Composition, uniqueness and variability of the epiphytic bacterial community of the green alga Ulva australis

Burke, C; Thomas, T; Lewis, M; Steinberg, P; Kjelleberg, S

Composition, uniqueness and variability of the epiphytic bacterial community of the green alga Ulva australis

Burke, C

Thomas, T Lewis, M Steinberg, P Kjelleberg, S

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Publication Type:: Journal Article
Citation:: ISME Journal, 2011, 5 (4), pp. 590 - 600
Issue Date:: 2011-04-01

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Field	Value	Language
dc.contributor.author	Burke, C https://orcid.org/0000-0002-8917-0019	en_US
dc.contributor.author	Thomas, T	en_US
dc.contributor.author	Lewis, M	en_US
dc.contributor.author	Steinberg, P	en_US
dc.contributor.author	Kjelleberg, S	en_US
dc.date.issued	2011-04-01	en_US
dc.identifier.citation	ISME Journal, 2011, 5 (4), pp. 590 - 600	en_US
dc.identifier.issn	1751-7362	en_US
dc.identifier.uri	http://hdl.handle.net/10453/29691
dc.description.abstract	Green Ulvacean marine macroalgae are distributed worldwide in coastal tidal and subtidal ecosystems. As for many living surfaces in the marine environment, little is known concerning the epiphytic bacterial biofilm communities that inhabit algal surfaces. This study reports on the largest published libraries of near full-length 16S rRNA genes from a marine algal surface (5293 sequences from six samples) allowing for an in-depth assessment of the diversity and phylogenetic profile of the bacterial community on a green Ulvacean alga. Large 16S rRNA gene libraries of surrounding seawater were also used to determine the uniqueness of this bacterial community. The surface of Ulva australis is dominated by sequences of Alphaproteobacteria and the Bacteroidetes, especially within the Rhodobacteriaceae, Sphingomonadaceae, Flavobacteriaceae and Sapropiraceae families. Seawater libraries were also dominated by Alphaproteobacteria and Bacteroidetes sequences, but were shown to be clearly distinct from U. australis libraries through the clustering of sequences into operational taxonomic units and Bray-Curtis similarity analysis. Almost no similarity was observed between these two environments at the species level, and only minor similarity was observed at levels of sequence clustering representing clades of bacteria within family and genus taxonomic groups. Variability between libraries of U. australis was relatively high, and a consistent sub-population of bacterial species was not detected. The competitive lottery model, originally derived to explain diversity in coral reef fishes, may explain the pattern of colonization of this algal surface. © 2011 International Society for Microbial Ecology All rights reserved.	en_US
dc.relation.ispartof	ISME Journal	en_US
dc.relation.isbasedon	10.1038/ismej.2010.164	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Ulva	en_US
dc.subject.mesh	Bacteria	en_US
dc.subject.mesh	Bacteroidetes	en_US
dc.subject.mesh	Alphaproteobacteria	en_US
dc.subject.mesh	RNA, Ribosomal, 16S	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Ecosystem	en_US
dc.subject.mesh	Seawater	en_US
dc.subject.mesh	Phylogeny	en_US
dc.title	Composition, uniqueness and variability of the epiphytic bacterial community of the green alga Ulva australis	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.citation.volume	5	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	05 Environmental Sciences	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	10 Technology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	4	en_US
pubs.publication-status	Published	en_US
pubs.volume	5	en_US

Abstract:

Green Ulvacean marine macroalgae are distributed worldwide in coastal tidal and subtidal ecosystems. As for many living surfaces in the marine environment, little is known concerning the epiphytic bacterial biofilm communities that inhabit algal surfaces. This study reports on the largest published libraries of near full-length 16S rRNA genes from a marine algal surface (5293 sequences from six samples) allowing for an in-depth assessment of the diversity and phylogenetic profile of the bacterial community on a green Ulvacean alga. Large 16S rRNA gene libraries of surrounding seawater were also used to determine the uniqueness of this bacterial community. The surface of Ulva australis is dominated by sequences of Alphaproteobacteria and the Bacteroidetes, especially within the Rhodobacteriaceae, Sphingomonadaceae, Flavobacteriaceae and Sapropiraceae families. Seawater libraries were also dominated by Alphaproteobacteria and Bacteroidetes sequences, but were shown to be clearly distinct from U. australis libraries through the clustering of sequences into operational taxonomic units and Bray-Curtis similarity analysis. Almost no similarity was observed between these two environments at the species level, and only minor similarity was observed at levels of sequence clustering representing clades of bacteria within family and genus taxonomic groups. Variability between libraries of U. australis was relatively high, and a consistent sub-population of bacterial species was not detected. The competitive lottery model, originally derived to explain diversity in coral reef fishes, may explain the pattern of colonization of this algal surface. © 2011 International Society for Microbial Ecology All rights reserved.

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http://hdl.handle.net/10453/29691