Diversity of soil and rhizosphere fungi under Araucaria bidwillii (Bunya pine) at an Australian tropical montane rainforest site

Curlevski, NJA; Xu, ZH; Anderson, IC; Cairney, JWG

Diversity of soil and rhizosphere fungi under Araucaria bidwillii (Bunya pine) at an Australian tropical montane rainforest site

Curlevski, NJA Xu, ZH Anderson, IC Cairney, JWG

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Publication Type:: Journal Article
Citation:: Fungal Diversity, 2010, 40 pp. 12 - 22
Issue Date:: 2010-01-01

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Field	Value	Language
dc.contributor.author	Curlevski, NJA	en_US
dc.contributor.author	Xu, ZH	en_US
dc.contributor.author	Anderson, IC	en_US
dc.contributor.author	Cairney, JWG	en_US
dc.date.issued	2010-01-01	en_US
dc.identifier.citation	Fungal Diversity, 2010, 40 pp. 12 - 22	en_US
dc.identifier.issn	1560-2745	en_US
dc.identifier.uri	http://hdl.handle.net/10453/28221
dc.description.abstract	Abstract Samples of rhizosphere soil and bulk soil were collected from under Araucaria bidwillii in a tropical montane rainforest at Mount Lewis forest reserve, Queensland, Australia. DNA was extracted from soil and denaturing gradient gel electrophoresis (DGGE) profiles of PCRamplified partial rDNA internal transcribed spacer (ITS) regions of rhizosphere and bulk soil samples compared. Neither canonical analysis of the principal coordinates nor principal component analysis revealed clear separation of soil samples according to their origin in rhizosphere or bulk soil. Following amplification of rDNA ITS regions using the primers ITS1-F and ITS4, restriction fragment length polymorphism (RFLP) and sequence analyses were also used to identify fungal sequences that were present in clone assemblages from rhizosphere soil and bulk soil. A total of fifty-three RFLP types were thus identified, with the majority being ascomycetes and the remainder basidiomycetes or zygomycetes. Nine RFLP types had affinity with Mortierellaceae, and phylogenetic analysis indicated that these probably represent multiple Mortierella species in both rhizosphere and bulk soil samples. Similar evidence was obtained for the presence of multiple Trichosporon species. RFLP type 53 from a bulk soil sample was identified as a Soil Clone Group I ascomycete, confirming the widespread occurrence of this group. © Kevin D. Hyde 2009.	en_US
dc.relation.ispartof	Fungal Diversity	en_US
dc.relation.isbasedon	10.1007/s13225-009-0001-0	en_US
dc.subject.classification	Mycology & Parasitology	en_US
dc.title	Diversity of soil and rhizosphere fungi under Araucaria bidwillii (Bunya pine) at an Australian tropical montane rainforest site	en_US
dc.type	Journal Article
utslib.citation.volume	40	en_US
utslib.for	0607 Plant Biology	en_US
utslib.for	0603 Evolutionary Biology	en_US
utslib.for	0605 Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	40	en_US

Abstract:

Abstract Samples of rhizosphere soil and bulk soil were collected from under Araucaria bidwillii in a tropical montane rainforest at Mount Lewis forest reserve, Queensland, Australia. DNA was extracted from soil and denaturing gradient gel electrophoresis (DGGE) profiles of PCRamplified partial rDNA internal transcribed spacer (ITS) regions of rhizosphere and bulk soil samples compared. Neither canonical analysis of the principal coordinates nor principal component analysis revealed clear separation of soil samples according to their origin in rhizosphere or bulk soil. Following amplification of rDNA ITS regions using the primers ITS1-F and ITS4, restriction fragment length polymorphism (RFLP) and sequence analyses were also used to identify fungal sequences that were present in clone assemblages from rhizosphere soil and bulk soil. A total of fifty-three RFLP types were thus identified, with the majority being ascomycetes and the remainder basidiomycetes or zygomycetes. Nine RFLP types had affinity with Mortierellaceae, and phylogenetic analysis indicated that these probably represent multiple Mortierella species in both rhizosphere and bulk soil samples. Similar evidence was obtained for the presence of multiple Trichosporon species. RFLP type 53 from a bulk soil sample was identified as a Soil Clone Group I ascomycete, confirming the widespread occurrence of this group. © Kevin D. Hyde 2009.

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http://hdl.handle.net/10453/28221