Potential of proteins and their expression level in marine phytoplankton (Prymnesium parvum) as biomarker of N, P and Fe conditions in aquatic systems

Hasegawa, H; Rahman, MM; Kato, S; Maki, T; Rahman, MA

Potential of proteins and their expression level in marine phytoplankton (Prymnesium parvum) as biomarker of N, P and Fe conditions in aquatic systems

Hasegawa, H Rahman, MM Kato, S Maki, T Rahman, MA

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Publisher:: Scientific Research Publishing
Publication Type:: Journal Article
Citation:: Advances in Biological Chemistry, 2013, 3 (3), pp. 338 - 346
Issue Date:: 2013-01

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Field	Value	Language
dc.contributor.author	Hasegawa, H	en_US
dc.contributor.author	Rahman, MM	en_US
dc.contributor.author	Kato, S	en_US
dc.contributor.author	Maki, T	en_US
dc.contributor.author	Rahman, MA https://orcid.org/0000-0002-8065-0286	en_US
dc.date.issued	2013-01	en_US
dc.identifier.citation	Advances in Biological Chemistry, 2013, 3 (3), pp. 338 - 346	en_US
dc.identifier.issn	2162-2191	en_US
dc.identifier.uri	http://hdl.handle.net/10453/27466
dc.description.abstract	Nitrogen (N), phosphorus (P) and Iron (Fe) are im-portant nutrients for phytoplankton, and are key limiting nutrients in many marine systems. In the present study, growth and protein expression of ma-rine phytoplankton Prymnesium parvum under dif-ferent nitrate, phosphate and iron conditions were investigated in order to evaluate whether proteins and their expression level can be used as biomarker of N, P, and Fe conditions in aquatic systems. The growth of P. parvum increased with the increase of nitrate, phosphate and iron concentrations in the culture medium. Protein expression levels also differed significantly (p < 0.001) for different nitrate, phosphate and iron conditions in the culture medium. The expression level of an 83 kDa protein at 0 and 5 µM nitrate treatments differed significantly (p < 0.001) from those at 20, 30, 50 and 100 µM nitrate treatments, indicating the expression levels of this protein as a biomarker of N status in the culture me-dium. A 121 kDa protein was up-regulated at phos-phate stress conditions ([P] = 1.0 µM), while this pro-tein was not expressed at phosphate replete conditions ([P] = 5 µM). Therefore, the expression of 121 kDa protein in P. parvum is indicative of phosphate replete condition in aquatic systems. The expression level of a 42 kDa was significantly higher (p < 0.01) at Fe-stress condition ([Fe] = 0.01 µM) than Fe-replete conditions ([Fe] = 0.1 µM). In addition, a new protein of 103 kDa was only expressed under Fe-deplete condition ([Fe] = 0.01 µM). Therefore, the 42 and 103 kDa proteins can be used as a biomarker of Fe-limitation condition of aquatic systems. However, further studies (two dimensional gel electrophoresis and mass spectrometry) are needed to identify and characterize these proteins in P. parvum.	en_US
dc.publisher	Scientific Research Publishing	en_US
dc.relation.ispartof	Advances in Biological Chemistry	en_US
dc.relation.isbasedon	10.4236/abc.2013.33038	en_US
dc.title	Potential of proteins and their expression level in marine phytoplankton (Prymnesium parvum) as biomarker of N, P and Fe conditions in aquatic systems	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.for	0602 Ecology	en_US
dc.location.activity	Sydney, Australia
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access
pubs.consider-herdc	true	en_US
pubs.issue	3	en_US
pubs.volume	3	en_US

Abstract:

Nitrogen (N), phosphorus (P) and Iron (Fe) are im-portant nutrients for phytoplankton, and are key limiting nutrients in many marine systems. In the present study, growth and protein expression of ma-rine phytoplankton Prymnesium parvum under dif-ferent nitrate, phosphate and iron conditions were investigated in order to evaluate whether proteins and their expression level can be used as biomarker of N, P, and Fe conditions in aquatic systems. The growth of P. parvum increased with the increase of nitrate, phosphate and iron concentrations in the culture medium. Protein expression levels also differed significantly (p < 0.001) for different nitrate, phosphate and iron conditions in the culture medium. The expression level of an 83 kDa protein at 0 and 5 µM nitrate treatments differed significantly (p < 0.001) from those at 20, 30, 50 and 100 µM nitrate treatments, indicating the expression levels of this protein as a biomarker of N status in the culture me-dium. A 121 kDa protein was up-regulated at phos-phate stress conditions ([P] = 1.0 µM), while this pro-tein was not expressed at phosphate replete conditions ([P] = 5 µM). Therefore, the expression of 121 kDa protein in P. parvum is indicative of phosphate replete condition in aquatic systems. The expression level of a 42 kDa was significantly higher (p < 0.01) at Fe-stress condition ([Fe] = 0.01 µM) than Fe-replete conditions ([Fe] = 0.1 µM). In addition, a new protein of 103 kDa was only expressed under Fe-deplete condition ([Fe] = 0.01 µM). Therefore, the 42 and 103 kDa proteins can be used as a biomarker of Fe-limitation condition of aquatic systems. However, further studies (two dimensional gel electrophoresis and mass spectrometry) are needed to identify and characterize these proteins in P. parvum.

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