Application of an ELISA-type screen printed electrode-based potentiometric assay to the detection of Cryptosporidium parvum oocysts

Publisher:
Elsevier
Publication Type:
Journal Article
Citation:
Journal of Microbiological Methods, 2013, 95 (2), pp. 182 - 185
Issue Date:
2013-01
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We report a novel electrochemical method for the rapid detection of the parasitic protozoan, Cryptosporidium parvum. An antibody-based capture format was transferred onto screen-printed electrodes and the presence of horseradish peroxidase-labelled antibodies binding to the oocystswas potentiometrically detected. Thismethod allowed the detection of 5 × 102 Cryptosporidium oocysts per mL in 60 min.
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