Effect of contact lens wear on the diurnal profile of matrix metalloproteinase 9 in tears

Markoulli, M; Papas, E; Cole, N; Holden, B

Effect of contact lens wear on the diurnal profile of matrix metalloproteinase 9 in tears

Markoulli, M Papas, E Cole, N

Holden, B

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Publication Type:: Journal Article
Citation:: Optometry and Vision Science, 2013, 90 (5), pp. 419 - 429
Issue Date:: 2013-05-01

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Field	Value	Language
dc.contributor.author	Markoulli, M	en_US
dc.contributor.author	Papas, E	en_US
dc.contributor.author	Cole, N https://orcid.org/0000-0002-9866-4694	en_US
dc.contributor.author	Holden, B	en_US
dc.date.issued	2013-05-01	en_US
dc.identifier.citation	Optometry and Vision Science, 2013, 90 (5), pp. 419 - 429	en_US
dc.identifier.issn	1040-5488	en_US
dc.identifier.uri	http://hdl.handle.net/10453/27304
dc.description.abstract	Purpose: This study set out to determine how contact lens wear affects the profile of matrix metalloproteinase 9 (MMP-9) in the tear film. Methods: Flush tears were collected from 47 healthy neophytes before lens wear, during the first day of lens wear, and after 1 month adaptation. Participants were randomized to either Acuvue Oasys or O2OPTIX with the choice of extended (EW) or daily wear (DW). Each time, tears were collected at midday, before sleep, and on waking and analyzed for concentrations of total protein, MMP-9, and its regulators TIMP-1 (tissue inhibitor of metalloproteinases 1) and NGAL (neutrophil gelatinase-associated lipocalin). Results: Initial extended contact lens wear resulted in significantly elevated MMP-9 levels on waking (3598.7 ± 3229.1 ng/mL) compared with the same time point at baseline (2123.3 ± 1762.8; p = 0.02), whereas DW remained unchanged (2373.0 ± 2091.6 ng/mL; p = 0.61). After 1 month of EW, the levels on awakening were no longer different to those of baseline (2408.2 ± 1376.1 ng/mL; p = 0.63). The MMP:TIMP ratio during EW was greater after the first night (18.6 ± 19.9) than both no wear (13.2 ± 13.4) and 1 month (10.4 ± 7.7), but only the latter was significant (p = 0.048). The profile of NGAL did not differ from baseline (p = 0.055). Conclusions: In the neophyte, the initial period of overnight lens wear seems to disturb the tear film homeostasis, as indicated by a significant increase in MMP-9 on awakening. The return to baseline by 1 month suggests that an adaptive process takes place. No comparable changes are seen in DW. © 2013 American Academy of Optometry.	en_US
dc.relation.ispartof	Optometry and Vision Science	en_US
dc.relation.isbasedon	10.1097/OPX.0b013e31828d7d3b	en_US
dc.subject.classification	Ophthalmology & Optometry	en_US
dc.subject.mesh	Tears	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Myopia	en_US
dc.subject.mesh	Tissue Inhibitor of Metalloproteinase-1	en_US
dc.subject.mesh	Follow-Up Studies	en_US
dc.subject.mesh	Prospective Studies	en_US
dc.subject.mesh	Equipment Design	en_US
dc.subject.mesh	Contact Lenses	en_US
dc.subject.mesh	Circadian Rhythm	en_US
dc.subject.mesh	Adolescent	en_US
dc.subject.mesh	Adult	en_US
dc.subject.mesh	Middle Aged	en_US
dc.subject.mesh	Female	en_US
dc.subject.mesh	Male	en_US
dc.subject.mesh	Matrix Metalloproteinase 9	en_US
dc.subject.mesh	Young Adult	en_US
dc.title	Effect of contact lens wear on the diurnal profile of matrix metalloproteinase 9 in tears	en_US
dc.type	Journal Article
utslib.citation.volume	5	en_US
utslib.citation.volume	90	en_US
utslib.for	1113 Opthalmology and Optometry	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	closed_access
pubs.issue	5	en_US
pubs.publication-status	Published	en_US
pubs.volume	90	en_US

Abstract:

Purpose: This study set out to determine how contact lens wear affects the profile of matrix metalloproteinase 9 (MMP-9) in the tear film. Methods: Flush tears were collected from 47 healthy neophytes before lens wear, during the first day of lens wear, and after 1 month adaptation. Participants were randomized to either Acuvue Oasys or O2OPTIX with the choice of extended (EW) or daily wear (DW). Each time, tears were collected at midday, before sleep, and on waking and analyzed for concentrations of total protein, MMP-9, and its regulators TIMP-1 (tissue inhibitor of metalloproteinases 1) and NGAL (neutrophil gelatinase-associated lipocalin). Results: Initial extended contact lens wear resulted in significantly elevated MMP-9 levels on waking (3598.7 ± 3229.1 ng/mL) compared with the same time point at baseline (2123.3 ± 1762.8; p = 0.02), whereas DW remained unchanged (2373.0 ± 2091.6 ng/mL; p = 0.61). After 1 month of EW, the levels on awakening were no longer different to those of baseline (2408.2 ± 1376.1 ng/mL; p = 0.63). The MMP:TIMP ratio during EW was greater after the first night (18.6 ± 19.9) than both no wear (13.2 ± 13.4) and 1 month (10.4 ± 7.7), but only the latter was significant (p = 0.048). The profile of NGAL did not differ from baseline (p = 0.055). Conclusions: In the neophyte, the initial period of overnight lens wear seems to disturb the tear film homeostasis, as indicated by a significant increase in MMP-9 on awakening. The return to baseline by 1 month suggests that an adaptive process takes place. No comparable changes are seen in DW. © 2013 American Academy of Optometry.

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http://hdl.handle.net/10453/27304