Correlations between oxygen affinity and sequence classifications of plant hemoglobins

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Journal Article
Biopolymers - Peptide Science Section, 2009, 91 (12), pp. 1083 - 1096
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Plants express three phylogenetic classes of hemoglobins (Hb) based on sequence analyses. Class 1 and 2 Hbs are full-length globins with the classical eight helix Mb-like fold, whereas Class 3 plant Hbs resemble the truncated globins found in bacteria. With the exception of the specialized leghemoglobins, the physiological functions of these plant hemoglobins remain unknown. We have reviewed and, in some cases, measured new oxygen binding properties of a large number of Class 1 and 2 plant nonsymbiotic Hbs and leghemoglobins. We found that sequence classification correlates with distinct extents of hexacoordination with the distal histidine and markedly different overall oxygen affinities and association and dissociation rate constants. These results suggest strong selective pressure for the evolution of distinct physiological functions. The leghemoglobins evolved from the Class 2 globins and show no hexacoordination, very high rates of O2binding (∼250 μM-1s-1), moderately high rates of O2dissociation (∼5-15 s-1), and high oxygen affinity (Kdor P50≈ 50 nM). These properties both facilitate O2diffusion to respiring N2fixing bacteria and reduce O2tension in the root nodules of legumes. The Class 1 plant Hbs show weak hexacoordination (KHisE7≈ 2), moderate rates of O2binding (∼25 μM-1s-1), very small rates of O2dissociation (∼0.16 s-1), and remarkably high O2affinities (P50≈ 2 nM), suggesting a function involving O2and nitric oxide (NO) scavenging. The Class 2 Hbs exhibit strong hexacoordination (KHisE7≈ 100), low rates of O2binding (∼1 μM-1s-1), moderately low O2dissociation rate constants (∼1 s-1), and moderate, Mb-like O2affinities (P50≈ 340 nM), perhaps suggesting a sensing role for sustained low, micromolar levels of oxygen. © 2009 Wiley Periodicals, Inc.
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