Shape-dependent cellular processing of polyelectrolyte capsules

Shimoni, O; Yan, Y; Wang, Y; Caruso, F

Shape-dependent cellular processing of polyelectrolyte capsules

Shimoni, O

Yan, Y Wang, Y Caruso, F

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Publication Type:: Journal Article
Citation:: ACS Nano, 2013, 7 (1), pp. 522 - 530
Issue Date:: 2013-01-22

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Field	Value	Language
dc.contributor.author	Shimoni, O https://orcid.org/0000-0001-8822-1024	en_US
dc.contributor.author	Yan, Y	en_US
dc.contributor.author	Wang, Y	en_US
dc.contributor.author	Caruso, F	en_US
dc.date.issued	2013-01-22	en_US
dc.identifier.citation	ACS Nano, 2013, 7 (1), pp. 522 - 530	en_US
dc.identifier.issn	1936-0851	en_US
dc.identifier.uri	http://hdl.handle.net/10453/28985
dc.description.abstract	Particle shape is emerging as a key design parameter for tailoring the interactions between particles and cells. Herein, we report the preparation of rod-shaped layer-by-layer (LbL)-assembled polymer hydrogel capsules with tunable aspect ratios (ARs). By templating spherical and rodlike silica particles, disulfide-stabilized poly(methacrylic acid) hydrogel capsules (PMA HCs) with different ARs (from 1 to 4) are generated. The influence of capsule AR on cellular internalization and intracellular fate was quantitatively investigated by flow cytometry, imaging flow cytometry, and fluorescence deconvolution microscopy. These experiments reveal that the cellular internalization kinetics of PMA HCs are dependent on the AR, with spherical capsules being internalized more rapidly and to a greater extent compared with rod-shaped capsules. In contrast, the capsules with different ARs are colocalized with the lysosomal marker LAMP1, suggesting that the lysosomal compartmentalization is independent of shape for these soft polymer capsules. © 2012 American Chemical Society.	en_US
dc.relation.ispartof	ACS Nano	en_US
dc.relation.isbasedon	10.1021/nn3046117	en_US
dc.subject.classification	Nanoscience & Nanotechnology	en_US
dc.subject.mesh	Hela Cells	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Electrolytes	en_US
dc.subject.mesh	Microscopy, Fluorescence	en_US
dc.subject.mesh	Materials Testing	en_US
dc.subject.mesh	Nanocapsules	en_US
dc.subject.mesh	Cell Tracking	en_US
dc.subject.mesh	HeLa Cells	en_US
dc.title	Shape-dependent cellular processing of polyelectrolyte capsules	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	7	en_US
utslib.for	090301 Biomaterials	en_US
utslib.for	030603 Colloid and Surface Chemistry	en_US
utslib.for	030301 Chemical Characterisation of Materials	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	7	en_US

Abstract:

Particle shape is emerging as a key design parameter for tailoring the interactions between particles and cells. Herein, we report the preparation of rod-shaped layer-by-layer (LbL)-assembled polymer hydrogel capsules with tunable aspect ratios (ARs). By templating spherical and rodlike silica particles, disulfide-stabilized poly(methacrylic acid) hydrogel capsules (PMA HCs) with different ARs (from 1 to 4) are generated. The influence of capsule AR on cellular internalization and intracellular fate was quantitatively investigated by flow cytometry, imaging flow cytometry, and fluorescence deconvolution microscopy. These experiments reveal that the cellular internalization kinetics of PMA HCs are dependent on the AR, with spherical capsules being internalized more rapidly and to a greater extent compared with rod-shaped capsules. In contrast, the capsules with different ARs are colocalized with the lysosomal marker LAMP1, suggesting that the lysosomal compartmentalization is independent of shape for these soft polymer capsules. © 2012 American Chemical Society.

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http://hdl.handle.net/10453/28985