Repeated induction of nitric oxide synthase and leishmanicidal activity in murine macrophages.

Cunha, FQ; Assreuy, J; Xu, D; Charles, I; Liew, FY; Moncada, S

Repeated induction of nitric oxide synthase and leishmanicidal activity in murine macrophages.

Cunha, FQ Assreuy, J Xu, D Charles, I Liew, FY Moncada, S

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Publication Type:: Journal Article
Citation:: Eur J Immunol, 1993, 23 (6), pp. 1385 - 1388
Issue Date:: 1993-06

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Field	Value	Language
dc.contributor.author	Cunha, FQ	en_US
dc.contributor.author	Assreuy, J	en_US
dc.contributor.author	Xu, D	en_US
dc.contributor.author	Charles, I	en_US
dc.contributor.author	Liew, FY	en_US
dc.contributor.author	Moncada, S	en_US
dc.date.issued	1993-06	en_US
dc.identifier.citation	Eur J Immunol, 1993, 23 (6), pp. 1385 - 1388	en_US
dc.identifier.issn	0014-2980	en_US
dc.identifier.uri	http://hdl.handle.net/10453/21920
dc.description.abstract	Murine macrophages express high levels of nitric oxide (NO) synthase and produce large amounts of NO when stimulated with interferon-gamma plus lipopolysaccharide in vitro. The expression of NO synthase peaks at 12 h after stimulation and declines rapidly to the background level by 72 h. These macrophages can be repeatedly reactivated to express similar levels of NO synthase. The reactivation is not due to newly divided cells since peritoneal macrophages which do not divide in vitro and J774 cells cultured in the presence of colchicine can also be restimulated to express NO synthase. The reactivation is accompanied by re-expression of NO synthase mRNA, as assessed by polymerase chain reaction analysis. Furthermore, the reactivated macrophages are fully capable of killing the intracellular protozoan parasite Leishmania major.	en_US
dc.language	eng	en_US
dc.relation.ispartof	Eur J Immunol	en_US
dc.relation.isbasedon	10.1002/eji.1830230631	en_US
dc.subject.classification	Immunology	en_US
dc.subject.mesh	Cell Line	en_US
dc.subject.mesh	Macrophages	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Mice	en_US
dc.subject.mesh	Leishmania tropica	en_US
dc.subject.mesh	Leishmaniasis	en_US
dc.subject.mesh	Amino Acid Oxidoreductases	en_US
dc.subject.mesh	RNA, Messenger	en_US
dc.subject.mesh	Oligodeoxyribonucleotides	en_US
dc.subject.mesh	Polymerase Chain Reaction	en_US
dc.subject.mesh	Macrophage Activation	en_US
dc.subject.mesh	Gene Expression	en_US
dc.subject.mesh	Enzyme Induction	en_US
dc.subject.mesh	Base Sequence	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.subject.mesh	Nitric Oxide Synthase	en_US
dc.title	Repeated induction of nitric oxide synthase and leishmanicidal activity in murine macrophages.	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.citation.volume	23	en_US
utslib.location.activity	Germany	en_US
utslib.for	0903 Biomedical Engineering	en_US
utslib.for	1107 Immunology	en_US
dc.location.activity	University of Technology, Sydney
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	6	en_US
pubs.publication-status	Published	en_US
pubs.volume	23	en_US

Abstract:

Murine macrophages express high levels of nitric oxide (NO) synthase and produce large amounts of NO when stimulated with interferon-gamma plus lipopolysaccharide in vitro. The expression of NO synthase peaks at 12 h after stimulation and declines rapidly to the background level by 72 h. These macrophages can be repeatedly reactivated to express similar levels of NO synthase. The reactivation is not due to newly divided cells since peritoneal macrophages which do not divide in vitro and J774 cells cultured in the presence of colchicine can also be restimulated to express NO synthase. The reactivation is accompanied by re-expression of NO synthase mRNA, as assessed by polymerase chain reaction analysis. Furthermore, the reactivated macrophages are fully capable of killing the intracellular protozoan parasite Leishmania major.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/21920