First reports of Pseudo-nitzschia micropora and P. hasleana (Bacillariaceae) from the Southern Hemisphere: Morphological, molecular and toxicological characterization

Ajani, P; Murray, S; Hallegraeff, G; Brett, S; Armand, L

First reports of Pseudo-nitzschia micropora and P. hasleana (Bacillariaceae) from the Southern Hemisphere: Morphological, molecular and toxicological characterization

Ajani, P

Murray, S

Hallegraeff, G Brett, S Armand, L

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Publication Type:: Journal Article
Citation:: Phycological Research, 2013, 61 (3), pp. 237 - 248
Issue Date:: 2013-01-01

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Field	Value	Language
dc.contributor.author	Ajani, P https://orcid.org/0000-0001-5364-9936	en_US
dc.contributor.author	Murray, S https://orcid.org/0000-0001-7096-1307	en_US
dc.contributor.author	Hallegraeff, G	en_US
dc.contributor.author	Brett, S	en_US
dc.contributor.author	Armand, L https://orcid.org/0000-0003-3995-308X	en_US
dc.date.issued	2013-01-01	en_US
dc.identifier.citation	Phycological Research, 2013, 61 (3), pp. 237 - 248	en_US
dc.identifier.issn	1322-0829	en_US
dc.identifier.uri	http://hdl.handle.net/10453/26499
dc.description.abstract	Pseudo-nitzschiaH. Peragallo is a marine diatom genus found worldwide in polar, temperate, subtropical and tropical waters. It includes toxigenic representatives that produce domoic acid (DA), a neurotoxin responsible for Amnesic Shellfish Poisoning. In this study we characterized two species of Pseudo-nitzschia collected from Port Stephens and the Hawkesbury River (south eastern Australia) previously unreported from Australian waters. Clonal isolates were sub-sampled for (i) light and transmission electron microscopy; (ii) DNA sequencing, based on the nuclear-encoded partial large subunit ribosomal RNA gene and internal transcribed spacer (ITS)-ITS1, 5.8S and ITS2 rDNA regions and, (iii) DA production as measured by liquid chromatography-mass spectrometry. Morphological and molecular data unambiguously revealed the species to be Pseudo-nitzschia microporaPriisholm, Moestrup & Lundholm (Port Stephens) and Pseudo-nitzschia hasleanaLundholm (Hawkesbury River). This is the first report of the occurrence of these species from the Southern Hemisphere and the first report of P. micropora in warm-temperate waters. Cultures of P. micropora, tested for DA production for the first time, proved to be non-toxic. Similarly, no detectable toxin concentrations were observed for P. hasleana. Species resolution and knowledge on the toxicity of local Pseudo-nitzschia species has important implications for harmful algal bloom monitoring and management. © 2013 Japanese Society of Phycology.	en_US
dc.relation.ispartof	Phycological Research	en_US
dc.relation.isbasedon	10.1111/pre.12020	en_US
dc.subject.classification	Marine Biology & Hydrobiology	en_US
dc.title	First reports of Pseudo-nitzschia micropora and P. hasleana (Bacillariaceae) from the Southern Hemisphere: Morphological, molecular and toxicological characterization	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	61	en_US
utslib.for	0607 Plant Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	61	en_US

Abstract:

Pseudo-nitzschiaH. Peragallo is a marine diatom genus found worldwide in polar, temperate, subtropical and tropical waters. It includes toxigenic representatives that produce domoic acid (DA), a neurotoxin responsible for Amnesic Shellfish Poisoning. In this study we characterized two species of Pseudo-nitzschia collected from Port Stephens and the Hawkesbury River (south eastern Australia) previously unreported from Australian waters. Clonal isolates were sub-sampled for (i) light and transmission electron microscopy; (ii) DNA sequencing, based on the nuclear-encoded partial large subunit ribosomal RNA gene and internal transcribed spacer (ITS)-ITS1, 5.8S and ITS2 rDNA regions and, (iii) DA production as measured by liquid chromatography-mass spectrometry. Morphological and molecular data unambiguously revealed the species to be Pseudo-nitzschia microporaPriisholm, Moestrup & Lundholm (Port Stephens) and Pseudo-nitzschia hasleanaLundholm (Hawkesbury River). This is the first report of the occurrence of these species from the Southern Hemisphere and the first report of P. micropora in warm-temperate waters. Cultures of P. micropora, tested for DA production for the first time, proved to be non-toxic. Similarly, no detectable toxin concentrations were observed for P. hasleana. Species resolution and knowledge on the toxicity of local Pseudo-nitzschia species has important implications for harmful algal bloom monitoring and management. © 2013 Japanese Society of Phycology.

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