The type II secretion system and its ubiquitous lipoprotein substrate, SslE, are required for biofilm formation and virulence of enteropathogenic Escherichia coli

Baldi, DL; Higginson, EE; Hocking, DM; Praszkier, J; Cavaliere, R; James, CE; Bennett-Wood, V; Azzopardi, KI; Turnbull, L; Lithgow, T; Robins-Browne, RM; Whitchurch, CB; Tauschek, M

The type II secretion system and its ubiquitous lipoprotein substrate, SslE, are required for biofilm formation and virulence of enteropathogenic Escherichia coli

Baldi, DL Higginson, EE Hocking, DM Praszkier, J Cavaliere, R

James, CE Bennett-Wood, V Azzopardi, KI Turnbull, L

Lithgow, T Robins-Browne, RM Whitchurch, CB

Tauschek, M

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Publication Type:: Journal Article
Citation:: Infection and Immunity, 2012, 80 (6), pp. 2042 - 2052
Issue Date:: 2012-01-01

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Field	Value	Language
dc.contributor.author	Baldi, DL	en_US
dc.contributor.author	Higginson, EE	en_US
dc.contributor.author	Hocking, DM	en_US
dc.contributor.author	Praszkier, J	en_US
dc.contributor.author	Cavaliere, R https://orcid.org/0000-0002-4709-5081	en_US
dc.contributor.author	James, CE	en_US
dc.contributor.author	Bennett-Wood, V	en_US
dc.contributor.author	Azzopardi, KI	en_US
dc.contributor.author	Turnbull, L https://orcid.org/0000-0002-9255-9033	en_US
dc.contributor.author	Lithgow, T	en_US
dc.contributor.author	Robins-Browne, RM	en_US
dc.contributor.author	Whitchurch, CB https://orcid.org/0000-0003-2296-3791	en_US
dc.contributor.author	Tauschek, M	en_US
dc.date.issued	2012-01-01	en_US
dc.identifier.citation	Infection and Immunity, 2012, 80 (6), pp. 2042 - 2052	en_US
dc.identifier.issn	0019-9567	en_US
dc.identifier.uri	http://hdl.handle.net/10453/26008
dc.description.abstract	Enteropathogenic Escherichia coli (EPEC) is a major cause of diarrhea in infants in developing countries. We have identified a functional type II secretion system (T2SS) in EPEC that is homologous to the pathway responsible for the secretion of heat-labile enterotoxin by enterotoxigenic E. coli. The wild-type EPEC T2SS was able to secrete a heat-labile enterotoxin reporter, but an isogenic T2SS mutant could not. We showed that the major substrate of the T2SS in EPEC is SslE, an outer membrane lipoprotein (formerly known as YghJ), and that a functional T2SS is essential for biofilm formation by EPEC. T2SS and SslE mutants were arrested at the microcolony stage of biofilm formation, suggesting that the T2SS is involved in the development of mature biofilms and that SslE is a dominant effector of biofilm development. Moreover, the T2SS was required for virulence, as infection of rabbits with a rabbit-specific EPEC strain carrying a mutation in either the T2SS or SslE resulted in significantly reduced intestinal colonization and milder disease. © 2012, American Society for Microbiology.	en_US
dc.relation	http://purl.org/au-research/grants/nhmrc/GNT0571905
dc.relation.ispartof	Infection and Immunity	en_US
dc.relation.isbasedon	10.1128/IAI.06160-11	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Cell Membrane	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Rabbits	en_US
dc.subject.mesh	Biofilms	en_US
dc.subject.mesh	Escherichia coli Proteins	en_US
dc.subject.mesh	Virulence Factors	en_US
dc.subject.mesh	Virulence	en_US
dc.subject.mesh	Gene Expression Regulation, Bacterial	en_US
dc.subject.mesh	Substrate Specificity	en_US
dc.subject.mesh	Mutation	en_US
dc.subject.mesh	Enteropathogenic Escherichia coli	en_US
dc.title	The type II secretion system and its ubiquitous lipoprotein substrate, SslE, are required for biofilm formation and virulence of enteropathogenic Escherichia coli	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.citation.volume	80	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	6	en_US
pubs.publication-status	Published	en_US
pubs.volume	80	en_US

Abstract:

Enteropathogenic Escherichia coli (EPEC) is a major cause of diarrhea in infants in developing countries. We have identified a functional type II secretion system (T2SS) in EPEC that is homologous to the pathway responsible for the secretion of heat-labile enterotoxin by enterotoxigenic E. coli. The wild-type EPEC T2SS was able to secrete a heat-labile enterotoxin reporter, but an isogenic T2SS mutant could not. We showed that the major substrate of the T2SS in EPEC is SslE, an outer membrane lipoprotein (formerly known as YghJ), and that a functional T2SS is essential for biofilm formation by EPEC. T2SS and SslE mutants were arrested at the microcolony stage of biofilm formation, suggesting that the T2SS is involved in the development of mature biofilms and that SslE is a dominant effector of biofilm development. Moreover, the T2SS was required for virulence, as infection of rabbits with a rabbit-specific EPEC strain carrying a mutation in either the T2SS or SslE resulted in significantly reduced intestinal colonization and milder disease. © 2012, American Society for Microbiology.

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http://hdl.handle.net/10453/26008