. 286 - 296
Issue Date:: 2004-07-01

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Field	Value	Language
dc.contributor.author	Arnold, SJ	en_US
dc.contributor.author	Osvath, SR	en_US
dc.contributor.author	Hall, RA	en_US
dc.contributor.author	King, NJC	en_US
dc.contributor.author	Sedger, LM https://orcid.org/0000-0003-1009-5683	en_US
dc.date.available	2004-03-29	en_US
dc.date.issued	2004-07-01	en_US
dc.identifier.citation	Virology, 2004, 324 (2), pp. 286 - 296	en_US
dc.identifier.issn	0042-6822	en_US
dc.identifier.uri	http://hdl.handle.net/10453/18506
dc.description.abstract	Infection of humans with the West Nile flavivirus principally occurs via tick and mosquito bites. Here, we document the expression of antigen processing and presentation molecules in West Nile virus (WNV)-infected human skin fibroblast (HFF) cells. Using a new Flavivirus-specific antibody, 4G4, we have analyzed cell surface human leukocyte antigen (HLA) expression on virus-infected cells at a single cell level. Using this approach, we show that West Nile Virus infection alters surface HLA expression on both infected HFF and neighboring uninfected HFF cells. Interestingly, increased surface HLA evident on infected HFF cultures is almost entirely due to virus-induced interferon (IFN)α/β because IFNα/β-neutralizing antibodies completely prevent increased surface HLA expression. In contrast, RT-PCR analysis indicates that WNV infection results in increased mRNAs for HLA-A, -B, and -C genes, and HLA-associated molecules low molecular weight polypeptide-2 (LMP-2) and transporter associated with antigen presentation-1 (TAP-1), but induction of these mRNAs is not diminished in HFF cells cultured with IFNα/β- neutralizing antibodies. Taken together, these data support the idea that that both cytokine-dependent and cytokine-independent mechanisms account for WNV-induced HLA expression in human skin fibroblasts. © 2004 Elsevier Inc. All rights reserved.	en_US
dc.relation.ispartof	Virology	en_US
dc.relation.isbasedon	10.1016/j.virol.2004.03.036	en_US
dc.subject.classification	Virology	en_US
dc.subject.mesh	Antigen-Presenting Cells	en_US
dc.subject.mesh	Fibroblasts	en_US
dc.subject.mesh	Skin	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	West Nile virus	en_US
dc.subject.mesh	West Nile Fever	en_US
dc.subject.mesh	Fluoresceins	en_US
dc.subject.mesh	Cysteine Endopeptidases	en_US
dc.subject.mesh	Tumor Necrosis Factor-alpha	en_US
dc.subject.mesh	ATP-Binding Cassette Transporters	en_US
dc.subject.mesh	Interferon-beta	en_US
dc.subject.mesh	Viral Nonstructural Proteins	en_US
dc.subject.mesh	RNA, Messenger	en_US
dc.subject.mesh	Antibodies, Monoclonal	en_US
dc.subject.mesh	HLA-A Antigens	en_US
dc.subject.mesh	HLA Antigens	en_US
dc.subject.mesh	Fluorescent Antibody Technique	en_US
dc.subject.mesh	Immunohistochemistry	en_US
dc.subject.mesh	Reverse Transcriptase Polymerase Chain Reaction	en_US
dc.subject.mesh	ATP-Binding Cassette Sub-Family B Member 2	en_US
dc.subject.mesh	ATP Binding Cassette Transporter, Subfamily B, Member 2	en_US
dc.title	Regulation of antigen processing and presentation molecules in West Nile virus-infected human skin fibroblasts	en_US
dc.type	Journal Article
utslib.citation.volume	2	en_US
utslib.citation.volume	324	en_US
utslib.for	1107 Immunology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	2	en_US
pubs.publication-status	Published	en_US
pubs.volume	324	en_US

Abstract:

Infection of humans with the West Nile flavivirus principally occurs via tick and mosquito bites. Here, we document the expression of antigen processing and presentation molecules in West Nile virus (WNV)-infected human skin fibroblast (HFF) cells. Using a new Flavivirus-specific antibody, 4G4, we have analyzed cell surface human leukocyte antigen (HLA) expression on virus-infected cells at a single cell level. Using this approach, we show that West Nile Virus infection alters surface HLA expression on both infected HFF and neighboring uninfected HFF cells. Interestingly, increased surface HLA evident on infected HFF cultures is almost entirely due to virus-induced interferon (IFN)α/β because IFNα/β-neutralizing antibodies completely prevent increased surface HLA expression. In contrast, RT-PCR analysis indicates that WNV infection results in increased mRNAs for HLA-A, -B, and -C genes, and HLA-associated molecules low molecular weight polypeptide-2 (LMP-2) and transporter associated with antigen presentation-1 (TAP-1), but induction of these mRNAs is not diminished in HFF cells cultured with IFNα/β- neutralizing antibodies. Taken together, these data support the idea that that both cytokine-dependent and cytokine-independent mechanisms account for WNV-induced HLA expression in human skin fibroblasts. © 2004 Elsevier Inc. All rights reserved.

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http://hdl.handle.net/10453/18506