Fluorescence in situ hybridization (FISH) detection of nitrite reductase transcripts (nirS mRNA) in Pseudomonas stutzeri biofilms relative to a microscale oxygen gradient

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Journal Article
Systematic and Applied Microbiology, 2012, 35 (8), pp. 513 - 517
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Microsensor measurements of oxygen were combined with mRNA-targeted ?uorescence in situ hybridization (FISH) to relate the expression of nitrite reductase (nirS) to oxygen concentrations in arti?- cial bio?lms of the denitri?er Pseudomonas stutzeri. A distinct zone of nirS transcript-containing cells was detected at the oxicanoxic transition zone, below an oxygen threshold concentration of 0.72.5 M, depending on incubation conditions. Although not a routine technique yet, the possibility of coupling microsensor and mRNA-targeted FISH analyses described here opens for studies addressing microenvironment, identity, and actual activity of microbes in strati?ed environments at single cell resolution
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