Effect of Multipurpose Solutions on Cell Morphology and Cytokine Production by Corneal Epithelial Cells

Publisher:
American Academy of Optometry
Publication Type:
Journal Article
Citation:
Optometry And Vision Science, 2012, 89 (10), pp. 1460 - 1467
Issue Date:
2012-01
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Purpose. We sought to determine whether human corneal limbal epithelial cells (HCLE) differ in their physiological response and production of inflammatory mediators during exposure to two multipurpose disinfecting solutions that differ in only four excipients. Methods. HCLE were exposed to dilutions (1%20%) of OPTI-FREE Express and OPTI-FREE RepleniSH for 2, 6, or 18 h. Cell numbers were measured using CyQuant and metabolic activity assays. Morphology, viability, and apoptotic changes were examined by confocal microscopy after staining with Calcein AM/propidium iodide or Hoechst 33,342/propidiun iodide/YO-PRO-1. Cytokine responses and arachidonic acid metabolites were examined by enzyme-linked immunosorbent assay.
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