Identical genotypes of an ericoid mycorrhiza-forming fungus occur in roots of Epacris pulchella (Ericaceae) and Leptospermum polygalifolium (Myrtaceae) in an Australian sclerophyll forest

Curlevski, NJA; Chambers, SM; Anderson, IC; Cairney, JWG

Identical genotypes of an ericoid mycorrhiza-forming fungus occur in roots of Epacris pulchella (Ericaceae) and Leptospermum polygalifolium (Myrtaceae) in an Australian sclerophyll forest

Curlevski, NJA Chambers, SM Anderson, IC Cairney, JWG

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Publication Type:: Journal Article
Citation:: FEMS Microbiology Ecology, 2009, 67 (3), pp. 411 - 420
Issue Date:: 2009-03-01

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Field	Value	Language
dc.contributor.author	Curlevski, NJA	en_US
dc.contributor.author	Chambers, SM	en_US
dc.contributor.author	Anderson, IC	en_US
dc.contributor.author	Cairney, JWG	en_US
dc.date.issued	2009-03-01	en_US
dc.identifier.citation	FEMS Microbiology Ecology, 2009, 67 (3), pp. 411 - 420	en_US
dc.identifier.issn	0168-6496	en_US
dc.identifier.uri	http://hdl.handle.net/10453/28206
dc.description.abstract	Assemblages of fungi associated with roots of cooccurring Epacris pulchella (Ericaceae) and Leptospermum polygalifolium (Myrtaceae) seedlings at a sclerophyll forest site in New South Wales, Australia, were investigated by direct DNA extraction and analysis of rRNA gene internal transcribed spacer (ITS) products by denaturing gradient gel electrophoresis (DGGE) and terminal restriction fragment length polymorphism (T-RFLP) analyses. While ordination of the DGGE data suggested that the assemblages did not differ significantly between the two plant taxa, T-RFLP data provided marginal statistical support for the presence of different assemblages. Fungi isolated from roots of both plants were identified by ITS sequence comparisons largely as ascomycetes, several of which had close sequence identity to Helotiales ericoid mycorrhizal (ERM) fungi. One isolate morphotype from E. pulchella had close sequence similarity to ectomycorrhizal fungi in the Cenococcum geophilum complex, and neighbour-joining analysis grouped this strongly with other Australian C. geophilum-like sequences. Distribution of genotypes of an ERM Helotiales ascomycete in root systems of the two plant taxa was also investigated using inter-simple sequence repeat (ISSR)-PCR. Nineteen ISSR genotypes were identified, two of which were present in roots of both plant taxa. The results are discussed in the context of potential mycelial connections between Ericaceae and non-Ericaceae plants. © 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.	en_US
dc.relation.ispartof	FEMS Microbiology Ecology	en_US
dc.relation.isbasedon	10.1111/j.1574-6941.2008.00637.x	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Mycorrhizae	en_US
dc.subject.mesh	Ericaceae	en_US
dc.subject.mesh	Myrtaceae	en_US
dc.subject.mesh	Plant Roots	en_US
dc.subject.mesh	Trees	en_US
dc.subject.mesh	DNA, Fungal	en_US
dc.subject.mesh	DNA, Ribosomal Spacer	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Soil Microbiology	en_US
dc.subject.mesh	Biodiversity	en_US
dc.subject.mesh	Genotype	en_US
dc.subject.mesh	Polymorphism, Restriction Fragment Length	en_US
dc.subject.mesh	New South Wales	en_US
dc.title	Identical genotypes of an ericoid mycorrhiza-forming fungus occur in roots of Epacris pulchella (Ericaceae) and Leptospermum polygalifolium (Myrtaceae) in an Australian sclerophyll forest	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	67	en_US
utslib.for	0502 Environmental Science and Management	en_US
utslib.for	05 Environmental Sciences	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	67	en_US

Abstract:

Assemblages of fungi associated with roots of cooccurring Epacris pulchella (Ericaceae) and Leptospermum polygalifolium (Myrtaceae) seedlings at a sclerophyll forest site in New South Wales, Australia, were investigated by direct DNA extraction and analysis of rRNA gene internal transcribed spacer (ITS) products by denaturing gradient gel electrophoresis (DGGE) and terminal restriction fragment length polymorphism (T-RFLP) analyses. While ordination of the DGGE data suggested that the assemblages did not differ significantly between the two plant taxa, T-RFLP data provided marginal statistical support for the presence of different assemblages. Fungi isolated from roots of both plants were identified by ITS sequence comparisons largely as ascomycetes, several of which had close sequence identity to Helotiales ericoid mycorrhizal (ERM) fungi. One isolate morphotype from E. pulchella had close sequence similarity to ectomycorrhizal fungi in the Cenococcum geophilum complex, and neighbour-joining analysis grouped this strongly with other Australian C. geophilum-like sequences. Distribution of genotypes of an ERM Helotiales ascomycete in root systems of the two plant taxa was also investigated using inter-simple sequence repeat (ISSR)-PCR. Nineteen ISSR genotypes were identified, two of which were present in roots of both plant taxa. The results are discussed in the context of potential mycelial connections between Ericaceae and non-Ericaceae plants. © 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

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