Androgens rapidly activate nuclear factor-kappa B via intracellular Ca2+ signalling in human vascular endothelial cells

McGrath, KC; Li, XH; Gaus, K; Williams, P; Celermajer, DS; Handelsman, DJ; Heather, AK

Androgens rapidly activate nuclear factor-kappa B via intracellular Ca2+ signalling in human vascular endothelial cells

McGrath, KC

Li, XH Gaus, K Williams, P Celermajer, DS Handelsman, DJ Heather, AK

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Publisher:: OMICS Group
Publication Type:: Journal Article
Citation:: Journal of Steroids & Hormonal Science, 2012, S2 pp. 1 - 6
Issue Date:: 2012-01

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Field	Value	Language
dc.contributor.author	McGrath, KC https://orcid.org/0000-0002-6244-3929	en_US
dc.contributor.author	Li, XH	en_US
dc.contributor.author	Gaus, K	en_US
dc.contributor.author	Williams, P	en_US
dc.contributor.author	Celermajer, DS	en_US
dc.contributor.author	Handelsman, DJ	en_US
dc.contributor.author	Heather, AK	en_US
dc.date.issued	2012-01	en_US
dc.identifier.citation	Journal of Steroids & Hormonal Science, 2012, S2 pp. 1 - 6	en_US
dc.identifier.issn	2157-7536	en_US
dc.identifier.uri	http://hdl.handle.net/10453/22182
dc.description.abstract	There exists a striking gender difference in the incidence of atherosclerosis. Androgen exposure may predispose men to earlier onset atherosclerosis. We previously demonstrated that the potent androgen, dihydrotestosterone, enhanced the binding of monocytes to endothelial cells, via androgen receptor/nuclear factor kappa B-dependent expression of the cell adhesion molecules, vascular cell adhesion molecule-1 and intercellular cell adhesion molecule-1. We now show that testosterone and dihydrotestosterone can also induce a novel, non-genomic pathway that leads to the rapid activation of nuclear factor-kappa B via intracellular Ca2+ signalling, initiated at the plasma membrane. Human umbilical vein endothelial cells exposed to 6-60 nM testosterone or dihydrotestosterone showed a rapid increase in intracellular calcium levels. The testosterone or dihydrotestosterone effect on increased intracellular calcium could not be abrogated by pre-incubation with androgen receptor antagonist, hydroxyflutamide, or by U73122, an inhibitor of intracellular calcium release from endoplasmic reticulum stores.	en_US
dc.publisher	OMICS Group	en_US
dc.relation.ispartof	Journal of Steroids & Hormonal Science	en_US
dc.title	Androgens rapidly activate nuclear factor-kappa B via intracellular Ca2+ signalling in human vascular endothelial cells	en_US
dc.type	Journal Article
utslib.citation.volume	S2	en_US
utslib.for	0605 Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Medical and Molecular Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access
pubs.consider-herdc	true	en_US
pubs.volume	S2	en_US

Abstract:

There exists a striking gender difference in the incidence of atherosclerosis. Androgen exposure may predispose men to earlier onset atherosclerosis. We previously demonstrated that the potent androgen, dihydrotestosterone, enhanced the binding of monocytes to endothelial cells, via androgen receptor/nuclear factor kappa B-dependent expression of the cell adhesion molecules, vascular cell adhesion molecule-1 and intercellular cell adhesion molecule-1. We now show that testosterone and dihydrotestosterone can also induce a novel, non-genomic pathway that leads to the rapid activation of nuclear factor-kappa B via intracellular Ca2+ signalling, initiated at the plasma membrane. Human umbilical vein endothelial cells exposed to 6-60 nM testosterone or dihydrotestosterone showed a rapid increase in intracellular calcium levels. The testosterone or dihydrotestosterone effect on increased intracellular calcium could not be abrogated by pre-incubation with androgen receptor antagonist, hydroxyflutamide, or by U73122, an inhibitor of intracellular calcium release from endoplasmic reticulum stores.

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http://hdl.handle.net/10453/22182