Cross-protection of chicken immunoglobulin Y antibodies against H5N1 and H1N1 viruses passively administered in mice

Wallach, MG; Webby, RJ; Islam, F; Walkden-Brown, S; Emmoth, E; Feinstein, R; Gronvik, KO

Cross-protection of chicken immunoglobulin Y antibodies against H5N1 and H1N1 viruses passively administered in mice

Wallach, MG Webby, RJ Islam, F Walkden-Brown, S Emmoth, E Feinstein, R Gronvik, KO

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Publication Type:: Journal Article
Citation:: Clinical and Vaccine Immunology, 2011, 18 (7), pp. 1083 - 1090
Issue Date:: 2011-07-01

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Field	Value	Language
dc.contributor.author	Wallach, MG	en_US
dc.contributor.author	Webby, RJ	en_US
dc.contributor.author	Islam, F	en_US
dc.contributor.author	Walkden-Brown, S	en_US
dc.contributor.author	Emmoth, E	en_US
dc.contributor.author	Feinstein, R	en_US
dc.contributor.author	Gronvik, KO	en_US
dc.date.issued	2011-07-01	en_US
dc.identifier.citation	Clinical and Vaccine Immunology, 2011, 18 (7), pp. 1083 - 1090	en_US
dc.identifier.issn	1556-6811	en_US
dc.identifier.uri	http://hdl.handle.net/10453/18520
dc.description.abstract	Influenza viruses remain a major threat to global health due to their ability to undergo change through antigenic drift and antigenic shift. We postulated that avian IgY antibodies represent a low-cost, effective, and well-tolerated approach that can easily be scaled up to produce enormous quantities of protective antibodies. These IgY antibodies can be administered passively in humans (orally and intranasally) and can be used quickly and safely to help in the fight against an influenza pandemic. In this study, we raised IgY antibodies against H1N1, H3N2, and H5N1 influenza viruses. We demonstrated that, using whole inactivated viruses alone and in combination to immunize hens, we were able to induce a high level of anti-influenza virus IgY in the sera and eggs, which lasted for at least 2 months after two immunizations. Furthermore, we found that by use of in vitro assays to test for the ability of IgY to inhibit hemagglutination (HI test) and virus infectivity (serum neutralization test), IgYs inhibited the homologous as well as in some cases heterologous clades and strains of viruses. Using an in vivo mouse model system, we found that, when administered intranasally 1 h prior to infection, IgY to H5N1 protected 100% of the mice against lethal challenge with H5N1. Of particular interest was the finding that IgY to H5N1 cross-protected against A/Puerto Rico/8/34 (H1N1) both in vitro and in vivo. Based on our results, we conclude that anti-influenza virus IgY can be used to help prevent influenza virus infection. Copyright © 2011, American Society for Microbiology. All Rights Reserved.	en_US
dc.relation.ispartof	Clinical and Vaccine Immunology	en_US
dc.relation.isbasedon	10.1128/CVI.05075-11	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.classification	Immunology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Chickens	en_US
dc.subject.mesh	Mice	en_US
dc.subject.mesh	Immunoglobulins	en_US
dc.subject.mesh	Influenza Vaccines	en_US
dc.subject.mesh	Treatment Outcome	en_US
dc.subject.mesh	Administration, Intranasal	en_US
dc.subject.mesh	Influenza A Virus, H5N1 Subtype	en_US
dc.subject.mesh	Influenza A Virus, H1N1 Subtype	en_US
dc.subject.mesh	Cross Protection	en_US
dc.title	Cross-protection of chicken immunoglobulin Y antibodies against H5N1 and H1N1 viruses passively administered in mice	en_US
dc.type	Journal Article
utslib.citation.volume	7	en_US
utslib.citation.volume	18	en_US
utslib.for	1107 Immunology	en_US
dc.location.activity	WOS:000292378200007	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	7	en_US
pubs.publication-status	Published	en_US
pubs.volume	18	en_US

Abstract:

Influenza viruses remain a major threat to global health due to their ability to undergo change through antigenic drift and antigenic shift. We postulated that avian IgY antibodies represent a low-cost, effective, and well-tolerated approach that can easily be scaled up to produce enormous quantities of protective antibodies. These IgY antibodies can be administered passively in humans (orally and intranasally) and can be used quickly and safely to help in the fight against an influenza pandemic. In this study, we raised IgY antibodies against H1N1, H3N2, and H5N1 influenza viruses. We demonstrated that, using whole inactivated viruses alone and in combination to immunize hens, we were able to induce a high level of anti-influenza virus IgY in the sera and eggs, which lasted for at least 2 months after two immunizations. Furthermore, we found that by use of in vitro assays to test for the ability of IgY to inhibit hemagglutination (HI test) and virus infectivity (serum neutralization test), IgYs inhibited the homologous as well as in some cases heterologous clades and strains of viruses. Using an in vivo mouse model system, we found that, when administered intranasally 1 h prior to infection, IgY to H5N1 protected 100% of the mice against lethal challenge with H5N1. Of particular interest was the finding that IgY to H5N1 cross-protected against A/Puerto Rico/8/34 (H1N1) both in vitro and in vivo. Based on our results, we conclude that anti-influenza virus IgY can be used to help prevent influenza virus infection. Copyright © 2011, American Society for Microbiology. All Rights Reserved.

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http://hdl.handle.net/10453/18520