Chlorophyll-normalized isoprene production in laboratory cultures of marine microalgae and implications for global models

Exton, DA; Suggett, DJ; McGenity, TJ; Steinke, M

Chlorophyll-normalized isoprene production in laboratory cultures of marine microalgae and implications for global models

Exton, DA Suggett, DJ

McGenity, TJ Steinke, M

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Publication Type:: Journal Article
Citation:: Limnology and Oceanography, 2013, 58 (4), pp. 1301 - 1311
Issue Date:: 2013-06-13

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Field	Value	Language
dc.contributor.author	Exton, DA	en_US
dc.contributor.author	Suggett, DJ https://orcid.org/0000-0001-5326-2520	en_US
dc.contributor.author	McGenity, TJ	en_US
dc.contributor.author	Steinke, M	en_US
dc.date.issued	2013-06-13	en_US
dc.identifier.citation	Limnology and Oceanography, 2013, 58 (4), pp. 1301 - 1311	en_US
dc.identifier.issn	0024-3590	en_US
dc.identifier.uri	http://hdl.handle.net/10453/29123
dc.description.abstract	We used laboratory cultures of marine microalgae to investigate the effects of growth conditions and their taxonomic position on the production of isoprene, a gas that has major effects on atmospheric chemistry and provides stress tolerance to many primary producers. Isoprene was quantified from 21 microalgal strains sampled during exponential growth, using purge-and-trap pre-concentration and gas chromatography with flameionization detection. Isoprene production rates varied by two orders of magnitude between strains (0.03-1.34 μmol [g chlorophyll a]-1 h-1), and were positively correlated with temperature (r2 = 0.52, p < 0.001, n = 59). Three distinct sea surface temperature (SST)-dependent relationships were found between isoprene and chlorophyll a (μmol [g chlorophyll a]-1 h-1), an improvement in resolution over the single relationship used in previous models: for three polar strains grown at -1°C (slope = 0.03, R2 = 0.76, p < 0.05, n = 9), nine strains grown at 16°C (slope = 0.24, R2 = 0.43, p < 0.05, n = 27 with Dunaliella tertiolecta excluded), and eight strains grown at 26°C (slope = 0.39, R2 = 0.15, p < 0.05, n = 24). We then used a simple model that applied the SSTdependent nature of isoprene production to three representative bioregions for the growth temperatures used in this study. This approach yielded an estimate of global marine isoprene production that was 51% higher than previous attempts using an SST-independent single relationship. Taking into account the effect of temperature therefore potentially allows more precise modeling of marine isoprene production, and suggests that increasing the SST-based resolution of data beyond the three groups used here could further improve future modeling simulations. © 2013, by the Association for the Sciences of Limnology and Oceanography, Inc.	en_US
dc.relation.ispartof	Limnology and Oceanography	en_US
dc.relation.isbasedon	10.4319/lo.2013.58.4.1301	en_US
dc.subject.classification	Marine Biology & Hydrobiology	en_US
dc.title	Chlorophyll-normalized isoprene production in laboratory cultures of marine microalgae and implications for global models	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.citation.volume	58	en_US
utslib.for	0602 Ecology	en_US
utslib.for	0607 Plant Biology	en_US
utslib.for	04 Earth Sciences	en_US
utslib.for	05 Environmental Sciences	en_US
utslib.for	06 Biological Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	open_access
pubs.issue	4	en_US
pubs.publication-status	Published	en_US
pubs.volume	58	en_US

Abstract:

We used laboratory cultures of marine microalgae to investigate the effects of growth conditions and their taxonomic position on the production of isoprene, a gas that has major effects on atmospheric chemistry and provides stress tolerance to many primary producers. Isoprene was quantified from 21 microalgal strains sampled during exponential growth, using purge-and-trap pre-concentration and gas chromatography with flameionization detection. Isoprene production rates varied by two orders of magnitude between strains (0.03-1.34 μmol [g chlorophyll a]-1 h-1), and were positively correlated with temperature (r2 = 0.52, p < 0.001, n = 59). Three distinct sea surface temperature (SST)-dependent relationships were found between isoprene and chlorophyll a (μmol [g chlorophyll a]-1 h-1), an improvement in resolution over the single relationship used in previous models: for three polar strains grown at -1°C (slope = 0.03, R2 = 0.76, p < 0.05, n = 9), nine strains grown at 16°C (slope = 0.24, R2 = 0.43, p < 0.05, n = 27 with Dunaliella tertiolecta excluded), and eight strains grown at 26°C (slope = 0.39, R2 = 0.15, p < 0.05, n = 24). We then used a simple model that applied the SSTdependent nature of isoprene production to three representative bioregions for the growth temperatures used in this study. This approach yielded an estimate of global marine isoprene production that was 51% higher than previous attempts using an SST-independent single relationship. Taking into account the effect of temperature therefore potentially allows more precise modeling of marine isoprene production, and suggests that increasing the SST-based resolution of data beyond the three groups used here could further improve future modeling simulations. © 2013, by the Association for the Sciences of Limnology and Oceanography, Inc.

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http://hdl.handle.net/10453/29123