Decreased apoptosis in the ileum and ileal peyer's patches: A feature after infection with rabbit enteropathogenic Escherichia coli O103

Heczko, U; Carthy, CM; O'Brien, BA; Finlay, BB

Decreased apoptosis in the ileum and ileal peyer's patches: A feature after infection with rabbit enteropathogenic Escherichia coli O103

Heczko, U Carthy, CM O'Brien, BA

Finlay, BB

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Publication Type:: Journal Article
Citation:: Infection and Immunity, 2001, 69 (7), pp. 4580 - 4589
Issue Date:: 2001-07-07

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Field	Value	Language
dc.contributor.author	Heczko, U	en_US
dc.contributor.author	Carthy, CM	en_US
dc.contributor.author	O'Brien, BA https://orcid.org/0000-0001-5887-2424	en_US
dc.contributor.author	Finlay, BB	en_US
dc.date.issued	2001-07-07	en_US
dc.identifier.citation	Infection and Immunity, 2001, 69 (7), pp. 4580 - 4589	en_US
dc.identifier.issn	0019-9567	en_US
dc.identifier.uri	http://hdl.handle.net/10453/21957
dc.description.abstract	Significant changes occur in intestinal epithelial cells after infection with enteropathogenic Escherichia coli (EPEC). However, it is unclear whether this pathogen alters rates of apoptosis. By using a naturally occurring weaned rabbit infection model, we determined physiological levels of apoptosis in rabbit ileum and ileal Peyer's patches (PP) and compared them to those found after infection with adherent rabbit EPEC (REPEC O103). Various REPEC O103 strains were first tested in vitro for characteristic virulence features. Rabbits were then inoculated with the REPEC O103 strains that infected cultured cells the most efficiently. After experimental infection, intestinal samples were examined by light and electron microscopy. Simultaneously, ileal apoptosis was assessed by using terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) and caspase 3 assays and by apoptotic cell counts based on morphology (hematoxylin-and-eosin staining). The highest physiological apoptotic indices were measured in PP germinal centers (median = 14.7%), followed by PP domed villi (8.1%), tips of absorptive villi (3.8%), and ileal crypt regions (0.5%). Severe infection with REPEC O103 resulted in a significant decrease in apoptosis in PP germinal centers (determined by TUNEL assay; P = 0.01), in the tips of ileal absorptive villi (determined by H&E staining; P = 0.04), and in whole ileal cell lysates (determined by caspase 3 assay; P = 0.001). We concluded that REPEC O103 does not promote apoptosis. Furthermore, we cannot rule out the possibility that REPEC O103, in fact, decreases apoptotic levels in the rabbit ileum.	en_US
dc.relation.ispartof	Infection and Immunity	en_US
dc.relation.isbasedon	10.1128/IAI.69.7.4580-4589.2001	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Ileum	en_US
dc.subject.mesh	Peyer's Patches	en_US
dc.subject.mesh	Hela Cells	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Rabbits	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Enterobacteriaceae	en_US
dc.subject.mesh	Escherichia coli	en_US
dc.subject.mesh	Escherichia coli Infections	en_US
dc.subject.mesh	Caspases	en_US
dc.subject.mesh	Bacterial Proteins	en_US
dc.subject.mesh	Bacterial Outer Membrane Proteins	en_US
dc.subject.mesh	Escherichia coli Proteins	en_US
dc.subject.mesh	Membrane Proteins	en_US
dc.subject.mesh	Receptors, Cell Surface	en_US
dc.subject.mesh	DNA, Bacterial	en_US
dc.subject.mesh	Shiga Toxin	en_US
dc.subject.mesh	Microscopy, Electron, Scanning	en_US
dc.subject.mesh	Staining and Labeling	en_US
dc.subject.mesh	In Situ Nick-End Labeling	en_US
dc.subject.mesh	Bacterial Adhesion	en_US
dc.subject.mesh	Virulence	en_US
dc.subject.mesh	Apoptosis	en_US
dc.subject.mesh	Plasmids	en_US
dc.subject.mesh	Caspase 3	en_US
dc.subject.mesh	HeLa Cells	en_US
dc.title	Decreased apoptosis in the ileum and ileal peyer's patches: A feature after infection with rabbit enteropathogenic Escherichia coli O103	en_US
dc.type	Journal Article
utslib.citation.volume	7	en_US
utslib.citation.volume	69	en_US
utslib.for	0602 Ecology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access
pubs.issue	7	en_US
pubs.publication-status	Published	en_US
pubs.volume	69	en_US

Abstract:

Significant changes occur in intestinal epithelial cells after infection with enteropathogenic Escherichia coli (EPEC). However, it is unclear whether this pathogen alters rates of apoptosis. By using a naturally occurring weaned rabbit infection model, we determined physiological levels of apoptosis in rabbit ileum and ileal Peyer's patches (PP) and compared them to those found after infection with adherent rabbit EPEC (REPEC O103). Various REPEC O103 strains were first tested in vitro for characteristic virulence features. Rabbits were then inoculated with the REPEC O103 strains that infected cultured cells the most efficiently. After experimental infection, intestinal samples were examined by light and electron microscopy. Simultaneously, ileal apoptosis was assessed by using terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) and caspase 3 assays and by apoptotic cell counts based on morphology (hematoxylin-and-eosin staining). The highest physiological apoptotic indices were measured in PP germinal centers (median = 14.7%), followed by PP domed villi (8.1%), tips of absorptive villi (3.8%), and ileal crypt regions (0.5%). Severe infection with REPEC O103 resulted in a significant decrease in apoptosis in PP germinal centers (determined by TUNEL assay; P = 0.01), in the tips of ileal absorptive villi (determined by H&E staining; P = 0.04), and in whole ileal cell lysates (determined by caspase 3 assay; P = 0.001). We concluded that REPEC O103 does not promote apoptosis. Furthermore, we cannot rule out the possibility that REPEC O103, in fact, decreases apoptotic levels in the rabbit ileum.

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http://hdl.handle.net/10453/21957