The diatom genus Pseudo-nitzschia (Bacillariophyceae) in New South Wales, Australia: Morphotaxonomy, molecular phylogeny, toxicity, and distribution

Ajani, P; Murray, S; Hallegraeff, G; Lundholm, N; Gillings, M; Brett, S; Armand, L

The diatom genus Pseudo-nitzschia (Bacillariophyceae) in New South Wales, Australia: Morphotaxonomy, molecular phylogeny, toxicity, and distribution

Ajani, P

Murray, S

Hallegraeff, G Lundholm, N Gillings, M Brett, S Armand, L

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Publication Type:: Journal Article
Citation:: Journal of Phycology, 2013, 49 (4), pp. 765 - 785
Issue Date:: 2013-08-01

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Field	Value	Language
dc.contributor.author	Ajani, P https://orcid.org/0000-0001-5364-9936	en_US
dc.contributor.author	Murray, S https://orcid.org/0000-0001-7096-1307	en_US
dc.contributor.author	Hallegraeff, G	en_US
dc.contributor.author	Lundholm, N	en_US
dc.contributor.author	Gillings, M	en_US
dc.contributor.author	Brett, S	en_US
dc.contributor.author	Armand, L https://orcid.org/0000-0003-3995-308X	en_US
dc.date.available	2013-05-19	en_US
dc.date.issued	2013-08-01	en_US
dc.identifier.citation	Journal of Phycology, 2013, 49 (4), pp. 765 - 785	en_US
dc.identifier.issn	0022-3646	en_US
dc.identifier.uri	http://hdl.handle.net/10453/26500
dc.description.abstract	Species belonging to the potentially harmful diatom genus Pseudo-nitzschia, isolated from 16 localities (31 sampling events) in the coastal waters of south-eastern Australia, were examined. Clonal isolates were characterized by (i) light and transmission electron microscopy; (ii) phylogenies, based on sequencing of nuclear-encoded ribosomal deoxyribonucleic acid (rDNA) regions and, (iii) domoic acid (DA) production as measured by liquid chromatography-mass spectrometry (LC-MS/MS). Ten taxa were unequivocally confirmed as Pseudo-nitzschia americana, P. arenysensis, P. calliantha, P. cuspidata, P. fraudulenta, P. hasleana, P. micropora, P. multiseries, P. multistriata, and P. pungens. An updated taxonomic key for south-eastern Australian Pseudo-nitzschia is presented. The occurrence of two toxigenic species, P. multistriata (maximum concentration 11 pg DA per cell) and P. cuspidata (25.4 pg DA per cell), was documented for the first time in Australia. The Australian strains of P. multiseries, a consistent producer of DA in strains throughout the world, were nontoxic. Data from 5,888 water samples, collected from 31 oyster-growing estuaries (2,000 km coastline) from 2005 to 2009, revealed 310 regulatory exceedances for "Total Pseudo-nitzschia," resulting in six toxic episodes. Further examination of high-risk estuaries revealed that the "P. seriata group" had highest cell densities in the austral summer, autumn, or spring (species dependent), and lowest cell densities in the austral winter, while the "P. delicatissima group" had highest in winter and spring. © 2013 Phycological Society of America.	en_US
dc.relation.ispartof	Journal of Phycology	en_US
dc.relation.isbasedon	10.1111/jpy.12087	en_US
dc.subject.classification	Marine Biology & Hydrobiology	en_US
dc.title	The diatom genus Pseudo-nitzschia (Bacillariophyceae) in New South Wales, Australia: Morphotaxonomy, molecular phylogeny, toxicity, and distribution	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.citation.volume	49	en_US
utslib.for	0607 Plant Biology	en_US
utslib.for	0704 Fisheries Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	4	en_US
pubs.publication-status	Published	en_US
pubs.volume	49	en_US

Abstract:

Species belonging to the potentially harmful diatom genus Pseudo-nitzschia, isolated from 16 localities (31 sampling events) in the coastal waters of south-eastern Australia, were examined. Clonal isolates were characterized by (i) light and transmission electron microscopy; (ii) phylogenies, based on sequencing of nuclear-encoded ribosomal deoxyribonucleic acid (rDNA) regions and, (iii) domoic acid (DA) production as measured by liquid chromatography-mass spectrometry (LC-MS/MS). Ten taxa were unequivocally confirmed as Pseudo-nitzschia americana, P. arenysensis, P. calliantha, P. cuspidata, P. fraudulenta, P. hasleana, P. micropora, P. multiseries, P. multistriata, and P. pungens. An updated taxonomic key for south-eastern Australian Pseudo-nitzschia is presented. The occurrence of two toxigenic species, P. multistriata (maximum concentration 11 pg DA per cell) and P. cuspidata (25.4 pg DA per cell), was documented for the first time in Australia. The Australian strains of P. multiseries, a consistent producer of DA in strains throughout the world, were nontoxic. Data from 5,888 water samples, collected from 31 oyster-growing estuaries (2,000 km coastline) from 2005 to 2009, revealed 310 regulatory exceedances for "Total Pseudo-nitzschia," resulting in six toxic episodes. Further examination of high-risk estuaries revealed that the "P. seriata group" had highest cell densities in the austral summer, autumn, or spring (species dependent), and lowest cell densities in the austral winter, while the "P. delicatissima group" had highest in winter and spring. © 2013 Phycological Society of America.

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