Effect of hydrogen peroxide oxidation systems on human urinary steroid profiles

Kuzhiumparambil, U; Fu, S

Effect of hydrogen peroxide oxidation systems on human urinary steroid profiles

Kuzhiumparambil, U

Fu, S

Permalink

Publication Type:: Journal Article
Citation:: Analytical Methods, 2013, 5 (17), pp. 4402 - 4408
Issue Date:: 2013-09-17

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Adobe PDF

Download full textAdobe PDF (688.36 kB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Kuzhiumparambil, U https://orcid.org/0000-0003-0582-6779	en_US
dc.contributor.author	Fu, S https://orcid.org/0000-0002-6238-3612	en_US
dc.date.issued	2013-09-17	en_US
dc.identifier.citation	Analytical Methods, 2013, 5 (17), pp. 4402 - 4408	en_US
dc.identifier.issn	1759-9660	en_US
dc.identifier.uri	http://hdl.handle.net/10453/23563
dc.description.abstract	In sports drug testing the steroid profile is the most versatile and informative screening tool for the detection of steroid abuse. Despite the introduction of observed urine collection procedures by the World Anti-Doping Agency (WADA), chemical manipulation of urine specimens by athletes to conceal drug use still occurs and poses an ongoing challenge for doping control laboratories worldwide. In vitro urine adulteration using highly oxidative chemicals have been reported several times in the past. In this study we report the effect of two oxidising agents, Fenton's reagent and peroxidase-peroxide system on the human urinary steroid profile. Varying concentrations of these oxidants were reacted with urine and the reactions monitored by gas chromatography-mass spectrometry. A significant decrease in the absolute concentrations of androsterone, etiocholanolone, 5α-androstane-3α, 17β-diol, 5β-androstane-3α,17β-diol and epitestosterone was observed with consequent alteration of the steroid profile ratios. Adulteration of urine sample with these oxidants can thus mask the abnormality in a steroidal profile following steroid abuse. Drug testing authorities should take into account the effects of these oxidizing adulterants while interpreting the steroid profile data for doping control purposes. © The Royal Society of Chemistry.	en_US
dc.relation.ispartof	Analytical Methods	en_US
dc.relation.isbasedon	10.1039/c3ay40181b	en_US
dc.title	Effect of hydrogen peroxide oxidation systems on human urinary steroid profiles	en_US
dc.type	Journal Article
utslib.citation.volume	17	en_US
utslib.citation.volume	5	en_US
utslib.for	0301 Analytical Chemistry	en_US
utslib.for	0399 Other Chemical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
pubs.organisational-group	/University of Technology Sydney/Strength - CCET - Centre for Clean Energy Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
utslib.copyright.status	open_access
pubs.issue	17	en_US
pubs.publication-status	Published	en_US
pubs.volume	5	en_US

Abstract:

In sports drug testing the steroid profile is the most versatile and informative screening tool for the detection of steroid abuse. Despite the introduction of observed urine collection procedures by the World Anti-Doping Agency (WADA), chemical manipulation of urine specimens by athletes to conceal drug use still occurs and poses an ongoing challenge for doping control laboratories worldwide. In vitro urine adulteration using highly oxidative chemicals have been reported several times in the past. In this study we report the effect of two oxidising agents, Fenton's reagent and peroxidase-peroxide system on the human urinary steroid profile. Varying concentrations of these oxidants were reacted with urine and the reactions monitored by gas chromatography-mass spectrometry. A significant decrease in the absolute concentrations of androsterone, etiocholanolone, 5α-androstane-3α, 17β-diol, 5β-androstane-3α,17β-diol and epitestosterone was observed with consequent alteration of the steroid profile ratios. Adulteration of urine sample with these oxidants can thus mask the abnormality in a steroidal profile following steroid abuse. Drug testing authorities should take into account the effects of these oxidizing adulterants while interpreting the steroid profile data for doping control purposes. © The Royal Society of Chemistry.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/23563