| Field |
Value |
Language |
|
dc.contributor.author |
Su, Y |
|
|
dc.contributor.author |
He, Z |
|
|
dc.contributor.author |
Li, J
https://orcid.org/0000-0002-3584-6765
|
|
|
dc.contributor.author |
Chen, Q |
|
|
dc.contributor.author |
Wang, D |
|
|
dc.contributor.author |
Yang, Z |
|
|
dc.contributor.author |
Yuan, Y |
|
|
dc.contributor.author |
Chen, L |
|
|
dc.contributor.author |
Ye, F |
|
|
dc.contributor.author |
Xing, D |
|
|
dc.contributor.author |
Li, H |
|
|
dc.contributor.author |
Lin, J |
|
|
dc.date.accessioned |
2026-02-03T09:04:59Z |
|
|
dc.date.available |
2025-10-03 |
|
|
dc.date.available |
2026-02-03T09:04:59Z |
|
|
dc.date.issued |
2025-11-18 |
|
|
dc.identifier.citation |
Stem Cell Res Ther, 2025, 16, (1), pp. 647 |
|
|
dc.identifier.issn |
1757-6512 |
|
|
dc.identifier.issn |
1757-6512 |
|
|
dc.identifier.uri |
http://hdl.handle.net/10453/192868
|
|
|
dc.description.abstract |
BACKGROUND: Endochondral ossification (ECO) is essential for bone regeneration, involving cartilage formation, hypertrophy, angiogenesis, and ossification. Co-culturing mesenchymal stem cells (MSCs) with endothelial cells (ECs) shows potential to enhance bone regeneration but has not been effectively applied to ECO strategy. METHODS: We examined the synergistic effects of MSCs and ECs on chondrogenesis, osteogenesis, and angiogenesis, followed by transcriptomic analysis. ECO organoids were formed in the scaffolds, and a critical-sized calvarial bone defect model was used for in vivo evaluation. RESULTS: Co-culture of ECs and MSCs promoted osteogenic differentiation and hypertrophic chondrogenic differentiation of MSCs. The ECO organoids exhibited enhanced vascularization and improved mineralization. In vivo, the co-culture group showed superior vascularization and bone repair compared to the MSCs-only group. CONCLUSIONS: Co-culturing ECs with MSCs in ECO organoids enhances bone regeneration, offering a promising alternative to traditional tissue engineering strategies. This approach may improve therapeutic outcomes by promoting endochondral bone formation. |
|
|
dc.format |
Electronic |
|
|
dc.language |
eng |
|
|
dc.publisher |
BMC |
|
|
dc.relation.ispartof |
Stem Cell Res Ther |
|
|
dc.relation.isbasedon |
10.1186/s13287-025-04733-4 |
|
|
dc.rights |
info:eu-repo/semantics/openAccess |
|
|
dc.subject |
06 Biological Sciences, 10 Technology, 11 Medical and Health Sciences |
|
|
dc.subject.classification |
31 Biological sciences |
|
|
dc.subject.mesh |
Mesenchymal Stem Cells |
|
|
dc.subject.mesh |
Bone Regeneration |
|
|
dc.subject.mesh |
Osteogenesis |
|
|
dc.subject.mesh |
Coculture Techniques |
|
|
dc.subject.mesh |
Animals |
|
|
dc.subject.mesh |
Organoids |
|
|
dc.subject.mesh |
Endothelial Cells |
|
|
dc.subject.mesh |
Cell Differentiation |
|
|
dc.subject.mesh |
Humans |
|
|
dc.subject.mesh |
Chondrogenesis |
|
|
dc.subject.mesh |
Mice |
|
|
dc.subject.mesh |
Tissue Engineering |
|
|
dc.subject.mesh |
Neovascularization, Physiologic |
|
|
dc.subject.mesh |
Organoids |
|
|
dc.subject.mesh |
Endothelial Cells |
|
|
dc.subject.mesh |
Mesenchymal Stem Cells |
|
|
dc.subject.mesh |
Animals |
|
|
dc.subject.mesh |
Humans |
|
|
dc.subject.mesh |
Mice |
|
|
dc.subject.mesh |
Coculture Techniques |
|
|
dc.subject.mesh |
Tissue Engineering |
|
|
dc.subject.mesh |
Bone Regeneration |
|
|
dc.subject.mesh |
Cell Differentiation |
|
|
dc.subject.mesh |
Osteogenesis |
|
|
dc.subject.mesh |
Chondrogenesis |
|
|
dc.subject.mesh |
Neovascularization, Physiologic |
|
|
dc.subject.mesh |
Mesenchymal Stem Cells |
|
|
dc.subject.mesh |
Bone Regeneration |
|
|
dc.subject.mesh |
Osteogenesis |
|
|
dc.subject.mesh |
Coculture Techniques |
|
|
dc.subject.mesh |
Animals |
|
|
dc.subject.mesh |
Organoids |
|
|
dc.subject.mesh |
Endothelial Cells |
|
|
dc.subject.mesh |
Cell Differentiation |
|
|
dc.subject.mesh |
Humans |
|
|
dc.subject.mesh |
Chondrogenesis |
|
|
dc.subject.mesh |
Mice |
|
|
dc.subject.mesh |
Tissue Engineering |
|
|
dc.subject.mesh |
Neovascularization, Physiologic |
|
|
dc.title |
Synergistic promotion of bone regeneration through co-culture of endothelial cells with mesenchymal stem cells in endochondral ossification organoids. |
|
|
dc.type |
Journal Article |
|
|
utslib.citation.volume |
16 |
|
|
utslib.location.activity |
England |
|
|
utslib.for |
06 Biological Sciences |
|
|
utslib.for |
10 Technology |
|
|
utslib.for |
11 Medical and Health Sciences |
|
|
pubs.organisational-group |
University of Technology Sydney |
|
|
pubs.organisational-group |
University of Technology Sydney/Faculty of Engineering and Information Technology |
|
|
pubs.organisational-group |
University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering |
|
|
pubs.organisational-group |
University of Technology Sydney/UTS Groups |
|
|
pubs.organisational-group |
University of Technology Sydney/UTS Groups/Centre for Health Technologies (CHT) |
|
|
utslib.copyright.status |
open_access |
* |
|
dc.rights.license |
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND 4.0). To view a copy of this license, visit https://creativecommons.org/licenses/by-nc-nd/4.0/ |
|
|
dc.date.updated |
2026-02-03T09:04:56Z |
|
|
pubs.issue |
1 |
|
|
pubs.publication-status |
Published online |
|
|
pubs.volume |
16 |
|
|
utslib.citation.issue |
1 |
|
