Biomarkers of exposure and effect in a lacertid lizard (Podarcis bocagei Seoane) exposed to chlorpyrifos

Amaral, MJ; Sanchez-Hernandez, JC; Bicho, RC; Carretero, MA; Valente, R; Faustino, AMR; Soares, AMVM; Mann, RM

Biomarkers of exposure and effect in a lacertid lizard (Podarcis bocagei Seoane) exposed to chlorpyrifos

Amaral, MJ Sanchez-Hernandez, JC Bicho, RC Carretero, MA Valente, R Faustino, AMR Soares, AMVM Mann, RM

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Publication Type:: Journal Article
Citation:: Environmental Toxicology and Chemistry, 2012, 31 (10), pp. 2345 - 2353
Issue Date:: 2012-10-01

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Field	Value	Language
dc.contributor.author	Amaral, MJ	en_US
dc.contributor.author	Sanchez-Hernandez, JC	en_US
dc.contributor.author	Bicho, RC	en_US
dc.contributor.author	Carretero, MA	en_US
dc.contributor.author	Valente, R	en_US
dc.contributor.author	Faustino, AMR	en_US
dc.contributor.author	Soares, AMVM	en_US
dc.contributor.author	Mann, RM https://orcid.org/0000-0001-7696-0751	en_US
dc.date.available	2012-06-13	en_US
dc.date.issued	2012-10-01	en_US
dc.identifier.citation	Environmental Toxicology and Chemistry, 2012, 31 (10), pp. 2345 - 2353	en_US
dc.identifier.issn	0730-7268	en_US
dc.identifier.uri	http://hdl.handle.net/10453/22788
dc.description.abstract	In Europe, reptiles have been recently included in environmental risk-assessment processes for registration of plant-protection products. However, data on toxicity effects of most compounds are lacking. Chlorpyrifos is the most commonly used organophosphorus insecticide worldwide. In the present study, the authors exposed a lacertid lizard, Podarcis bocagei, to sublethal concentrations of chlorpyrifos. Individuals were exposed through spiked food for a period of 20 d (low dose 0.12mg/kg/d, high dose 1.57mg/kg/d). After exposure, various biomarkers of exposure and effect were evaluated, including the activities of glutathione S-transferase and enzymes involved in the glutathione redox cycle, glutathione concentrations, activities of esterases, liver and testes histopathologies, as well as locomotory and predatory behavior. The results indicate that sublethal, subchronic exposure to chlorpyrifos can affect P. bocagei in a dose-dependent manner. Adverse effects occurred at both the subindividual and individual levels, including inhibition of carboxylesterases and cholinesterases (ChEs), liver histopathological changes, and altered predatory behaviors. Animals exposed to chlorpyrifos took more time to capture and subdue prey items. The results suggest a link between effects at subindividual levels of organization with those observed at the whole individual level after exposure to environmentally realistic dosages of chlorpyrifos. © 2012 SETAC.	en_US
dc.relation.ispartof	Environmental Toxicology and Chemistry	en_US
dc.relation.isbasedon	10.1002/etc.1955	en_US
dc.subject.classification	Environmental Sciences	en_US
dc.subject.mesh	Liver	en_US
dc.subject.mesh	Testis	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Lizards	en_US
dc.subject.mesh	Chlorpyrifos	en_US
dc.subject.mesh	Carboxylic Ester Hydrolases	en_US
dc.subject.mesh	Cholinesterases	en_US
dc.subject.mesh	Glutathione Transferase	en_US
dc.subject.mesh	Glutathione	en_US
dc.subject.mesh	Biomarkers	en_US
dc.subject.mesh	Insecticides	en_US
dc.subject.mesh	Male	en_US
dc.subject.mesh	Motor Activity	en_US
dc.subject.mesh	Predatory Behavior	en_US
dc.subject.mesh	Toxicity Tests, Subacute	en_US
dc.title	Biomarkers of exposure and effect in a lacertid lizard (Podarcis bocagei Seoane) exposed to chlorpyrifos	en_US
dc.type	Journal Article
utslib.citation.volume	10	en_US
utslib.citation.volume	31	en_US
utslib.for	0399 Other Chemical Sciences	en_US
utslib.for	03 Chemical Sciences	en_US
utslib.for	05 Environmental Sciences	en_US
utslib.for	06 Biological Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	10	en_US
pubs.publication-status	Published	en_US
pubs.volume	31	en_US

Abstract:

In Europe, reptiles have been recently included in environmental risk-assessment processes for registration of plant-protection products. However, data on toxicity effects of most compounds are lacking. Chlorpyrifos is the most commonly used organophosphorus insecticide worldwide. In the present study, the authors exposed a lacertid lizard, Podarcis bocagei, to sublethal concentrations of chlorpyrifos. Individuals were exposed through spiked food for a period of 20 d (low dose 0.12mg/kg/d, high dose 1.57mg/kg/d). After exposure, various biomarkers of exposure and effect were evaluated, including the activities of glutathione S-transferase and enzymes involved in the glutathione redox cycle, glutathione concentrations, activities of esterases, liver and testes histopathologies, as well as locomotory and predatory behavior. The results indicate that sublethal, subchronic exposure to chlorpyrifos can affect P. bocagei in a dose-dependent manner. Adverse effects occurred at both the subindividual and individual levels, including inhibition of carboxylesterases and cholinesterases (ChEs), liver histopathological changes, and altered predatory behaviors. Animals exposed to chlorpyrifos took more time to capture and subdue prey items. The results suggest a link between effects at subindividual levels of organization with those observed at the whole individual level after exposure to environmentally realistic dosages of chlorpyrifos. © 2012 SETAC.

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http://hdl.handle.net/10453/22788