ATP Synthase Repression In Tobacco Restricts Photosynthetic Electron Transport, CO2 Assimilation, And Plant Growth By Overacidification Of The Thylakoid Lumen

Rott, M; Martins, N; Thiele, W; Lein, W; Bock, R; Kramer, D; Schottler, M

ATP Synthase Repression In Tobacco Restricts Photosynthetic Electron Transport, CO2 Assimilation, And Plant Growth By Overacidification Of The Thylakoid Lumen

Rott, M Martins, N Thiele, W Lein, W Bock, R Kramer, D Schottler, M

Permalink

Publisher:: Amer Soc Plant Biologists
Publication Type:: Journal Article
Citation:: The Plant Cell, 2011, 23 (1), pp. 304 - 321
Issue Date:: 2011-01

Closed Access

	Filename	Description	Size
	2012002541OK.pdf		1.17 MB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Rott, M	en_US
dc.contributor.author	Martins, N	en_US
dc.contributor.author	Thiele, W	en_US
dc.contributor.author	Lein, W	en_US
dc.contributor.author	Bock, R	en_US
dc.contributor.author	Kramer, D	en_US
dc.contributor.author	Schottler, M	en_US
dc.date.issued	2011-01	en_US
dc.identifier.citation	The Plant Cell, 2011, 23 (1), pp. 304 - 321	en_US
dc.identifier.issn	1040-4651	en_US
dc.identifier.uri	http://hdl.handle.net/10453/28873
dc.description.abstract	Tobacco (Nicotiana tabacum) plants strictly adjust the contents of both ATP synthase and cytochrome b(6)f complex to the metabolic demand for ATP and NADPH. While the cytochrome b(6)f complex catalyzes the rate-limiting step of photosynthetic electron flux and thereby controls assimilation, the functional significance of the ATP synthase adjustment is unknown. Here, we reduced ATP synthase accumulation by an antisense approach directed against the essential nuclear-encoded gamma-subunit (AtpC) and by the introduction of point mutations into the translation initiation codon of the plastid-encoded atpB gene (encoding the essential beta-subunit) via chloroplast transformation. Both strategies yielded transformants with ATP synthase contents ranging from 100 to <10% of wild-type levels. While the accumulation of the components of the linear electron transport chain was largely unaltered, linear electron flux was strongly inhibited due to decreased rates of plastoquinol reoxidation at the cytochrome b(6)f complex (photosynthetic control). Also, nonphotochemical quenching was triggered at very low light intensities, strongly reducing the quantum efficiency of CO2 fixation. We show evidence that this is due to an increased steady state proton motive force, resulting in strong lumen overacidification, which in turn represses photosynthesis due to photosynthetic control and dissipation of excitation energy in the antenna bed.	en_US
dc.publisher	Amer Soc Plant Biologists	en_US
dc.relation.ispartof	The Plant Cell	en_US
dc.relation.isbasedon	10.1105/tpc.110.079111	en_US
dc.subject.classification	Plant Biology & Botany	en_US
dc.title	ATP Synthase Repression In Tobacco Restricts Photosynthetic Electron Transport, CO2 Assimilation, And Plant Growth By Overacidification Of The Thylakoid Lumen	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	23	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	0607 Plant Biology	en_US
utslib.for	0604 Genetics	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access
pubs.consider-herdc	false	en_US
pubs.issue	1	en_US
pubs.volume	23	en_US

Abstract:

Tobacco (Nicotiana tabacum) plants strictly adjust the contents of both ATP synthase and cytochrome b(6)f complex to the metabolic demand for ATP and NADPH. While the cytochrome b(6)f complex catalyzes the rate-limiting step of photosynthetic electron flux and thereby controls assimilation, the functional significance of the ATP synthase adjustment is unknown. Here, we reduced ATP synthase accumulation by an antisense approach directed against the essential nuclear-encoded gamma-subunit (AtpC) and by the introduction of point mutations into the translation initiation codon of the plastid-encoded atpB gene (encoding the essential beta-subunit) via chloroplast transformation. Both strategies yielded transformants with ATP synthase contents ranging from 100 to <10% of wild-type levels. While the accumulation of the components of the linear electron transport chain was largely unaltered, linear electron flux was strongly inhibited due to decreased rates of plastoquinol reoxidation at the cytochrome b(6)f complex (photosynthetic control). Also, nonphotochemical quenching was triggered at very low light intensities, strongly reducing the quantum efficiency of CO2 fixation. We show evidence that this is due to an increased steady state proton motive force, resulting in strong lumen overacidification, which in turn represses photosynthesis due to photosynthetic control and dissipation of excitation energy in the antenna bed.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/28873