Epidermal cell differentiation in cotton mediated by the homeodomain leucine zipper gene, GhHD-1

Walford, SA; Wu, Y; Llewellyn, DJ; Dennis, ES

Epidermal cell differentiation in cotton mediated by the homeodomain leucine zipper gene, GhHD-1

Walford, SA Wu, Y Llewellyn, DJ Dennis, ES

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Publication Type:: Journal Article
Citation:: Plant Journal, 2012, 71 (3), pp. 464 - 478
Issue Date:: 2012-08-01

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Field	Value	Language
dc.contributor.author	Walford, SA	en_US
dc.contributor.author	Wu, Y	en_US
dc.contributor.author	Llewellyn, DJ	en_US
dc.contributor.author	Dennis, ES https://orcid.org/0000-0002-7850-591X	en_US
dc.date.issued	2012-08-01	en_US
dc.identifier.citation	Plant Journal, 2012, 71 (3), pp. 464 - 478	en_US
dc.identifier.issn	0960-7412	en_US
dc.identifier.uri	http://hdl.handle.net/10453/28702
dc.description.abstract	Gossypium hirsutum L. (cotton) fibres are specialized trichomes a few centimetres in length that grow from the seed coat. Few genes directly involved in the differentiation of these epidermal cells have been identified. These include GhMYB25-like and GhMYB25, two related MYB transcription factors that regulate fibre cell initiation and expansion. We have also identified a putative homeodomain leucine zipper (HD-ZIP) transcription factor, GhHD-1, expressed in trichomes and early fibres that might play a role in cotton fibre initiation. Here, we characterize GhHD-1 homoeologues from tetraploid G. hirsutum and show, using reporter constructs and quantitative real-time PCR (qRT-PCR), that they are expressed predominantly in epidermal tissues during early fibre development, and in other tissues bearing epidermal trichomes. Silencing of GhHD-1 reduced trichome formation and delayed the timing of fibre initiation. Constitutive overexpression of GhHD-1 increased the number of fibres initiating on the seed, but did not affect leaf trichomes. Expression of GhHD-1 in cotton silenced for different fibre MYBs suggest that in ovules it acts downstream of GhMYB25-like, but is unaffected in GhMYB25- or GhMYB109-silenced plants. Microarray analysis of silencing and overexpression lines of GhHD-1 indicated that it potentially regulates the levels of ethylene and reactive oxidation species (ROS) through a WRKY transcription factor and calcium-signalling pathway genes to activate downstream genes necessary for cell expansion and elongation. © 2012 CSIRO.	en_US
dc.relation.ispartof	Plant Journal	en_US
dc.relation.isbasedon	10.1111/j.1365-313X.2012.05003.x	en_US
dc.subject.classification	Plant Biology & Botany	en_US
dc.subject.mesh	Plants, Genetically Modified	en_US
dc.subject.mesh	Gossypium	en_US
dc.subject.mesh	Plant Components, Aerial	en_US
dc.subject.mesh	Seeds	en_US
dc.subject.mesh	Plant Epidermis	en_US
dc.subject.mesh	Reactive Oxygen Species	en_US
dc.subject.mesh	Ethylenes	en_US
dc.subject.mesh	Plant Proteins	en_US
dc.subject.mesh	Transcription Factors	en_US
dc.subject.mesh	Sequence Alignment	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	Cell Differentiation	en_US
dc.subject.mesh	Cell Enlargement	en_US
dc.subject.mesh	Calcium Signaling	en_US
dc.subject.mesh	Gene Expression Regulation, Developmental	en_US
dc.subject.mesh	Gene Expression Regulation, Plant	en_US
dc.subject.mesh	Amino Acid Sequence	en_US
dc.subject.mesh	Leucine Zippers	en_US
dc.subject.mesh	Genes, Homeobox	en_US
dc.subject.mesh	Time Factors	en_US
dc.subject.mesh	Cotton Fiber	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.subject.mesh	Promoter Regions, Genetic	en_US
dc.title	Epidermal cell differentiation in cotton mediated by the homeodomain leucine zipper gene, GhHD-1	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	71	en_US
utslib.for	0607 Plant Biology	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	71	en_US

Abstract:

Gossypium hirsutum L. (cotton) fibres are specialized trichomes a few centimetres in length that grow from the seed coat. Few genes directly involved in the differentiation of these epidermal cells have been identified. These include GhMYB25-like and GhMYB25, two related MYB transcription factors that regulate fibre cell initiation and expansion. We have also identified a putative homeodomain leucine zipper (HD-ZIP) transcription factor, GhHD-1, expressed in trichomes and early fibres that might play a role in cotton fibre initiation. Here, we characterize GhHD-1 homoeologues from tetraploid G. hirsutum and show, using reporter constructs and quantitative real-time PCR (qRT-PCR), that they are expressed predominantly in epidermal tissues during early fibre development, and in other tissues bearing epidermal trichomes. Silencing of GhHD-1 reduced trichome formation and delayed the timing of fibre initiation. Constitutive overexpression of GhHD-1 increased the number of fibres initiating on the seed, but did not affect leaf trichomes. Expression of GhHD-1 in cotton silenced for different fibre MYBs suggest that in ovules it acts downstream of GhMYB25-like, but is unaffected in GhMYB25- or GhMYB109-silenced plants. Microarray analysis of silencing and overexpression lines of GhHD-1 indicated that it potentially regulates the levels of ethylene and reactive oxidation species (ROS) through a WRKY transcription factor and calcium-signalling pathway genes to activate downstream genes necessary for cell expansion and elongation. © 2012 CSIRO.

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http://hdl.handle.net/10453/28702