Microsatellite primer development for the seagrass Zostera nigricaulis (Zosteraceae)

Smith, TM; York, PH; Stanley, AM; Macreadie, PI; Keough, MJ; Ross, DJ; Sherman, CDH

Microsatellite primer development for the seagrass Zostera nigricaulis (Zosteraceae)

Smith, TM York, PH Stanley, AM Macreadie, PI

Keough, MJ Ross, DJ Sherman, CDH

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Publication Type:: Journal Article
Citation:: Conservation Genetics Resources, 2013, 5 (3), pp. 607 - 610
Issue Date:: 2013-09-01

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Field	Value	Language
dc.contributor.author	Smith, TM	en_US
dc.contributor.author	York, PH	en_US
dc.contributor.author	Stanley, AM	en_US
dc.contributor.author	Macreadie, PI https://orcid.org/0000-0001-7362-0882	en_US
dc.contributor.author	Keough, MJ	en_US
dc.contributor.author	Ross, DJ	en_US
dc.contributor.author	Sherman, CDH	en_US
dc.date.issued	2013-09-01	en_US
dc.identifier.citation	Conservation Genetics Resources, 2013, 5 (3), pp. 607 - 610	en_US
dc.identifier.issn	1877-7252	en_US
dc.identifier.uri	http://hdl.handle.net/10453/26541
dc.description.abstract	Seagrasses are marine angiosperms with a worldwide distribution that form conspicuous beds in nearshore habitats. Despite being universally recognised as a foundation species that performs a number of important ecosystems functions (incl. sediment stabilisation, facilitation of biodiversity, nutrient cycling and carbon sequestration), global seagrass habitats are in decline. Resilience-the ability to recover from disturbance without switching to an alternative state-is paramount to the maintenance and persistence of seagrass habitats. Genetic diversity is a key component of seagrass resilience and contributes to an understanding of population structure, connectivity between populations, and reproductive strategies. Microsatellite primers were developed to investigate the resilience of the seagrass Zostera nigricaulis, which dominates subtidal habitats in the bays of south-eastern Australia. We also tested for cross-amplification of markers between Z. nigricaulis and previously developed markers for the sympatric species Z. muelleri to assess their applicability for use in assessing patterns of genetic diversity, population structure, and mating system. Using next-generation sequencing we isolated 11 novel microsatellite loci for Z. nigricaulis, 8 of which were polymorphic for the samples tested. Allelic diversity ranged from 1 to 8. None of the primer pairs developed for Z. nigricaulis cross-amplified in Z. muelleri; but 14 of 24 primer pairs previously developed for Z. muelleri amplified clearly in Z. nigricaulis samples with six of these showing polymorphism. The results demonstrate the applicability of the Z. nigricaulis microsatellite primers for use in the study of population genetics and limited cross-amplification with Z. muelleri. © 2013 Springer Science+Business Media Dordrecht.	en_US
dc.relation.ispartof	Conservation Genetics Resources	en_US
dc.relation.isbasedon	10.1007/s12686-013-9862-3	en_US
dc.title	Microsatellite primer development for the seagrass Zostera nigricaulis (Zosteraceae)	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	5	en_US
utslib.for	0604 Genetics	en_US
utslib.for	0603 Evolutionary Biology	en_US
utslib.for	0704 Fisheries Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	5	en_US

Abstract:

Seagrasses are marine angiosperms with a worldwide distribution that form conspicuous beds in nearshore habitats. Despite being universally recognised as a foundation species that performs a number of important ecosystems functions (incl. sediment stabilisation, facilitation of biodiversity, nutrient cycling and carbon sequestration), global seagrass habitats are in decline. Resilience-the ability to recover from disturbance without switching to an alternative state-is paramount to the maintenance and persistence of seagrass habitats. Genetic diversity is a key component of seagrass resilience and contributes to an understanding of population structure, connectivity between populations, and reproductive strategies. Microsatellite primers were developed to investigate the resilience of the seagrass Zostera nigricaulis, which dominates subtidal habitats in the bays of south-eastern Australia. We also tested for cross-amplification of markers between Z. nigricaulis and previously developed markers for the sympatric species Z. muelleri to assess their applicability for use in assessing patterns of genetic diversity, population structure, and mating system. Using next-generation sequencing we isolated 11 novel microsatellite loci for Z. nigricaulis, 8 of which were polymorphic for the samples tested. Allelic diversity ranged from 1 to 8. None of the primer pairs developed for Z. nigricaulis cross-amplified in Z. muelleri; but 14 of 24 primer pairs previously developed for Z. muelleri amplified clearly in Z. nigricaulis samples with six of these showing polymorphism. The results demonstrate the applicability of the Z. nigricaulis microsatellite primers for use in the study of population genetics and limited cross-amplification with Z. muelleri. © 2013 Springer Science+Business Media Dordrecht.

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http://hdl.handle.net/10453/26541