A rapid and sensitive method for the identification of delta-9-tetrahydrocannabinol in oral fluid by liquid chromatography-tandem mass spectrometry

Molnar, A; Lewis, J; Doble, P; Hansen, G; Prolov, T; Fu, S

A rapid and sensitive method for the identification of delta-9-tetrahydrocannabinol in oral fluid by liquid chromatography-tandem mass spectrometry

Molnar, A Lewis, J Doble, P

Hansen, G Prolov, T Fu, S

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Publication Type:: Journal Article
Citation:: Forensic Science International, 2012, 215 (1-3), pp. 92 - 96
Issue Date:: 2012-02-10

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Field	Value	Language
dc.contributor.author	Molnar, A	en_US
dc.contributor.author	Lewis, J	en_US
dc.contributor.author	Doble, P https://orcid.org/0000-0002-8472-1301	en_US
dc.contributor.author	Hansen, G	en_US
dc.contributor.author	Prolov, T	en_US
dc.contributor.author	Fu, S https://orcid.org/0000-0002-6238-3612	en_US
dc.date.available	2011-01-30	en_US
dc.date.issued	2012-02-10	en_US
dc.identifier.citation	Forensic Science International, 2012, 215 (1-3), pp. 92 - 96	en_US
dc.identifier.issn	0379-0738	en_US
dc.identifier.uri	http://hdl.handle.net/10453/22058
dc.description.abstract	A fast and sensitive method was developed for detecting delta-9-tetrahydrocannabinol (THC) in oral fluid by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The method is suitable for samples of small volume and low concentration. For method development and validation, neat oral fluid (200μL) spiked with THC and d 3-THC (internal standard) was extracted via liquid-liquid extraction (LLE). The LLE method had an extraction efficiency of 75% with no significant matrix effects observed in either diluted or neat oral fluid samples. LC was performed on a Zorbax Eclipse XDB-C18 Rapid Resolution HT column (2.1mm×50mm, 1.8μm particle size) with positive electrospray ionisation and selected reaction monitoring. The total run time was an efficient 3.5min in isocratic elution mode. The limit of quantification was 1ng/mL and the analysis was linear over the range of 1-500ng/mL with a correlation coefficient of 0.9998. The imprecision (RSD) of the method was 13% and inaccuracy (MRE) was 4%. The method was subsequently applied to two neat oral fluid samples taken from a chronic cannabis smoker. It was also applied to buffer diluted residual oral fluid samples (n=48) collected using the Cozart RapiScan ® system through the Roadside Drug Testing Program (RDTP) in NSW, Australia. A stability study was performed that revealed freezing or refrigerating resulted in comparable decreases in THC recovery from neat oral fluid at the end of two weeks of storage. Storage at room temperature even for one day invoked significant losses and is not recommended. © 2011.	en_US
dc.relation.ispartof	Forensic Science International	en_US
dc.relation.isbasedon	10.1016/j.forsciint.2011.01.045	en_US
dc.subject.classification	Legal & Forensic Medicine	en_US
dc.subject.mesh	Saliva	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Tetrahydrocannabinol	en_US
dc.subject.mesh	Psychotropic Drugs	en_US
dc.subject.mesh	Specimen Handling	en_US
dc.subject.mesh	Chromatography, Liquid	en_US
dc.subject.mesh	Spectrometry, Mass, Electrospray Ionization	en_US
dc.subject.mesh	Substance Abuse Detection	en_US
dc.subject.mesh	Drug Stability	en_US
dc.subject.mesh	Temperature	en_US
dc.subject.mesh	Automobile Driving	en_US
dc.subject.mesh	Adult	en_US
dc.subject.mesh	Australia	en_US
dc.subject.mesh	Male	en_US
dc.subject.mesh	Tandem Mass Spectrometry	en_US
dc.subject.mesh	Forensic Toxicology	en_US
dc.subject.mesh	Dronabinol	en_US
dc.title	A rapid and sensitive method for the identification of delta-9-tetrahydrocannabinol in oral fluid by liquid chromatography-tandem mass spectrometry	en_US
dc.type	Journal Article
utslib.citation.volume	1-3	en_US
utslib.citation.volume	215	en_US
utslib.for	0399 Other Chemical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CCET - Centre for Clean Energy Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
utslib.copyright.status	closed_access
pubs.issue	1-3	en_US
pubs.publication-status	Published	en_US
pubs.volume	215	en_US

Abstract:

A fast and sensitive method was developed for detecting delta-9-tetrahydrocannabinol (THC) in oral fluid by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The method is suitable for samples of small volume and low concentration. For method development and validation, neat oral fluid (200μL) spiked with THC and d 3-THC (internal standard) was extracted via liquid-liquid extraction (LLE). The LLE method had an extraction efficiency of 75% with no significant matrix effects observed in either diluted or neat oral fluid samples. LC was performed on a Zorbax Eclipse XDB-C18 Rapid Resolution HT column (2.1mm×50mm, 1.8μm particle size) with positive electrospray ionisation and selected reaction monitoring. The total run time was an efficient 3.5min in isocratic elution mode. The limit of quantification was 1ng/mL and the analysis was linear over the range of 1-500ng/mL with a correlation coefficient of 0.9998. The imprecision (RSD) of the method was 13% and inaccuracy (MRE) was 4%. The method was subsequently applied to two neat oral fluid samples taken from a chronic cannabis smoker. It was also applied to buffer diluted residual oral fluid samples (n=48) collected using the Cozart RapiScan ® system through the Roadside Drug Testing Program (RDTP) in NSW, Australia. A stability study was performed that revealed freezing or refrigerating resulted in comparable decreases in THC recovery from neat oral fluid at the end of two weeks of storage. Storage at room temperature even for one day invoked significant losses and is not recommended. © 2011.

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http://hdl.handle.net/10453/22058