Cobalt-induced genotoxicity in male zebrafish (Danio rerio), with implications for reproduction and expression of DNA repair genes

Reinardy, HC; Syrett, JR; Jeffree, RA; Henry, TB; Jha, AN

Cobalt-induced genotoxicity in male zebrafish (Danio rerio), with implications for reproduction and expression of DNA repair genes

Reinardy, HC Syrett, JR Jeffree, RA Henry, TB Jha, AN

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Publication Type:: Journal Article
Citation:: Aquatic Toxicology, 2013, 126 pp. 224 - 230
Issue Date:: 2013-01-05

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Field	Value	Language
dc.contributor.author	Reinardy, HC	en_US
dc.contributor.author	Syrett, JR	en_US
dc.contributor.author	Jeffree, RA	en_US
dc.contributor.author	Henry, TB	en_US
dc.contributor.author	Jha, AN	en_US
dc.date.available	2012-11-07	en_US
dc.date.issued	2013-01-05	en_US
dc.identifier.citation	Aquatic Toxicology, 2013, 126 pp. 224 - 230	en_US
dc.identifier.issn	0166-445X	en_US
dc.identifier.uri	http://hdl.handle.net/10453/22174
dc.description.abstract	Although cobalt (Co) is an environmental contaminant of surface waters in both radioactive (e.g. 60Co) and non-radioactive forms, there is relatively little information about Co toxicity in fishes. The objective of this study was to investigate acute and chronic toxicity of Co in zebrafish, with emphasis on male genotoxicity and implications for reproductive success. The lethal concentration for 50% mortality (LC50) in larval zebrafish exposed (96h) to 0-50mgl-1 Co was 35.3±1.1 (95%C.I.)mgl-1 Co. Adult zebrafish were exposed (13d) to sub-lethal (0-25mgl-1) Co and allowed to spawn every 4d and embryos were collected. After 12-d exposure, fertilisation rate was reduced (6% total eggs fertilised, 25mgl-1) and embryo survival to hatching decreased (60% fertilised eggs survived, 25mgl-1). A concentration-dependent increase in DNA strand breaks was detected in sperm from males exposed (13d) to Co, and DNA damage in sperm returned to control levels after males recovered for 6d in clean water. Induction of DNA repair genes (rad51, xrcc5, and xrcc6) in testes was complex and not directly related to Co concentration, although there was significant induction in fish exposed to 15 and 25mgl-1 Co relative to controls. Induction of 4.0±0.9, 2.5±0.7, and 3.1±0.7-fold change (mean±S.E.M. for rad51, xrcc5, and xrcc6, respectively) was observed in testes at the highest Co concentration (25mgl-1). Expression of these genes was not altered in offspring (larvae) spawned after 12-d exposure. Chronic exposure to Co resulted in DNA damage in sperm, induction of DNA repair genes in testes, and indications of reduced reproductive success. © 2012 Elsevier B.V.	en_US
dc.relation.ispartof	Aquatic Toxicology	en_US
dc.relation.isbasedon	10.1016/j.aquatox.2012.11.007	en_US
dc.subject.classification	Toxicology	en_US
dc.subject.mesh	Testis	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Zebrafish	en_US
dc.subject.mesh	DNA Damage	en_US
dc.subject.mesh	Cobalt	en_US
dc.subject.mesh	Water Pollutants, Chemical	en_US
dc.subject.mesh	Lethal Dose 50	en_US
dc.subject.mesh	DNA Repair	en_US
dc.subject.mesh	Gene Expression Regulation	en_US
dc.subject.mesh	Reproduction	en_US
dc.subject.mesh	Time Factors	en_US
dc.subject.mesh	Male	en_US
dc.title	Cobalt-induced genotoxicity in male zebrafish (Danio rerio), with implications for reproduction and expression of DNA repair genes	en_US
dc.type	Journal Article
utslib.citation.volume	126	en_US
utslib.for	0602 Ecology	en_US
utslib.for	03 Chemical Sciences	en_US
utslib.for	05 Environmental Sciences	en_US
utslib.for	06 Biological Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	126	en_US

Abstract:

Although cobalt (Co) is an environmental contaminant of surface waters in both radioactive (e.g. 60Co) and non-radioactive forms, there is relatively little information about Co toxicity in fishes. The objective of this study was to investigate acute and chronic toxicity of Co in zebrafish, with emphasis on male genotoxicity and implications for reproductive success. The lethal concentration for 50% mortality (LC50) in larval zebrafish exposed (96h) to 0-50mgl-1 Co was 35.3±1.1 (95%C.I.)mgl-1 Co. Adult zebrafish were exposed (13d) to sub-lethal (0-25mgl-1) Co and allowed to spawn every 4d and embryos were collected. After 12-d exposure, fertilisation rate was reduced (6% total eggs fertilised, 25mgl-1) and embryo survival to hatching decreased (60% fertilised eggs survived, 25mgl-1). A concentration-dependent increase in DNA strand breaks was detected in sperm from males exposed (13d) to Co, and DNA damage in sperm returned to control levels after males recovered for 6d in clean water. Induction of DNA repair genes (rad51, xrcc5, and xrcc6) in testes was complex and not directly related to Co concentration, although there was significant induction in fish exposed to 15 and 25mgl-1 Co relative to controls. Induction of 4.0±0.9, 2.5±0.7, and 3.1±0.7-fold change (mean±S.E.M. for rad51, xrcc5, and xrcc6, respectively) was observed in testes at the highest Co concentration (25mgl-1). Expression of these genes was not altered in offspring (larvae) spawned after 12-d exposure. Chronic exposure to Co resulted in DNA damage in sperm, induction of DNA repair genes in testes, and indications of reduced reproductive success. © 2012 Elsevier B.V.

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http://hdl.handle.net/10453/22174