Adipose stem cell coating of biomimetic β-TCP macrospheres by use of laboratory centrifuge

Chou, J; Green, DW; Singh, K; Hao, J; Ben-Nissan, B; Milthorpe, B

Adipose stem cell coating of biomimetic β-TCP macrospheres by use of laboratory centrifuge

Chou, J

Green, DW Singh, K Hao, J Ben-Nissan, B Milthorpe, B

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Publication Type:: Journal Article
Citation:: BioResearch Open Access, 2013, 2 (1), pp. 67 - 71
Issue Date:: 2013-02-01

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Field	Value	Language
dc.contributor.author	Chou, J https://orcid.org/0000-0002-7472-8016	en_US
dc.contributor.author	Green, DW	en_US
dc.contributor.author	Singh, K	en_US
dc.contributor.author	Hao, J	en_US
dc.contributor.author	Ben-Nissan, B	en_US
dc.contributor.author	Milthorpe, B https://orcid.org/0000-0002-0867-9586	en_US
dc.date.issued	2013-02-01	en_US
dc.identifier.citation	BioResearch Open Access, 2013, 2 (1), pp. 67 - 71	en_US
dc.identifier.uri	http://hdl.handle.net/10453/26497
dc.description.abstract	Biomimetic materials such as coral exoskeletons possess unique architectural structures with a uniform and interconnected porous network that can be beneficial as a scaffold material. In addition, these marine structures can be hydrothermally converted to calcium phosphates, while retaining the original structural properties. The ability of biomaterials to stimulate the local microenvironment is one of the main focuses in tissue engineering, and directly coating the scaffold with stem cells facilitates future potential applications in therapeutics and regenerative medicine. In this article we describe a new and simple method that uses a laboratory centrifuge to coat hydrothermally derived beta-tricalcium phosphate macrospheres from coral exoskeleton with stem cells. In this research the optimal seeding duration and speed were determined to be 1 min and 700 g. Scanning electron micrographs showed complete surface coverage by stem cells within 7 days of seeding. This study constitutes an important step toward achieving functional tissue-engineered implants by increasing our understanding of the influence of dynamic parameters on the efficiency and distribution of stem cell attachment to biomimetic materials and how stem cells interact with biomimetic materials. © Copyright 2013, Mary Ann Liebert, Inc. 2013.	en_US
dc.relation.ispartof	BioResearch Open Access	en_US
dc.relation.isbasedon	10.1089/biores.2012.0269	en_US
dc.title	Adipose stem cell coating of biomimetic β-TCP macrospheres by use of laboratory centrifuge	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	2	en_US
utslib.for	0605 Microbiology	en_US
dc.location.activity	United States
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	open_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	2	en_US

Abstract:

Biomimetic materials such as coral exoskeletons possess unique architectural structures with a uniform and interconnected porous network that can be beneficial as a scaffold material. In addition, these marine structures can be hydrothermally converted to calcium phosphates, while retaining the original structural properties. The ability of biomaterials to stimulate the local microenvironment is one of the main focuses in tissue engineering, and directly coating the scaffold with stem cells facilitates future potential applications in therapeutics and regenerative medicine. In this article we describe a new and simple method that uses a laboratory centrifuge to coat hydrothermally derived beta-tricalcium phosphate macrospheres from coral exoskeleton with stem cells. In this research the optimal seeding duration and speed were determined to be 1 min and 700 g. Scanning electron micrographs showed complete surface coverage by stem cells within 7 days of seeding. This study constitutes an important step toward achieving functional tissue-engineered implants by increasing our understanding of the influence of dynamic parameters on the efficiency and distribution of stem cell attachment to biomimetic materials and how stem cells interact with biomimetic materials. © Copyright 2013, Mary Ann Liebert, Inc. 2013.

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http://hdl.handle.net/10453/26497