Identification of a nonselective cation channel in isolated lens fiber cells that is activated by cell shrinkage
- Publisher:
- American Physiological Society
- Publication Type:
- Journal Article
- Citation:
- American Journal of Physiology - Cell Physiology, 2012, 303 (12), pp. 1252 - 1259
- Issue Date:
- 2012-01
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| Filename | Description | Size | |||
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 | 2013005337OK.pdf | 3.63 MB |
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The initiation of lens cataract has long been associated with the development of a membrane leak in lens fiber cells that depolarizes the lens intracellular potential and elevates intracellular Na+ and Ca2+ concentrations. It has been proposed that the leak observed in cataractous lenses is due to the activation of a nonselective cation (NSC) conductance in the normal electrically tight fiber cells. Studies of the membrane properties of isolated fiber cells using the patch-clamp technique have demonstrated a differentiation-dependent shift in membrane permeability from K+-dominated in epithelial and short fiber cells toward larger contributions from anion and NSC conductances as fiber cells elongate. In this study, the NSC conductances in elongating lens fiber cells are demonstrated to be due to at least two distinct classes: a Gd3+-sensitive, mechanosensitive channel whose blockade is essential for obtaining viable isolated fiber cells, and a second Gd3+-insensitive, La3+-sensitive conductance that appears to be activated by cell shrinkage. This second conductance was eliminated by the replacement of extracellular Na+ with the impermeant cation N-methyl-D-glucamine and was potentiated by both hypertonic stress and isosmotic cell shrinkage evoked by the replacement of extracellular Cl- with the impermeant anion gluconate.
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