Fluorescence in situ hybridization (FISH) detection of nitrite reductase transcripts (nirS mRNA) in Pseudomonas stutzeri biofilms relative to a microscale oxygen gradient

Kofoed, MVW; Nielsen, DA; Revsbech, NP; Schramm, A

Fluorescence in situ hybridization (FISH) detection of nitrite reductase transcripts (nirS mRNA) in Pseudomonas stutzeri biofilms relative to a microscale oxygen gradient

Kofoed, MVW Nielsen, DA

Revsbech, NP

Schramm, A

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Publication Type:: Journal Article
Citation:: Systematic and Applied Microbiology, 2012, 35 (8), pp. 513 - 517
Issue Date:: 2012-12-01

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Field	Value	Language
dc.contributor.author	Kofoed, MVW	en_US
dc.contributor.author	Nielsen, DA https://orcid.org/0000-0001-6678-5937	en_US
dc.contributor.author	Revsbech, NP https://orcid.org/0000-0002-2193-262X	en_US
dc.contributor.author	Schramm, A https://orcid.org/0000-0002-7614-9616	en_US
dc.date.available	2011-12-19	en_US
dc.date.issued	2012-12-01	en_US
dc.identifier.citation	Systematic and Applied Microbiology, 2012, 35 (8), pp. 513 - 517	en_US
dc.identifier.issn	0723-2020	en_US
dc.identifier.uri	http://hdl.handle.net/10453/22150
dc.description.abstract	Microsensor measurements of oxygen were combined with mRNA-targeted fluorescence in situ hybridization (FISH) to relate the expression of nitrite reductase (nirS) to oxygen concentrations in artificial biofilms of the denitrifier Pseudomonas stutzeri. A distinct zone of nirS transcript-containing cells was detected at the oxic-anoxic transition zone, below an oxygen threshold concentration of 0.7-2.5 μM, depending on incubation conditions. Although not a routine technique yet, the possibility of coupling microsensor and mRNA-targeted FISH analyses described here opens for studies addressing microenvironment, identity, and actual activity of microbes in stratified environments at single cell resolution. © 2012 Elsevier GmbH.	en_US
dc.relation.ispartof	Systematic and Applied Microbiology	en_US
dc.relation.isbasedon	10.1016/j.syapm.2011.12.001	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Biofilms	en_US
dc.subject.mesh	Pseudomonas stutzeri	en_US
dc.subject.mesh	Oxygen	en_US
dc.subject.mesh	Nitrite Reductases	en_US
dc.subject.mesh	RNA, Messenger	en_US
dc.subject.mesh	In Situ Hybridization, Fluorescence	en_US
dc.title	Fluorescence in situ hybridization (FISH) detection of nitrite reductase transcripts (nirS mRNA) in Pseudomonas stutzeri biofilms relative to a microscale oxygen gradient	en_US
dc.type	Journal Article
utslib.citation.volume	8	en_US
utslib.citation.volume	35	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0603 Evolutionary Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	8	en_US
pubs.publication-status	Published	en_US
pubs.volume	35	en_US

Abstract:

Microsensor measurements of oxygen were combined with mRNA-targeted fluorescence in situ hybridization (FISH) to relate the expression of nitrite reductase (nirS) to oxygen concentrations in artificial biofilms of the denitrifier Pseudomonas stutzeri. A distinct zone of nirS transcript-containing cells was detected at the oxic-anoxic transition zone, below an oxygen threshold concentration of 0.7-2.5 μM, depending on incubation conditions. Although not a routine technique yet, the possibility of coupling microsensor and mRNA-targeted FISH analyses described here opens for studies addressing microenvironment, identity, and actual activity of microbes in stratified environments at single cell resolution. © 2012 Elsevier GmbH.

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http://hdl.handle.net/10453/22150