Contrasting roles of condensin I and condensin II in mitotic chromosome formation

Green, LC; Kalitsis, P; Chang, TM; Cipetic, M; Kim, JH; Marshall, O; Turnbull, L; Whitchurch, CB; Vagnarelli, P; Samejima, K; Earnshaw, WC; Choo, KHA; Hudson, DF

Contrasting roles of condensin I and condensin II in mitotic chromosome formation

Green, LC Kalitsis, P Chang, TM Cipetic, M Kim, JH Marshall, O Turnbull, L

Whitchurch, CB

Vagnarelli, P Samejima, K Earnshaw, WC Choo, KHA Hudson, DF

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Publication Type:: Journal Article
Citation:: Journal of Cell Science, 2012, 125 (6), pp. 1591 - 1604
Issue Date:: 2012-03-15

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Field	Value	Language
dc.contributor.author	Green, LC	en_US
dc.contributor.author	Kalitsis, P	en_US
dc.contributor.author	Chang, TM	en_US
dc.contributor.author	Cipetic, M	en_US
dc.contributor.author	Kim, JH	en_US
dc.contributor.author	Marshall, O	en_US
dc.contributor.author	Turnbull, L https://orcid.org/0000-0002-9255-9033	en_US
dc.contributor.author	Whitchurch, CB https://orcid.org/0000-0003-2296-3791	en_US
dc.contributor.author	Vagnarelli, P	en_US
dc.contributor.author	Samejima, K	en_US
dc.contributor.author	Earnshaw, WC	en_US
dc.contributor.author	Choo, KHA	en_US
dc.contributor.author	Hudson, DF	en_US
dc.date.issued	2012-03-15	en_US
dc.identifier.citation	Journal of Cell Science, 2012, 125 (6), pp. 1591 - 1604	en_US
dc.identifier.issn	0021-9533	en_US
dc.identifier.uri	http://hdl.handle.net/10453/30697
dc.description.abstract	In vertebrates, two condensin complexes exist, condensin I and condensin II, which have differing but unresolved roles in organizing mitotic chromosomes. To dissect accurately the role of each complex in mitosis, we have made and studied the first vertebrate conditional knockouts of the genes encoding condensin I subunit CAP-H and condensin II subunit CAP-D3 in chicken DT40 cells. Live-cell imaging reveals highly distinct segregation defects. CAP-D3 (condensin II) knockout results in masses of chromatincontaining anaphase bridges. CAP-H (condensin I)-knockout anaphases have a more subtle defect, with chromatids showing fine chromatin fibres that are associated with failure of cytokinesis and cell death. Super-resolution microscopy reveals that condensin-Idepleted mitotic chromosomes are wider and shorter, with a diffuse chromosome scaffold, whereas condensin-II-depleted chromosomes retain a more defined scaffold, with chromosomes more stretched and seemingly lacking in axial rigidity. We conclude that condensin II is required primarily to provide rigidity by establishing an initial chromosome axis around which condensin I can arrange loops of chromatin. © 2012.	en_US
dc.relation	http://purl.org/au-research/grants/nhmrc/GNT0571905
dc.relation.ispartof	Journal of Cell Science	en_US
dc.relation.isbasedon	10.1242/jcs.097790	en_US
dc.subject.classification	Developmental Biology	en_US
dc.subject.mesh	Cell Line, Tumor	en_US
dc.subject.mesh	Chromosomes	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Chickens	en_US
dc.subject.mesh	Multiprotein Complexes	en_US
dc.subject.mesh	DNA-Binding Proteins	en_US
dc.subject.mesh	Mitosis	en_US
dc.subject.mesh	Adenosine Triphosphatases	en_US
dc.subject.mesh	Gene Knockout Techniques	en_US
dc.title	Contrasting roles of condensin I and condensin II in mitotic chromosome formation	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.citation.volume	125	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
dcterms.isVersionOf	Free to read version at https://doi.org/10.1242/jcs.097790
pubs.issue	6	en_US
pubs.publication-status	Published	en_US
pubs.volume	125	en_US

Abstract:

In vertebrates, two condensin complexes exist, condensin I and condensin II, which have differing but unresolved roles in organizing mitotic chromosomes. To dissect accurately the role of each complex in mitosis, we have made and studied the first vertebrate conditional knockouts of the genes encoding condensin I subunit CAP-H and condensin II subunit CAP-D3 in chicken DT40 cells. Live-cell imaging reveals highly distinct segregation defects. CAP-D3 (condensin II) knockout results in masses of chromatincontaining anaphase bridges. CAP-H (condensin I)-knockout anaphases have a more subtle defect, with chromatids showing fine chromatin fibres that are associated with failure of cytokinesis and cell death. Super-resolution microscopy reveals that condensin-Idepleted mitotic chromosomes are wider and shorter, with a diffuse chromosome scaffold, whereas condensin-II-depleted chromosomes retain a more defined scaffold, with chromosomes more stretched and seemingly lacking in axial rigidity. We conclude that condensin II is required primarily to provide rigidity by establishing an initial chromosome axis around which condensin I can arrange loops of chromatin. © 2012.

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http://hdl.handle.net/10453/30697