The assembly and use of tethered bilayer lipid membranes (tBLMs).

Publisher:
Humana Press (Springer Imprint)
Publication Type:
Journal Article
Citation:
Methods in molecular biology (Clifton, N.J.), 2015, 1232 pp. 45 - 53
Issue Date:
2015-01
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Because they are firmly held in place, tethered bilayer lipid membranes (tBLMs) are considerably more robust than supported lipid bilayers such as black lipid membranes (BLMs) (Cornell et al. Nature 387(6633): 580-583, 1997). Here we describe the procedures required to assemble and test tethered lipid bilayers that can incorporate various lipid species, peptides, and ion channel proteins.
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