Performance of single and multi-strain probiotics during hatchery production of Greenshell <sup>™</sup> mussel larvae, Perna canaliculus

Kesarcodi-Watson, A; Kaspar, H; Lategan, MJ; Gibson, L

Performance of single and multi-strain probiotics during hatchery production of Greenshell <sup>™</sup> mussel larvae, Perna canaliculus

Kesarcodi-Watson, A Kaspar, H Lategan, MJ Gibson, L

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Publication Type:: Journal Article
Citation:: Aquaculture, 2012, 354-355 pp. 56 - 63
Issue Date:: 2012-07-02

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Field	Value	Language
dc.contributor.author	Kesarcodi-Watson, A	en_US
dc.contributor.author	Kaspar, H	en_US
dc.contributor.author	Lategan, MJ	en_US
dc.contributor.author	Gibson, L	en_US
dc.date.issued	2012-07-02	en_US
dc.identifier.citation	Aquaculture, 2012, 354-355 pp. 56 - 63	en_US
dc.identifier.issn	0044-8486	en_US
dc.identifier.uri	http://hdl.handle.net/10453/22191
dc.description.abstract	Earlier work identified two novel strains of probiotic bacteria, Alteromonas macleodii 0444 and Neptunomonas sp. 0536, for Greenshell ™ mussel (Perna canaliculus) larvae. Herein, we investigated whether the combination of the two probiotics in a multi-strain mix provided a) enhanced larval production during routine rearing and b) improved larval protection during two separate pathogen-challenge tests (Vibrio sp. DO1 and Vibrio splendidus). The response of larvae to multi-strain or single-strain probiotic administration was compared to that of larvae without probiotics. Two concentrations of each probiotic were tested (10 7 and 10 8CFUml -1). Addition of 10 8CFUml -1 multi-strain mix in the routine rearing of larvae yielded smaller-sized larvae and a lower feeding rate when compared with the 10 7CFUml -1 and control groups. During the challenge test against Vibrio sp. DO1 and V. splendidus, protection by single-strain probiotic administration was observed at both levels of probiotic administration (10 7 and 10 8CFUml -1), with no apparent added protection from multi-strain probiotics. Although 10 8CFUml -1 levels provided protection against pathogen attack, they were also potentially detrimental to normal larval rearing when administered in combination and, as such, where administration of A. macleodii 0444 and Neptunomonas sp. 0536 would be applied together, a multi-strain mix of probiotics at 10 7CFUml -1, is recommended as the best concentration of each probiotic. © 2012 Elsevier B.V.	en_US
dc.relation.ispartof	Aquaculture	en_US
dc.relation.isbasedon	10.1016/j.aquaculture.2012.04.026	en_US
dc.subject.classification	Fisheries	en_US
dc.title	Performance of single and multi-strain probiotics during hatchery production of Greenshell <sup>™</sup> mussel larvae, Perna canaliculus	en_US
dc.type	Journal Article
utslib.citation.volume	354-355	en_US
utslib.for	0704 Fisheries Sciences	en_US
utslib.for	0608 Zoology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	354-355	en_US

Abstract:

Earlier work identified two novel strains of probiotic bacteria, Alteromonas macleodii 0444 and Neptunomonas sp. 0536, for Greenshell ™ mussel (Perna canaliculus) larvae. Herein, we investigated whether the combination of the two probiotics in a multi-strain mix provided a) enhanced larval production during routine rearing and b) improved larval protection during two separate pathogen-challenge tests (Vibrio sp. DO1 and Vibrio splendidus). The response of larvae to multi-strain or single-strain probiotic administration was compared to that of larvae without probiotics. Two concentrations of each probiotic were tested (10 7 and 10 8CFUml -1). Addition of 10 8CFUml -1 multi-strain mix in the routine rearing of larvae yielded smaller-sized larvae and a lower feeding rate when compared with the 10 7CFUml -1 and control groups. During the challenge test against Vibrio sp. DO1 and V. splendidus, protection by single-strain probiotic administration was observed at both levels of probiotic administration (10 7 and 10 8CFUml -1), with no apparent added protection from multi-strain probiotics. Although 10 8CFUml -1 levels provided protection against pathogen attack, they were also potentially detrimental to normal larval rearing when administered in combination and, as such, where administration of A. macleodii 0444 and Neptunomonas sp. 0536 would be applied together, a multi-strain mix of probiotics at 10 7CFUml -1, is recommended as the best concentration of each probiotic. © 2012 Elsevier B.V.

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http://hdl.handle.net/10453/22191