Vernalization insensitive 3 (VIN3) is required for the response of Arabidopsis thaliana seedlings exposed to low oxygen conditions

Bond, DM; Wilson, IW; Dennis, ES; Pogson, BJ; Jean Finnegan, E

Vernalization insensitive 3 (VIN3) is required for the response of Arabidopsis thaliana seedlings exposed to low oxygen conditions

Bond, DM Wilson, IW Dennis, ES

Pogson, BJ Jean Finnegan, E

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Publication Type:: Journal Article
Citation:: Plant Journal, 2009, 59 (4), pp. 576 - 587
Issue Date:: 2009-08-01

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Field	Value	Language
dc.contributor.author	Bond, DM	en_US
dc.contributor.author	Wilson, IW	en_US
dc.contributor.author	Dennis, ES https://orcid.org/0000-0002-7850-591X	en_US
dc.contributor.author	Pogson, BJ	en_US
dc.contributor.author	Jean Finnegan, E	en_US
dc.date.issued	2009-08-01	en_US
dc.identifier.citation	Plant Journal, 2009, 59 (4), pp. 576 - 587	en_US
dc.identifier.issn	0960-7412	en_US
dc.identifier.uri	http://hdl.handle.net/10453/28707
dc.description.abstract	VERNALIZATION INSENSITIVE 3 (VIN3), which is required for the vernalization-mediated epigenetic repression of FLOWERING LOCUS C (FLC) in Arabidopsis thaliana, is quantitatively induced in response to low temperatures. We found that hypoxic conditions also induce VIN3 in a quantitative manner but high salt, high temperatures and osmotic stress do not. Inhibition of mitochondrial respiration did not induce VIN3 expression, consistent with the lack of VIN3 induction in response to other stresses that affect the rate of mitochondrial respiration. De novo protein synthesis is required for VIN3 induction during hypoxic conditions; this situation is not the case for VIN3 induction by low temperatures, indicating that different mechanisms act to induce VIN3 expression in response to cold and hypoxic conditions. Without VIN3 activity, fewer seedlings survived following a 72-h period of hypoxic treatment, indicating that VIN3 is required for the survival of Arabidopsis thaliana in response to hypoxic stress. Complementation of the vin3 mutant with a VIN3 transgene restored the wild-type response to low oxygen and confirmed the role of VIN3 in protecting both shoots and roots during low oxygen conditions. Loss of VIN3 protein did not affect the transcriptional regulation of genes known to be important in the response to low oxygen stress, which suggests that there is a novel mechanism to combat hypoxia that involves VIN3. This mechanism is likely to involve chromatin remodelling and may be similar to the role of VIN3 in the epigenetic repression of FLC during the vernalization response. © 2009 Blackwell Publishing Ltd.	en_US
dc.relation.ispartof	Plant Journal	en_US
dc.relation.isbasedon	10.1111/j.1365-313X.2009.03891.x	en_US
dc.subject.classification	Plant Biology & Botany	en_US
dc.subject.mesh	Mitochondria	en_US
dc.subject.mesh	Plants, Genetically Modified	en_US
dc.subject.mesh	Arabidopsis	en_US
dc.subject.mesh	Seedling	en_US
dc.subject.mesh	Sodium Chloride	en_US
dc.subject.mesh	Oxygen	en_US
dc.subject.mesh	Cycloheximide	en_US
dc.subject.mesh	DNA-Binding Proteins	en_US
dc.subject.mesh	Arabidopsis Proteins	en_US
dc.subject.mesh	Transcription Factors	en_US
dc.subject.mesh	RNA, Plant	en_US
dc.subject.mesh	Oligonucleotide Array Sequence Analysis	en_US
dc.subject.mesh	Genetic Complementation Test	en_US
dc.subject.mesh	Cell Hypoxia	en_US
dc.subject.mesh	Gene Expression Regulation, Plant	en_US
dc.subject.mesh	Osmotic Pressure	en_US
dc.subject.mesh	Cold Temperature	en_US
dc.subject.mesh	Hot Temperature	en_US
dc.subject.mesh	Seedlings	en_US
dc.title	Vernalization insensitive 3 (VIN3) is required for the response of Arabidopsis thaliana seedlings exposed to low oxygen conditions	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.citation.volume	59	en_US
utslib.for	0607 Plant Biology	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	4	en_US
pubs.publication-status	Published	en_US
pubs.volume	59	en_US

Abstract:

VERNALIZATION INSENSITIVE 3 (VIN3), which is required for the vernalization-mediated epigenetic repression of FLOWERING LOCUS C (FLC) in Arabidopsis thaliana, is quantitatively induced in response to low temperatures. We found that hypoxic conditions also induce VIN3 in a quantitative manner but high salt, high temperatures and osmotic stress do not. Inhibition of mitochondrial respiration did not induce VIN3 expression, consistent with the lack of VIN3 induction in response to other stresses that affect the rate of mitochondrial respiration. De novo protein synthesis is required for VIN3 induction during hypoxic conditions; this situation is not the case for VIN3 induction by low temperatures, indicating that different mechanisms act to induce VIN3 expression in response to cold and hypoxic conditions. Without VIN3 activity, fewer seedlings survived following a 72-h period of hypoxic treatment, indicating that VIN3 is required for the survival of Arabidopsis thaliana in response to hypoxic stress. Complementation of the vin3 mutant with a VIN3 transgene restored the wild-type response to low oxygen and confirmed the role of VIN3 in protecting both shoots and roots during low oxygen conditions. Loss of VIN3 protein did not affect the transcriptional regulation of genes known to be important in the response to low oxygen stress, which suggests that there is a novel mechanism to combat hypoxia that involves VIN3. This mechanism is likely to involve chromatin remodelling and may be similar to the role of VIN3 in the epigenetic repression of FLC during the vernalization response. © 2009 Blackwell Publishing Ltd.

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http://hdl.handle.net/10453/28707