A commensal gone bad: Complete genome sequence of the prototypical enterotoxigenic Escherichia coli strain H10407

Crossman, LC; Chaudhuri, RR; Beatson, SA; Wells, TJ; Desvaux, M; Cunningham, AF; Petty, NK; Mahon, V; Brinkley, C; Hobman, JL; Savarino, SJ; Turner, SM; Pallen, MJ; Penn, CW; Parkhill, J; Turner, AK; Johnson, TJ; Thomson, NR; Smith, SGJ; Henderson, IR

A commensal gone bad: Complete genome sequence of the prototypical enterotoxigenic Escherichia coli strain H10407

Crossman, LC Chaudhuri, RR Beatson, SA Wells, TJ Desvaux, M Cunningham, AF Petty, NK

Mahon, V Brinkley, C Hobman, JL Savarino, SJ Turner, SM Pallen, MJ Penn, CW Parkhill, J Turner, AK Johnson, TJ Thomson, NR Smith, SGJ Henderson, IR

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Publication Type:: Journal Article
Citation:: Journal of Bacteriology, 2010, 192 (21), pp. 5822 - 5831
Issue Date:: 2010-11-01

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Field	Value	Language
dc.contributor.author	Crossman, LC	en_US
dc.contributor.author	Chaudhuri, RR	en_US
dc.contributor.author	Beatson, SA	en_US
dc.contributor.author	Wells, TJ	en_US
dc.contributor.author	Desvaux, M	en_US
dc.contributor.author	Cunningham, AF	en_US
dc.contributor.author	Petty, NK https://orcid.org/0000-0001-6528-9886	en_US
dc.contributor.author	Mahon, V	en_US
dc.contributor.author	Brinkley, C	en_US
dc.contributor.author	Hobman, JL	en_US
dc.contributor.author	Savarino, SJ	en_US
dc.contributor.author	Turner, SM	en_US
dc.contributor.author	Pallen, MJ	en_US
dc.contributor.author	Penn, CW	en_US
dc.contributor.author	Parkhill, J	en_US
dc.contributor.author	Turner, AK	en_US
dc.contributor.author	Johnson, TJ	en_US
dc.contributor.author	Thomson, NR	en_US
dc.contributor.author	Smith, SGJ	en_US
dc.contributor.author	Henderson, IR	en_US
dc.date.issued	2010-11-01	en_US
dc.identifier.citation	Journal of Bacteriology, 2010, 192 (21), pp. 5822 - 5831	en_US
dc.identifier.issn	0021-9193	en_US
dc.identifier.uri	http://hdl.handle.net/10453/27802
dc.description.abstract	In most cases, Escherichia coli exists as a harmless commensal organism, but it may on occasion cause intestinal and/or extraintestinal disease. Enterotoxigenic E. coli (ETEC) is the predominant cause of E. coli-mediated diarrhea in the developing world and is responsible for a significant portion of pediatric deaths. In this study, we determined the complete genomic sequence of E. coli H10407, a prototypical strain of enterotoxigenic E. coli, which reproducibly elicits diarrhea in human volunteer studies. We performed genomic and phylogenetic comparisons with other E. coli strains, revealing that the chromosome is closely related to that of the nonpathogenic commensal strain E. coli HS and to those of the laboratory strains E. coli K-12 and C. Furthermore, these analyses demonstrated that there were no chromosomally encoded factors unique to any sequenced ETEC strains. Comparison of the E. coli H10407 plasmids with those from several ETEC strains revealed that the plasmids had a mosaic structure but that several loci were conserved among ETEC strains. This study provides a genetic context for the vast amount of experimental and epidemiological data that have been published. Copyright © 2010, American Society for Microbiology. All Rights Reserved.	en_US
dc.relation.ispartof	Journal of Bacteriology	en_US
dc.relation.isbasedon	10.1128/JB.00710-10	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Chromosomes, Bacterial	en_US
dc.subject.mesh	Fimbriae Proteins	en_US
dc.subject.mesh	Escherichia coli Proteins	en_US
dc.subject.mesh	Gene Expression Profiling	en_US
dc.subject.mesh	Gene Expression Regulation, Bacterial	en_US
dc.subject.mesh	Amino Acid Sequence	en_US
dc.subject.mesh	Genome, Bacterial	en_US
dc.subject.mesh	Plasmids	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.subject.mesh	Enterotoxigenic Escherichia coli	en_US
dc.title	A commensal gone bad: Complete genome sequence of the prototypical enterotoxigenic Escherichia coli strain H10407	en_US
dc.type	Journal Article
utslib.citation.volume	21	en_US
utslib.citation.volume	192	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	21	en_US
pubs.publication-status	Published	en_US
pubs.volume	192	en_US

Abstract:

In most cases, Escherichia coli exists as a harmless commensal organism, but it may on occasion cause intestinal and/or extraintestinal disease. Enterotoxigenic E. coli (ETEC) is the predominant cause of E. coli-mediated diarrhea in the developing world and is responsible for a significant portion of pediatric deaths. In this study, we determined the complete genomic sequence of E. coli H10407, a prototypical strain of enterotoxigenic E. coli, which reproducibly elicits diarrhea in human volunteer studies. We performed genomic and phylogenetic comparisons with other E. coli strains, revealing that the chromosome is closely related to that of the nonpathogenic commensal strain E. coli HS and to those of the laboratory strains E. coli K-12 and C. Furthermore, these analyses demonstrated that there were no chromosomally encoded factors unique to any sequenced ETEC strains. Comparison of the E. coli H10407 plasmids with those from several ETEC strains revealed that the plasmids had a mosaic structure but that several loci were conserved among ETEC strains. This study provides a genetic context for the vast amount of experimental and epidemiological data that have been published. Copyright © 2010, American Society for Microbiology. All Rights Reserved.

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http://hdl.handle.net/10453/27802