Interrelationships between motility, c-AMP, respiration and calcium uptake of ram and boar sperm

Simpson, AM; White, IG

Interrelationships between motility, c-AMP, respiration and calcium uptake of ram and boar sperm

Simpson, AM

White, IG

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Publication Type:: Journal Article
Citation:: Animal Reproduction Science, 1987, 15 (3-4), pp. 189 - 207
Issue Date:: 1987-01-01

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Field	Value	Language
dc.contributor.author	Simpson, AM https://orcid.org/0000-0002-2249-5097	en_US
dc.contributor.author	White, IG	en_US
dc.date.issued	1987-01-01	en_US
dc.identifier.citation	Animal Reproduction Science, 1987, 15 (3-4), pp. 189 - 207	en_US
dc.identifier.issn	0378-4320	en_US
dc.identifier.uri	http://hdl.handle.net/10453/21961
dc.description.abstract	Uptake of radioactive calcium by washed ejaculated ram and epididymal boar spermatozoa was inhibited by theophylline and dibutyryl cyclic AMP (diBc-AMP) which increases cyclic AMP (c-AMP) levels in cells, by the uncouplers 2,4-dinitrophenol (2,4-DNP) and carbonylcyanide m-chlorophenylhydrazone (CCP), by sodium azide, rotenone and antimycin A and by ruthenium red and La3+. Nicotine, eserine and ouabain stimulated the calcium uptake of caput boar sperm and decamethonium inhibited it. Inhibition of calcium uptake was accompanied by a decrease in oxygen uptake in the case of the metabolic inhibitors (rotenone, antimycin A and sodium azide) and substances that interfere with cell calcium transport (ruthenium red and La3+). Respiration was increased in the presence of 2,4-DNP and CCP, and theophylline and diBc-AMP. The phosphodiesterase inhibitors (caffeine and theophylline), diBc-AMP, 2,4-DNP, bicarbonate ion and ouabain increased the motility of caput boar sperm. Most other substances depressed motility. The ionophore A23187 severely inhibited the motility of ram and boar sperm with an accompanying increase in their calcium and oxygen uptake which was largely unaffected by addition of inhibitors, activators and surfactants. This may be explained by the operation of competing mitochondrial and plasma membrane pumps. However, a selective increase in the permeability of the sperm plasma membrane on addition of the polyene antibiotic filipin released calcium accumulated in the presence of A23187. This provides further evidence for the lack of mitochondrial involvement in the influx of calcium produced by the ionophore. © 1987.	en_US
dc.relation.ispartof	Animal Reproduction Science	en_US
dc.relation.isbasedon	10.1016/0378-4320(87)90042-X	en_US
dc.subject.classification	Dairy & Animal Science	en_US
dc.title	Interrelationships between motility, c-AMP, respiration and calcium uptake of ram and boar sperm	en_US
dc.type	Journal Article
utslib.citation.volume	3-4	en_US
utslib.citation.volume	15	en_US
utslib.for	070206 Animal Reproduction	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access
pubs.issue	3-4	en_US
pubs.publication-status	Published	en_US
pubs.volume	15	en_US

Abstract:

Uptake of radioactive calcium by washed ejaculated ram and epididymal boar spermatozoa was inhibited by theophylline and dibutyryl cyclic AMP (diBc-AMP) which increases cyclic AMP (c-AMP) levels in cells, by the uncouplers 2,4-dinitrophenol (2,4-DNP) and carbonylcyanide m-chlorophenylhydrazone (CCP), by sodium azide, rotenone and antimycin A and by ruthenium red and La3+. Nicotine, eserine and ouabain stimulated the calcium uptake of caput boar sperm and decamethonium inhibited it. Inhibition of calcium uptake was accompanied by a decrease in oxygen uptake in the case of the metabolic inhibitors (rotenone, antimycin A and sodium azide) and substances that interfere with cell calcium transport (ruthenium red and La3+). Respiration was increased in the presence of 2,4-DNP and CCP, and theophylline and diBc-AMP. The phosphodiesterase inhibitors (caffeine and theophylline), diBc-AMP, 2,4-DNP, bicarbonate ion and ouabain increased the motility of caput boar sperm. Most other substances depressed motility. The ionophore A23187 severely inhibited the motility of ram and boar sperm with an accompanying increase in their calcium and oxygen uptake which was largely unaffected by addition of inhibitors, activators and surfactants. This may be explained by the operation of competing mitochondrial and plasma membrane pumps. However, a selective increase in the permeability of the sperm plasma membrane on addition of the polyene antibiotic filipin released calcium accumulated in the presence of A23187. This provides further evidence for the lack of mitochondrial involvement in the influx of calcium produced by the ionophore. © 1987.

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