A conserved acetyl esterase domain targets diverse bacteriophages to the Vi capsular receptor of Salmonella enterica serovar typhi

Pickard, D; Toribio, AL; Petty, NK; Van Tonder, A; Yu, L; Goulding, D; Barrell, B; Rance, R; Harris, D; Wetter, M; Wain, J; Choudhary, J; Thomson, N; Dougan, G

A conserved acetyl esterase domain targets diverse bacteriophages to the Vi capsular receptor of Salmonella enterica serovar typhi

Pickard, D Toribio, AL Petty, NK

Van Tonder, A Yu, L Goulding, D Barrell, B Rance, R Harris, D Wetter, M Wain, J Choudhary, J Thomson, N Dougan, G

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Publication Type:: Journal Article
Citation:: Journal of Bacteriology, 2010, 192 (21), pp. 5746 - 5754
Issue Date:: 2010-01-01

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Field	Value	Language
dc.contributor.author	Pickard, D	en_US
dc.contributor.author	Toribio, AL	en_US
dc.contributor.author	Petty, NK https://orcid.org/0000-0001-6528-9886	en_US
dc.contributor.author	Van Tonder, A	en_US
dc.contributor.author	Yu, L	en_US
dc.contributor.author	Goulding, D	en_US
dc.contributor.author	Barrell, B	en_US
dc.contributor.author	Rance, R	en_US
dc.contributor.author	Harris, D	en_US
dc.contributor.author	Wetter, M	en_US
dc.contributor.author	Wain, J	en_US
dc.contributor.author	Choudhary, J	en_US
dc.contributor.author	Thomson, N	en_US
dc.contributor.author	Dougan, G	en_US
dc.date.issued	2010-01-01	en_US
dc.identifier.citation	Journal of Bacteriology, 2010, 192 (21), pp. 5746 - 5754	en_US
dc.identifier.issn	0021-9193	en_US
dc.identifier.uri	http://hdl.handle.net/10453/27801
dc.description.abstract	A number of bacteriophages have been identified that target the Vi capsular antigen of Salmonella enterica serovar Typhi. Here we show that these Vi phages represent a remarkably diverse set of phages belonging to three phage families, including Podoviridae and Myoviridae. Genome analysis facilitated the further classifi-cation of these phages and highlighted aspects of their independent evolution. Significantly, a conserved protein domain carrying an acetyl esterase was found to be associated with at least one tail fiber gene for all Vi phages, and the presence of this domain was confirmed in representative phage particles by mass spectrometric analysis. Thus, we provide a simple explanation and paradigm of how a diverse group of phages target a single key virulence antigen associated with this important human-restricted pathogen. Copyright © 2010, American Society for Microbiology. All Rights Reserved.	en_US
dc.relation.ispartof	Journal of Bacteriology	en_US
dc.relation.isbasedon	10.1128/JB.00659-10	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Salmonella typhi	en_US
dc.subject.mesh	Salmonella Phages	en_US
dc.subject.mesh	Acetylesterase	en_US
dc.subject.mesh	Polysaccharides, Bacterial	en_US
dc.subject.mesh	Viral Proteins	en_US
dc.subject.mesh	Gene Expression Regulation, Viral	en_US
dc.subject.mesh	Protein Structure, Tertiary	en_US
dc.subject.mesh	Synteny	en_US
dc.subject.mesh	Genome, Viral	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.title	A conserved acetyl esterase domain targets diverse bacteriophages to the Vi capsular receptor of Salmonella enterica serovar typhi	en_US
dc.type	Journal Article
utslib.citation.volume	21	en_US
utslib.citation.volume	192	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	21	en_US
pubs.publication-status	Published	en_US
pubs.volume	192	en_US

Abstract:

A number of bacteriophages have been identified that target the Vi capsular antigen of Salmonella enterica serovar Typhi. Here we show that these Vi phages represent a remarkably diverse set of phages belonging to three phage families, including Podoviridae and Myoviridae. Genome analysis facilitated the further classifi-cation of these phages and highlighted aspects of their independent evolution. Significantly, a conserved protein domain carrying an acetyl esterase was found to be associated with at least one tail fiber gene for all Vi phages, and the presence of this domain was confirmed in representative phage particles by mass spectrometric analysis. Thus, we provide a simple explanation and paradigm of how a diverse group of phages target a single key virulence antigen associated with this important human-restricted pathogen. Copyright © 2010, American Society for Microbiology. All Rights Reserved.

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http://hdl.handle.net/10453/27801