Heterosis of Arabidopsis hybrids between C24 and Col is associated with increased photosynthesis capacity

Fujimoto, R; Taylor, JM; Shirasawa, S; Peacock, WJ; Dennis, ES

Heterosis of Arabidopsis hybrids between C24 and Col is associated with increased photosynthesis capacity

Fujimoto, R Taylor, JM Shirasawa, S Peacock, WJ

Dennis, ES

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Publication Type:: Journal Article
Citation:: Proceedings of the National Academy of Sciences of the United States of America, 2012, 109 (18), pp. 7109 - 7114
Issue Date:: 2012-05-01

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Field	Value	Language
dc.contributor.author	Fujimoto, R	en_US
dc.contributor.author	Taylor, JM	en_US
dc.contributor.author	Shirasawa, S	en_US
dc.contributor.author	Peacock, WJ https://orcid.org/0000-0001-7314-9074	en_US
dc.contributor.author	Dennis, ES https://orcid.org/0000-0002-7850-591X	en_US
dc.date.issued	2012-05-01	en_US
dc.identifier.citation	Proceedings of the National Academy of Sciences of the United States of America, 2012, 109 (18), pp. 7109 - 7114	en_US
dc.identifier.issn	0027-8424	en_US
dc.identifier.uri	http://hdl.handle.net/10453/29288
dc.description.abstract	Arabidopsis thaliana shows hybrid vigor (heterosis) in progeny of crosses between Columbia-0 and C24 accessions. Hybrid vigor was evident as early as mature seeds and in seedlings 3 d after sowing (DAS). At 3 DAS, genes encoding chloroplast-located proteins were significantly overrepresented (187) among the 724 genes that have greater than midparent values of expression in the hybrid. Many of these genes are involved in chlorophyll biosynthesis and photosynthesis. The rate of photosynthesis was constant per unit leaf area in parents and hybrids. Larger cell sizes in the hybrids were associated with more chloroplasts per cell, more total chlorophyll, and more photosynthesis. The increased transcription of the chloroplast-targeted genes was restricted to the 3-7 DAS period. At 10 DAS, only 118 genes had expression levels different from the expected midparent value in the hybrid, and only 12 of these genes were differentially expressed at 3 DAS. The early increase in activity of genes involved in photosynthesis and the associated phenomena of increases in cell size and number through development, leading to larger leaf areas of all leaves in the hybrid, suggest a central role for increased photosynthesis in the production of the heterotic biomass. In support of this correlation, we found that an inhibitor of photosynthesis eliminated heterosis and that higher light intensities enhanced both photosynthesis and heterosis. In hybrids with low-level heterosis (Landsberg erecta x Columbia-0), chloroplast-targeted genes were not up-regulated and leaf areas were only marginally increased.	en_US
dc.relation.ispartof	Proceedings of the National Academy of Sciences of the United States of America	en_US
dc.relation.isbasedon	10.1073/pnas.1204464109	en_US
dc.subject.mesh	Arabidopsis	en_US
dc.subject.mesh	Plant Leaves	en_US
dc.subject.mesh	Chlorophyll	en_US
dc.subject.mesh	Cell Count	en_US
dc.subject.mesh	Biomass	en_US
dc.subject.mesh	Cell Size	en_US
dc.subject.mesh	Photosynthesis	en_US
dc.subject.mesh	Gene Expression Regulation, Plant	en_US
dc.subject.mesh	Hybrid Vigor	en_US
dc.subject.mesh	Genes, Plant	en_US
dc.title	Heterosis of Arabidopsis hybrids between C24 and Col is associated with increased photosynthesis capacity	en_US
dc.type	Journal Article
utslib.citation.volume	18	en_US
utslib.citation.volume	109	en_US
utslib.for	0607 Plant Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	18	en_US
pubs.publication-status	Published	en_US
pubs.volume	109	en_US

Abstract:

Arabidopsis thaliana shows hybrid vigor (heterosis) in progeny of crosses between Columbia-0 and C24 accessions. Hybrid vigor was evident as early as mature seeds and in seedlings 3 d after sowing (DAS). At 3 DAS, genes encoding chloroplast-located proteins were significantly overrepresented (187) among the 724 genes that have greater than midparent values of expression in the hybrid. Many of these genes are involved in chlorophyll biosynthesis and photosynthesis. The rate of photosynthesis was constant per unit leaf area in parents and hybrids. Larger cell sizes in the hybrids were associated with more chloroplasts per cell, more total chlorophyll, and more photosynthesis. The increased transcription of the chloroplast-targeted genes was restricted to the 3-7 DAS period. At 10 DAS, only 118 genes had expression levels different from the expected midparent value in the hybrid, and only 12 of these genes were differentially expressed at 3 DAS. The early increase in activity of genes involved in photosynthesis and the associated phenomena of increases in cell size and number through development, leading to larger leaf areas of all leaves in the hybrid, suggest a central role for increased photosynthesis in the production of the heterotic biomass. In support of this correlation, we found that an inhibitor of photosynthesis eliminated heterosis and that higher light intensities enhanced both photosynthesis and heterosis. In hybrids with low-level heterosis (Landsberg erecta x Columbia-0), chloroplast-targeted genes were not up-regulated and leaf areas were only marginally increased.

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http://hdl.handle.net/10453/29288