Comparison of Microscopy, Culture, and Conventional Polymerase Chain Reaction for Detection of Blastocystis sp. in Clinical Stool Samples.

Publisher:
American Society of Tropical Medicine and Hygiene
Publication Type:
Journal Article
Citation:
American Journal of Tropical Medicine and Hygiene, 2011, 84 (2S), pp. 308 - 312
Issue Date:
2011-01
Full metadata record
Files in This Item:
Filename Description Size
Thumbnail2009007264OK.pdf432.59 kB
Adobe PDF
We tested 513 stool samples from patients in Sydney, Australia for Blastocystis by using five diagnostic techniques: microscopy of a permanently stained smear using a modified iron-hematoxylin stain, two xenic culture systems (a modified Boeck and Drbohlav's medium and tryptone, yeast extract, glucose, methionine-9 medium), and two published conventional polymerase chain reaction methods specific for the small subunit ribosomal DNA. Ninety-eight (19%) samples were positive for Blastocystis in one or more of the diagnostic techniques. The PCR 2 method was the most sensitive at detecting Blastocystis with a sensitivity of 94%, and the least sensitive was microscopy of the permanent stain (48%). Subtype 3 was the most predominant subtype (present in 43% of samples assigned to this group). This study highlights the low sensitivity of microscopy when used as the sole diagnostic modality for detection of Blastocystis sp
Please use this identifier to cite or link to this item: