Type 3 secretion system effector genotype and secretion phenotype of longitudinally collected Pseudomonas aeruginosa isolates from young children diagnosed with cystic fibrosis following newborn screening

Hu, H; Harmer, C; Anuj, S; Wainwright, CE; Manos, J; Cheney, J; Harbour, C; Zablotska, I; Turnbull, L; Whitchurch, CB; Grimwood, K; Rose, B

Type 3 secretion system effector genotype and secretion phenotype of longitudinally collected Pseudomonas aeruginosa isolates from young children diagnosed with cystic fibrosis following newborn screening

Hu, H Harmer, C Anuj, S Wainwright, CE Manos, J Cheney, J Harbour, C Zablotska, I Turnbull, L

Whitchurch, CB

Grimwood, K Rose, B

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Publication Type:: Journal Article
Citation:: Clinical Microbiology and Infection, 2013, 19 (3), pp. 266 - 272
Issue Date:: 2013-01-01

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Field	Value	Language
dc.contributor.author	Hu, H	en_US
dc.contributor.author	Harmer, C	en_US
dc.contributor.author	Anuj, S	en_US
dc.contributor.author	Wainwright, CE	en_US
dc.contributor.author	Manos, J	en_US
dc.contributor.author	Cheney, J	en_US
dc.contributor.author	Harbour, C	en_US
dc.contributor.author	Zablotska, I	en_US
dc.contributor.author	Turnbull, L https://orcid.org/0000-0002-9255-9033	en_US
dc.contributor.author	Whitchurch, CB https://orcid.org/0000-0003-2296-3791	en_US
dc.contributor.author	Grimwood, K	en_US
dc.contributor.author	Rose, B	en_US
dc.date.issued	2013-01-01	en_US
dc.identifier.citation	Clinical Microbiology and Infection, 2013, 19 (3), pp. 266 - 272	en_US
dc.identifier.issn	1198-743X	en_US
dc.identifier.uri	http://hdl.handle.net/10453/26357
dc.description.abstract	Studies of the type 3 secretion system (T3SS) in Pseudomonas aeruginosa isolates from chronically infected older children and adults with cystic fibrosis (CF) show a predominantly exoS+/exoU- (exoS+) genotype and loss of T3SS effector secretion over time. Relatively little is known about the role of the T3SS in the pathogenesis of early P. aeruginosa infection in the CF airway. In this longitudinal study, 168 P. aeruginosa isolates from 58 children diagnosed with CF following newborn screening and 47 isolates from homes of families with or without children with CF were genotyped by pulsed-field gel electrophoresis (PFGE) and T3SS genotype and phenotype determined using multiplex PCR and western blotting. Associations were sought between T3SS data and clinical variables and comparisons made between T3SS data of clinical and environmental PFGE genotypes. Seventy-seven of the 92 clinical strains were exoS+ (71% secretors (ExoS+)) and 15 were exoU+ (93% secretors (ExoU+)). Initial exoS+ strains were five times more likely to secrete ExoS than subsequent exoS+ strains at first isolation. The proportion of ExoS+ strains declined with increasing age at acquisition. No associations were found between T3SS characteristics and gender, site of isolation, exacerbation, a persistent strain or pulmonary outcomes. Fourteen of the 23 environmental strains were exoS+ (79% ExoS+) and nine were exoU+ (33% ExoU+). The exoU+ environmental strains were significantly less likely to secrete ExoU than clinical strains. This study provides new insight into the T3SS characteristics of P. aeruginosa isolated from the CF airway early in life. © 2012 The Authors. Clinical Microbiology and Infection © 2012 European Society of Clinical Microbiology and Infectious Diseases.	en_US
dc.relation.ispartof	Clinical Microbiology and Infection	en_US
dc.relation.isbasedon	10.1111/j.1469-0691.2012.03770.x	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Pseudomonas aeruginosa	en_US
dc.subject.mesh	Pseudomonas Infections	en_US
dc.subject.mesh	Cystic Fibrosis	en_US
dc.subject.mesh	Bronchopneumonia	en_US
dc.subject.mesh	ADP Ribose Transferases	en_US
dc.subject.mesh	Bacterial Proteins	en_US
dc.subject.mesh	Bacterial Toxins	en_US
dc.subject.mesh	Virulence Factors	en_US
dc.subject.mesh	Blotting, Western	en_US
dc.subject.mesh	Electrophoresis, Gel, Pulsed-Field	en_US
dc.subject.mesh	Longitudinal Studies	en_US
dc.subject.mesh	Genotype	en_US
dc.subject.mesh	Phenotype	en_US
dc.subject.mesh	Child	en_US
dc.subject.mesh	Child, Preschool	en_US
dc.subject.mesh	Infant	en_US
dc.subject.mesh	Infant, Newborn	en_US
dc.subject.mesh	Female	en_US
dc.subject.mesh	Male	en_US
dc.subject.mesh	Molecular Typing	en_US
dc.subject.mesh	Bacterial Secretion Systems	en_US
dc.subject.mesh	Multiplex Polymerase Chain Reaction	en_US
dc.title	Type 3 secretion system effector genotype and secretion phenotype of longitudinally collected Pseudomonas aeruginosa isolates from young children diagnosed with cystic fibrosis following newborn screening	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	19	en_US
utslib.for	1103 Clinical Sciences	en_US
utslib.for	1117 Public Health and Health Services	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	19	en_US

Abstract:

Studies of the type 3 secretion system (T3SS) in Pseudomonas aeruginosa isolates from chronically infected older children and adults with cystic fibrosis (CF) show a predominantly exoS+/exoU- (exoS+) genotype and loss of T3SS effector secretion over time. Relatively little is known about the role of the T3SS in the pathogenesis of early P. aeruginosa infection in the CF airway. In this longitudinal study, 168 P. aeruginosa isolates from 58 children diagnosed with CF following newborn screening and 47 isolates from homes of families with or without children with CF were genotyped by pulsed-field gel electrophoresis (PFGE) and T3SS genotype and phenotype determined using multiplex PCR and western blotting. Associations were sought between T3SS data and clinical variables and comparisons made between T3SS data of clinical and environmental PFGE genotypes. Seventy-seven of the 92 clinical strains were exoS+ (71% secretors (ExoS+)) and 15 were exoU+ (93% secretors (ExoU+)). Initial exoS+ strains were five times more likely to secrete ExoS than subsequent exoS+ strains at first isolation. The proportion of ExoS+ strains declined with increasing age at acquisition. No associations were found between T3SS characteristics and gender, site of isolation, exacerbation, a persistent strain or pulmonary outcomes. Fourteen of the 23 environmental strains were exoS+ (79% ExoS+) and nine were exoU+ (33% ExoU+). The exoU+ environmental strains were significantly less likely to secrete ExoU than clinical strains. This study provides new insight into the T3SS characteristics of P. aeruginosa isolated from the CF airway early in life. © 2012 The Authors. Clinical Microbiology and Infection © 2012 European Society of Clinical Microbiology and Infectious Diseases.

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http://hdl.handle.net/10453/26357