A portable explosive detector based on fluorescence quenching of pyrene deposited on coloured wax-printed µpADs

RSC Publishing
Publication Type:
Journal Article
Lab on a Chip - Miniaturisation for Chemistry,Physics, Biology, materials science and bioengineering, 2013, 13 (21), pp. 4164 - 4172
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A new technique for the detection of explosives has been developed based on fluorescence quenching of pyrene on paper-based analytical devices (µPADs). Wax barriers were generated (150 °C, 5 min) using ten different colours. Magenta was found as the most suitable wax colour for the generation of the hydrophobic barriers with a nominal width of 120 µm resulting in fully functioning hydrophobic barriers. One microliter of 0.5 mg mL-1 pyrene dissolved in an 80:20 methanolwater solution was deposited on the hydrophobic circle (5 mm diameter) to produce the active microchip device. Under ultra-violet (UV) illumination, ten different organic explosives were detected using the µPAD, with limits of detection ranging from 100600 ppm. A prototype of a portable battery operated instrument using a 3 W power UV light-emitting-diode (LED) (365 nm) and a photodiode sensor was also built and evaluated for the successful automatic detection of explosives and potential application for field-based screening.
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